Data/hora: 14/06/2024 05:58:16

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	04/06/1997	
Data da última atualização: 	04/06/1997	
Autoria: 	DEVAUX, P. KILIAN, A.; KLEINHOFS, A.	
Afiliação: 	Washigton State University, Pullman-USA.	
Título: 	Anther culture and Hordeum bulbosum-derived barleydoubled haploids: mutations and methylation.	
Ano de publicação: 	1993	
Fonte/Imprenta: 	Mol Gen Genet, v. 241, p. 674-679, 1993.	
Idioma: 	Inglês	
Conteúdo: 	Anther culture and Hordeum bulbosum-derived doubled haploid (DH) lines of barley (Hordeum vulgare L.) were analyzed for RFLP and RAPD polymorphisms. Polymorphisms were not detectd in the anther cultureor H. bulbosum-derived DH lines among 273 RFLP and 89 polymerase chain reaction(PCR)-aplified DNA fragments assayed. It was calculated that base substitution or small deletion/insertion mutations had not been induced among 40l 640 bp screened. Large deletion/insertion mutations were not observed among 33 Mb screened. Polymorphisms were observed when DNA wasdigested with the methylation-sensitive restriction enzymes Hpall and MspI: these RFLPs originated primarily from the culture-derived doubled haploids. The data indicate that heritable DNA methylation changeshad occured during DH production, particularly with the anther culture method.	
Palavras-Chave: 	Doubled haploid; Horedeum bulbosum method; Mutation frequency.	
Thesaurus Nal: 	anther culture; DNA methylation.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (16926-1) - ADC - SP - -- - S11042 -  - S11042 - DIS