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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
24/06/2013 |
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Data da última atualização: |
07/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DELABONA, P. da S.; PIROTA, R. D. P. B.; CODIMA, C. A.; TREMACOLDI, C. R.; RODRIGUES, A.; FARINAS, C. S. |
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Afiliação: |
PRISCILA DA SILVA DELABONA, CNPDIA/UFSCAR; ROSANGELA DONIZETE PERPETUA BUZON PIROTA, CNPDIA/UFSCAR; CARLA ALOIA CODIMA, CNPDIA/UFSCAR; CELIA REGINA TREMACOLDI, CPATU; ANDRÉ RODRIGUES, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Effect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues: Biomass-degrading enzymes production and characterization. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Industrial Crops and Products, v. 42, p. 236-242, 2013. |
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DOI: |
http://dx.doi.org/10.1016/j.indcrop.2012.05.035 |
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Idioma: |
Inglês |
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Conteúdo: |
Production of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in the acidic pH range, with maximal activities at the range of 50?65 °C, demonstrating the potential of the organism for the production of acidophilic and thermophilic biomass-degrading enzymes. MenosProduction of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in... Mostrar Tudo |
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Palavras-Chave: |
Resíduo agroindustrial. |
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Thesagro: |
Biomassa. |
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Thesaurus Nal: |
Amazonia. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02498naa a2200229 a 4500
001 1960431
005 2022-11-07
008 2013 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.indcrop.2012.05.035$2DOI
100 1 $aDELABONA, P. da S.
245 $aEffect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues$bBiomass-degrading enzymes production and characterization.$h[electronic resource]
260 $c2013
520 $aProduction of biomass-degrading enzymes using inexpensive and readily available agricultural residues as substrates for solid-state fermentation (SSF) can contribute to a broader application of enzymes for the conversion of biomass into biofuels and chemicals. Among the operational parameters that affect SSF process efficiency, moisture content is one of the most important. This work evaluates the effect of initial moisture content on two Aspergillus strains (Aspergillus niger P47C3 and Aspergillus fumigatus P40M2), isolated from the Amazon rainforest and grown under SSF. Analyses were made of the biomass-degrading enzymes produced using different agro-industrial residues as carbon sources (wheat bran, sugar cane bagasse, soybean bran, and orange bagasse). The enzymatic complex produced by a selected strain of A. fumigatus was characterized in terms of optimum pH and temperature, and thermal stability. The most effective carbon sources for multienzyme production during Aspergillus cultivation were wheat and soybean bran, as well as a 1:1 mixture of sugar cane bagasse and wheat bran. Much higher activity values were achieved for β-glucosidase (105.8 IU/g) and xylanase (1055.6 IU/g) when wheat bran with 50% initial moisture content was used as substrate. Under this condition, endoglucanase and total cellulase activity values were 56.6 IU/g and 5.0 FPU/g, respectively. Characterization of the crude enzymatic complex showed that the A. fumigatus P40M2 enzymes were active in the acidic pH range, with maximal activities at the range of 50?65 °C, demonstrating the potential of the organism for the production of acidophilic and thermophilic biomass-degrading enzymes.
650 $aAmazonia
650 $aBiomassa
653 $aResíduo agroindustrial
700 1 $aPIROTA, R. D. P. B.
700 1 $aCODIMA, C. A.
700 1 $aTREMACOLDI, C. R.
700 1 $aRODRIGUES, A.
700 1 $aFARINAS, C. S.
773 $tIndustrial Crops and Products$gv. 42, p. 236-242, 2013.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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| Registros recuperados : 20 | |
| 1. |  | PIROTA, R. D. P. B.; TONELOTTO, M.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Comparison of fpase and xylanase production between fungi isolated from amazon region and aspergillus niger (F12). In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 327. res. 411.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 3. | | PIROTA, R. D. P. B.; TONELOTTO, M.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Produção enzimática por fungos isolados da Região Amazônica, visando aplicação na produção de etanol celulósico. In: SEMINÁRIO BRASILEIRO DE TECNOLOGIA ENZIMÁTICA - ENZITEC, 9., 2010, Rio de Janeiro. Versatilidade e eficiência na inovação sustentável: resumos. Rio de Janeiro: UFRJ, 2010. p. 106.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | PIROTA, R. D. P. B.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Produção de carboximetilcelulase e poligalacturonase sólido pelo fungo Trichoderma polysporum por fermentação em estado sólido, utilizando bagaço de laranja com substrato. In: JORNADA CIENTÍFICA-EMBRAPA SÃO CARLOS, 2009, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação Agropecuária, 2009. p. 11 (Embrapa Pecuária Sudeste. Documentos, 90)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | PIROTA, R. D. P. B.; TONELOTTO, M.; DELABONA, P. da S.; TREMACOLDI, C. R.; FARINAS, C. S. Caracterização de fungos isolados da região Amazônica quanto ao potencial para produção das enzimas envolvidas na conversão da biomassa vegetal. Ciência Rural, Santa Maria, v. 45, n. 9, p. 1606-1612, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Instrumentação. |
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| 10. | | DELABONA, P. da S.; PIROTA, R. D. P. B.; CODIMA, C. A.; TREMACOLDI, C. R.; RODRIGUES, A.; FARINAS, C. S. Effect of initial moisure content on two amazon rainforest aspergilus strains cultivated on agroindustrial residues: biomass-degrading enzymes production and characterization. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 34., 2012, New Orleans. Abstract... Old Lee Highway: Society for Industrial Microbiology & Biotechnology, 2012. Paper 20771.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 11. | | DELABONA, P. da S.; LIMA, D. J.; ROBL, D.; RABELO, S. C.; FARINAS, C. S.; PRADELLA, J. G. da C. Enhanced cellulase production by Trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates. Journal of industrial microbiology & biotechnology, [S. l.], v. 43, n. 5, p. 617-626, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 12. | | DELABONA, P. da S.; PIROTA, R. D. P. B.; CODIMA, C. A.; TREMACOLDI, C. R.; RODRIGUES, A.; FARINAS, C. S. Using amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes. Biomass and Bioenergy,Oxford, v. 37, p. 243-250, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Instrumentação. |
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| 16. | | PIROTA, R. D. P. B.; TONELOTTO, M. DELABONA, P. da S.; FONSECA, R. F.; PAIXÃO, D. A. A.; BALEEIRO, F. C. F.; BERTUCCI NETO, V.; FARINAS, C. S. Enhancing xylanases production by a new Amazon Forest strain of aspergillus oryzae using solid-state fermentation under controlled operation conditions. Industrial Crops and Products, [S. l.], v. 45., p. 465-471, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 17. | | PIROTA, R. D. P. B.; BRAGA, C. M. P.; DELABONA, P. da S.; FONSECA, R. F.; BERTUCCI NETO, V.; FARINAS, C. S. Produção de celulases por uma espécie nova de aspergillus oryzae isolado da região amazônica usando biorreator de coluna instrumentado. In: SEMINÁRIO BRASILEIRO DE TECNOLOGIA ENZIMÁTICA, 10., 2012, Blumenau. Resumos expandidos... Blumenau: FURB, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 18. | | DELABONA, P. da S.; SEIBOTH, B.; BRAGA, C. M. P.; RODRIGUES, G. N.; FARINAS, C. S.; PRADELLA, J. G. da C. Overexpression of XYR1 to increase cellulases production in Trichoderma harzianum. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego. Papers... San Diego: Society for industrial microbiology, 2015. 1 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 19. | | COLUSSI, F.; SERPA, V.; DELABONA, P. da S.; MANZINE, L. R.; VOLTATÓDIO, M. L.; ALVES, R.; MELLO, B. L.; PEREIRA JUNIOR, N.; FARINAS, C. S.; GOLUBEV, A. M.; SANTOS, M. A. M.; POLIKARPOV, I. Purification, and biochemical and biophysical characterization of cellobiohydrolase I from trichoderma harzianum IOC 3844. Journal of microbiology and biotechnology, Kangnam-Gu, v. 21, n. 8, p. 808-817, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 20. | | DELABONA, P. da S.; COTA, J.; HOFFMAM, Z. B.; PAIXÃO, D. A. A.; FARINAS, C. S.; CAIRO, J. P. L. F.; LIMA, D. J.; SQUINA, F. M.; RULLER, R.; PRADELLA, J. G. da C. Understanding the cellulolytic system of trichoderma harzianum P49P11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and alfa-L-arabinufuranosidase. Bioresource Technology, Essex, v. 131, p. 500-507, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 20 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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