Data/hora: 09/06/2024 16:47:55

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 29/11/2018
Data da última atualização: | 25/06/2019
Autoria: | MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C.
Afiliação: | BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE.
Título: | Setaria viridis as a model plant for functional genomic studies in C4 Crops.
Ano de publicação: | 2019
Fonte/Imprenta: | In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864).
Páginas: | p. 49-64
Idioma: | Inglês
Conteúdo: | Setaria viridis is an emerging model for C4 species, and it is an important model to validate some genes for further C4 crop transformation, such as sugarcane, maize, and wheat. Here, we describe two protocols for stable transformation of S. viridis mediated by Agrobacterium tumefaciens with three different reporter genes and two selectable markers. Routine transformation efficiency reaching 29% was achieved using embryogenic callus in S. viridis (accession A10.1). Alternatively, we developed a transformation method by floral dip with 0.6% efficiency. The developed protocols could be useful for genetic and genomics studies of important food-feed-fiber-fuel C4 crops.
Palavras-Chave: | Embryogenic callus; Floral dip; Transformation.
Thesaurus Nal: | Agrobacterium; Grasses.
Categoria do assunto: | --
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (3427-1) - UPC - PL - DD -  -  -  - DIS