| |
|
|
| Registros recuperados : 1 | |
| Registros recuperados : 1 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
25/04/1999 |
|
Data da última atualização: |
25/04/1999 |
|
Autoria: |
REIS, V. M.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JUNIOR, F. B.; BALDANI, J. I.; DOBEREINER, Johanna. |
|
Título: |
Technical approaches to inoculate micropropagated sugar cane plants with Acetobacter diazotrophicus. |
|
Ano de publicação: |
1999 |
|
Fonte/Imprenta: |
Plant and Soil, Dordrecht, v. 206, p. 205-211, 1999. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Micropropagated plantlets of sugar cane were inoculated with the N2-fixing bacterium Acetobacter diazotrophicus. Various modifications on the basic plant culture medium MS were made for the plant/bacteria association. The protocol required the inoculation of the bacteria at the end of the rooting period in a medium without hormones or vitamins, and with the concentration of sugar and mineral nutrients reduced by a factor of 10. Individual plants were inoculated with A. diazotrophicus and maintained under the appropriate light and temperature condition used for micropropagation up to 7 days. The system favored the infection and the establishment of the bacteria within the plant tissue. Bacteria colonized the plant tissue and accumulated in inter-cellular cavities and the region of lateral root emergence and also colonizes the xylem vessels. The inoculated plantlets were subsequently transferred to the aclimatization phase and after 30 days it was possible to isolate the bacteria from plant tissue. This protocol permitted studies of infection and comparison among strains. |
|
Palavras-Chave: |
Acetobacter diazotrophicus; BNF; Cana-de-açúcar; FBN; Fixação biologica de nitrogênio; In vitro; Nitrogen fixing bacteria; Sugar cane. |
|
Thesagro: |
Análise Estatística; Bactéria; Inoculação. |
|
Thesaurus NAL: |
inoculation methods; statistical analysis. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02059naa a2200337 a 4500
001 1621100
005 1999-04-25
008 1999 bl --- 0-- u #d
100 1 $aREIS, V. M.
245 $aTechnical approaches to inoculate micropropagated sugar cane plants with Acetobacter diazotrophicus.
260 $c1999
520 $aMicropropagated plantlets of sugar cane were inoculated with the N2-fixing bacterium Acetobacter diazotrophicus. Various modifications on the basic plant culture medium MS were made for the plant/bacteria association. The protocol required the inoculation of the bacteria at the end of the rooting period in a medium without hormones or vitamins, and with the concentration of sugar and mineral nutrients reduced by a factor of 10. Individual plants were inoculated with A. diazotrophicus and maintained under the appropriate light and temperature condition used for micropropagation up to 7 days. The system favored the infection and the establishment of the bacteria within the plant tissue. Bacteria colonized the plant tissue and accumulated in inter-cellular cavities and the region of lateral root emergence and also colonizes the xylem vessels. The inoculated plantlets were subsequently transferred to the aclimatization phase and after 30 days it was possible to isolate the bacteria from plant tissue. This protocol permitted studies of infection and comparison among strains.
650 $ainoculation methods
650 $astatistical analysis
650 $aAnálise Estatística
650 $aBactéria
650 $aInoculação
653 $aAcetobacter diazotrophicus
653 $aBNF
653 $aCana-de-açúcar
653 $aFBN
653 $aFixação biologica de nitrogênio
653 $aIn vitro
653 $aNitrogen fixing bacteria
653 $aSugar cane
700 1 $aOLIVARES, F. L.
700 1 $aOLIVEIRA, A. L. M. de
700 1 $aREIS JUNIOR, F. B.
700 1 $aBALDANI, J. I.
700 1 $aDOBEREINER, Johanna.
773 $tPlant and Soil, Dordrecht$gv. 206, p. 205-211, 1999.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
