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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
21/06/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, W. R. de; PACHECO, T. F.; DUARTE, K. E.; SAMPAIO, B. L.; OLIVEIRA, P. A. de; MARTINS, P. M.; SANTIAGO, T. R.; FORMIGHIERI, E. F.; VINECKY, F.; RIBEIRO, A. P.; DIAS, B. B. A.; KOBAYASHI, A. K.; MITCHELL, R. A. C.; RODRIGUES, D. de S.; MOLINARI, H. B. C. |
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Afiliação: |
Wagner Rodrigo de Souza, Bolsista da Embrapa Agroenergia; THALYTA FRAGA PACHECO, CNPAE; Karoline Estefani Duarte, Bolsista da Embrapa Agroenergia; Bruno Leite Sampaio, Bolsista da Embrapa Agroenergia; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; Polyana Kelly Martins, Bolsista da Embrapa Agroenergia; Thaís Ribeiro Santiago, Bolsista da Embrapa Agroenergia; EDUARDO FERNANDES FORMIGHIERI, CNPAE; Felipe Vinecky; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; Rowan Andrew Craig Mitchell; DASCIANA DE SOUSA RODRIGUES, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane, MenosBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol productio... Mostrar Tudo |
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Palavras-Chave: |
Cell-wall acylation; Lignocellulosic feedstock. |
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Thesaurus Nal: |
Biofuels; Biomass; Sugarcane. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198722/1/Silencing-of-a-BAHD-acyltransferase.pdf
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Marc: |
LEADER 02739naa a2200349 a 4500
001 2109978
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aSOUZA, W. R. de
245 $aSilencing of a BAHD acyltransferase in sugarcane increases biomass digestibility.$h[electronic resource]
260 $c2019
520 $aBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane,
650 $aBiofuels
650 $aBiomass
650 $aSugarcane
653 $aCell-wall acylation
653 $aLignocellulosic feedstock
700 1 $aPACHECO, T. F.
700 1 $aDUARTE, K. E.
700 1 $aSAMPAIO, B. L.
700 1 $aOLIVEIRA, P. A. de
700 1 $aMARTINS, P. M.
700 1 $aSANTIAGO, T. R.
700 1 $aFORMIGHIERI, E. F.
700 1 $aVINECKY, F.
700 1 $aRIBEIRO, A. P.
700 1 $aDIAS, B. B. A.
700 1 $aKOBAYASHI, A. K.
700 1 $aMITCHELL, R. A. C.
700 1 $aRODRIGUES, D. de S.
700 1 $aMOLINARI, H. B. C.
773 $tBiotechnology for Biofuels$gv. 12, n. 111, p. 1-14, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 57 | |
| 4. |  | DIAS, B. B. A.; CUNHA, W. G.; MORAIS, L. S.; ARAGÃO, F. J. L. Introdução e expressão do gene da oxalato descarboxilase (OxDC) de Flamulina velutipes em alface (Lactuca sativa L.). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 67.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis as a model plant for functional genomic studies in C4 Crops. In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864). p. 49-64| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | RIBEIRO, A. P.; MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; ANDRADE, A. C.; MOLINARI, H. B. C. Overexpression of the MATE gene in Setaria viridis. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 11.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis: a tool for functional gene analysis in sugarcane. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 19.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 13. | | SANTOS, M. de O.; ROMANO, E.; YOTOKO, K. S. C.; TINOCO, M. L. P.; DIAS, B. B. A.; ARAGÃO, F. J. L. Characterization of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis. Plant Science, Limerick, v. 168, n. 3, p. 723-729, Mar. 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | DIAS, B. B. A.; CUNHA, W. G.; MORAIS, L. S.; VIANNA, G. R.; RECH, E. L.; CAPDEVILLE, G. de; ARAGÃO, F. J. L. Expression of an oxalate decarboxylase gene from Flammulina sp. in transgenic lettuce (Lactuca sativa) plants and resistance to Sclerotinia sclerotiorum. Plant Pathology, v. 55, p. 187-193, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | MARTINS, N. F.; PATIL, S.; MOLINARI, H. B. C.; DIAS, B. B. A.; MARTINS, M. T. B.; QUIRINO, B. F.; EMMERSON, Z.; AMOS, B.; MAY, S. Expression profiling using Affymetrix GeneChip Microarrays in sugarcane during leaf senescence. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: SBG, 2011. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 16. | | DUARTE, K. E.; VIEIRA, N. G.; MARTINS, P. K.; RIBEIRO, A. P.; DIAS, B. B. A.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; MARRACCINI, P.; ANDRADE, A. C. Genetic transformation of setaria viridis with CcUNK8 for enhanced drought tolerance. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 10.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | PEREIRA, V. M.; DIAS, B. B. A.; SANTIAGO, T. R.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; FAVARO, L. C. de L. Identification and expression of sugarcane culm-specific expansin genes. In: BRAZILIAN BIOENERGY AND TECNOLOGY CONFERENCE - BBEST, 2017, Campos do Jordão, SP. [Resumos ...]. Campinas: SBE, 2017. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | DUARTE, K. E.; VIEIRA, N. G.; MARTINS, P. K.; RIBEIRO, A. P.; DIAS, B. B. A.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; MARRACCINI, P.; ANDRADE, A. C. Identificação e caracterização de genes órfãos ("no hits") de café (Coffea Canephora), envolvidos na resposta à seca. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador, BA. Anais...Brasília: Consórcio Pesquisa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | JUNGMANN, L.; ALBINO, M. M. C.; DIAS, B. B. A.; VIANNA, G. R.; RECH, E. L.; FARIA, J. C. de; ARAGÃO, F. J. L. Desenvolvimento de sistema para obtenção de plantas de feijão resistentes ao vírus do mosaico dourado do feijoeiro. In: REUNIÃO NACIONAL DE PESQUISA DE FEIJÃO, 6., 1999, Salvador : Resumos expandidos. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 1999. v.1. p. 42-43. (Embrapa Arroz e Feijão. Documentos, 99).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 20. | | JUNGMANN, L. C.; ALBINO, M. M. C.; DIAS, B. B. A.; VIANNA, G. R.; RECH, E. L.; FARIA, J. C.; ARAGÃO, F. J. L. Desenvolvimento de sistema para obtenção de plantas de feijão resistentes ao vírus do mosaico dourado do feijoeiro. In: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília. Anais: resumos dos trabalhos. Brasília, DF: EMBRAPA-CENARGEN, 1999. p. 48.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 57 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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