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Registro Completo |
Biblioteca(s): |
Embrapa Soja; Embrapa Trigo. |
Data corrente: |
26/02/2021 |
Data da última atualização: |
06/12/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CARPENTIERI-PIPOLO, V.; BARRETO, T. P.; SILVA, D. A. da; ABDELNOOR, R. V.; MARIN, S. R. R.; DEGRASSI, G. |
Afiliação: |
VALERIA CARPENTIERI PIPOLO, CNPT; THALES PEREIRA BARRETO, (2) Syngenta Proteção de Cultivos LTDA. São Paulo, SP. E-mail: thalepbarreto@yahoo.com.br; DAIANA ALVES DA SILVA, (3) Agronomic Institute (IAC) - Center for Grain and Fiber - Zip Code 13075-630 – Campinas, SP –Brazil, E-mail: daiagrouel2002@hotmail.com; RICARDO VILELA ABDELNOOR, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; GIULIANO DEGRASSI, (6) International Centre for Genetic Engineering and Biotechnology (ICGEB), 1Industrial Biotechnology Group, Parque Tecnolo?gico Miguelete, San Marti?n, Buenos Aires, Repu?blica Argentina. E-mail: degrassi@icgeb.org. |
Título: |
Soybean improvement for lipoxygenase-free by simple sequence repeat (SSR) markers selection. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Journal of Botanical Research, v. 3, n.1, 2021. |
Idioma: |
Inglês |
Conteúdo: |
Beany flavor of soybean (Glycine max (L.) Merr.) is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases (LOX1, LOX2 and LOX3) present in mature seeds. The unpleasant flavor restricts human consumption of soybean products. This problem could be solved through genetic elimination of alleles that code these enzymes. Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus, encoding Lox1, Lox2 and Lox3 free. The SSR marker Satt212 confirmed the presence of the homozygous null-allele Lx3 in the cultivar BRS 213, which were used for hybridization with BR 36. Heterozygote F1 hybrid plants and homozygous Lx3 lines in F2 segregating populations were successfully identified. The SSR markers Sat090 and Sat417 were the most effective diagnostic markers among all SSR markers tested. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3,00 and 2,77 cM, respectively. The presence of Lx2 null allele in the F2 segregating populations between BRS 213 and BRS 155 were successfully identified with a selection efficiency of 98% and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality. Index terms: Glycine max, lipoxygenase isozymes, molecular markers, MAS |
Palavras-Chave: |
Lipoxigenase; Lipoxygenase isozymes; Market-assisted selection (MAS); MAS; Molecular markers; Simple sequence repeat (SSR). |
Thesagro: |
Glycine Max; Isoenzima; Marcador Molecular; Soja. |
Thesaurus Nal: |
Genetic markers; Isozymes; Lipoxygenase; Soybeans. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228513/1/Soybean-Improvement-for-lipoxygenase-free-2818-12720-1-PB-2021.pdf
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Marc: |
LEADER 02378naa a2200349 a 4500 001 2130306 005 2021-12-06 008 2021 bl uuuu u00u1 u #d 100 1 $aCARPENTIERI-PIPOLO, V. 245 $aSoybean improvement for lipoxygenase-free by simple sequence repeat (SSR) markers selection.$h[electronic resource] 260 $c2021 520 $aBeany flavor of soybean (Glycine max (L.) Merr.) is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases (LOX1, LOX2 and LOX3) present in mature seeds. The unpleasant flavor restricts human consumption of soybean products. This problem could be solved through genetic elimination of alleles that code these enzymes. Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus, encoding Lox1, Lox2 and Lox3 free. The SSR marker Satt212 confirmed the presence of the homozygous null-allele Lx3 in the cultivar BRS 213, which were used for hybridization with BR 36. Heterozygote F1 hybrid plants and homozygous Lx3 lines in F2 segregating populations were successfully identified. The SSR markers Sat090 and Sat417 were the most effective diagnostic markers among all SSR markers tested. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3,00 and 2,77 cM, respectively. The presence of Lx2 null allele in the F2 segregating populations between BRS 213 and BRS 155 were successfully identified with a selection efficiency of 98% and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality. Index terms: Glycine max, lipoxygenase isozymes, molecular markers, MAS 650 $aGenetic markers 650 $aIsozymes 650 $aLipoxygenase 650 $aSoybeans 650 $aGlycine Max 650 $aIsoenzima 650 $aMarcador Molecular 650 $aSoja 653 $aLipoxigenase 653 $aLipoxygenase isozymes 653 $aMarket-assisted selection (MAS) 653 $aMAS 653 $aMolecular markers 653 $aSimple sequence repeat (SSR) 700 1 $aBARRETO, T. P. 700 1 $aSILVA, D. A. da 700 1 $aABDELNOOR, R. V. 700 1 $aMARIN, S. R. R. 700 1 $aDEGRASSI, G. 773 $tJournal of Botanical Research$gv. 3, n.1, 2021.
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Registro original: |
Embrapa Trigo (CNPT) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
11/03/2009 |
Data da última atualização: |
06/10/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - A |
Autoria: |
BENITO, N. P.; GUIMARÃES, M. de F.; PASINI, A. |
Afiliação: |
Norton Pólo Benito, Embrapa Recursos Genéticos e Biotecnologia; Maria de Fátima Guimarães, UEL; Amarildo Pasini, UEL. |
Título: |
Caracterização de sistemas de manejo em latossolo vermelho utilizando parâmetros biológicos, físicos e químicos. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v.9, n.3, p.473-484, jul./set. 2008. |
Páginas: |
p. 473-483. |
Idioma: |
Português |
Palavras-Chave: |
Macrofauna. |
Thesagro: |
Pastagem; Plantio Direto; Solo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178436/1/SP-19710-ID-31143.pdf
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Marc: |
LEADER 00619naa a2200193 a 4500 001 1190866 005 2022-10-06 008 2008 bl uuuu u00u1 u #d 100 1 $aBENITO, N. P. 245 $aCaracterização de sistemas de manejo em latossolo vermelho utilizando parâmetros biológicos, físicos e químicos.$h[electronic resource] 260 $c2008 300 $ap. 473-483. 650 $aPastagem 650 $aPlantio Direto 650 $aSolo 653 $aMacrofauna 700 1 $aGUIMARÃES, M. de F. 700 1 $aPASINI, A. 773 $tSemina: Ciências Agrárias, Londrina$gv.9, n.3, p.473-484, jul./set. 2008.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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