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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
30/09/2008 |
Data da última atualização: |
03/02/2009 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
PARENTE, A. F.; SILVA-PEREIRA, I.; BALDANI, J. I.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. |
Afiliação: |
Ana Flávia Parente, UnB; Ildinete Silva-Pereira, UnB; José Ivo Baldani, Embrapa Agrobiologia; Victor Hugo da Silva Tibúrcio, UnB; Sônia Nair Báo, UnB; Marlene T. de Souza, UnB. |
Título: |
Construction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria-plant interactions. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Canadian Journal of Microbiology, Ottawa, v. 54, p. 786-790, 2008. |
Idioma: |
Inglês |
Notas: |
Parceria: UnB. |
Conteúdo: |
Collectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique expression system a useful experimental model to study adaptive changes of host-plasmid as well as plasmid-plasmid relationships in a population of cells stressed by the production of a recombinant protein. MenosCollectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique e... Mostrar Tudo |
Palavras-Chave: |
Cana-de-açúcar. |
Thesagro: |
Bacillus Thuringiensis; Controle Biológico; Diatraea Saccharalis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02517naa a2200241 a 4500 001 1598585 005 2009-02-03 008 2008 bl --- 0-- u #d 100 1 $aPARENTE, A. F. 245 $aConstruction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein$btwo potential marker organisms to study bacteria-plant interactions. 260 $c2008 500 $aParceria: UnB. 520 $aCollectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique expression system a useful experimental model to study adaptive changes of host-plasmid as well as plasmid-plasmid relationships in a population of cells stressed by the production of a recombinant protein. 650 $aBacillus Thuringiensis 650 $aControle Biológico 650 $aDiatraea Saccharalis 653 $aCana-de-açúcar 700 1 $aSILVA-PEREIRA, I. 700 1 $aBALDANI, J. I. 700 1 $aTIBÚRCIO, V. H. da S. 700 1 $aBÁO, S. N. 700 1 $aDE SOUZA, M. T. 773 $tCanadian Journal of Microbiology, Ottawa$gv. 54, p. 786-790, 2008.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registros recuperados : 19 | |
3. | | BATISTA, B. C. de C.; MONNERAT, R.; MARTINS, E. S.; DE-SOUZA, M. T. Genetic stability analysis of two selected brazilian B. thuringiensis strains active to Lepidoptera. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 10., 2007, Brasília, DF. Inovar para preservar a vida. Resumos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 250). ID 456.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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7. | | SILVA, T. D. DA; SOUZA, M, T. DE; SOUZA, M. T. DE; CIPRIANO, R. R.; BIZZO, H. R.; DESCHAMPS, C. Essential Oil Variation in Brazilian Varronia curassavica Jacq. in Response to Drying and Edaphoclimatic Conditions. Journal of Agricultural Science, v. 13, n. 8, 2021. 15 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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8. | | ASSIS, J. S. de; RESENDE, S. V.; OLIVEIRA, A. L. P. de; SOUZA, M. T. de B.; PERDIZ, M. L.; QUEIROZ, M. A. de. Avaliação da diversidade genética de populações de melancia (Citrullus lanatus) através de eletroforense de proteínas. Dados preliminares. In: ENCONTRO DE GENÉTICA DO NORDESTE, 14.,1999, Recife. Resumos... Recife: SBG-Regional do Nordeste, 1999. p. 40.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Semiárido. |
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9. | | MARTUSCELLO, J. A.; SOUZA, M. W. M.; BRITO, G. F. de; SOUZA, M. T. de C.; JANK, L.; BRAZ, T. G. dos S. Características Morfogênicas e Estruturais em Cultivares de Panicum maximum no Agreste Alagoano. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científicos na zootecnia brasileira de vanguarda: anais. Salvador: SBZ: UFBA, 2010. 3 p. R4108Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Corte. |
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10. | | PARENTE, A. F.; BALDANI, J. I.; SILVA-PEREIRA, I.; TIBÚRCIO, V. H. da S.; BÁO, S. M.; DE-SOUZA, M.T. GFP expression in the wild-type B. thuringiensis S76: a putative marker for detection of active strains in the environment. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 10., 2007, Brasília, DF. Inovar para preservar a vida. Resumos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 1 p. 1 CD-ROM. SICONBIOL. Parceria: UNBTipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Agrobiologia. |
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11. | | PARENTE, A. F.; BALDANI, J. I.; PEREIRA, I. S.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. GFP expression in wild-type B. thuringiensis strain active to Lepidoptera. In: MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 40th., INTERNATIONAL FORUM ON ENTOMOPATHOGENIC NEMATODES AND SYMBIOTIC BACTERIA, 1st., 2007, Quebec City. Anais... Quebec: Society for Invertebrate Pathology, 2007. p. 59. Parceria: UnB.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agrobiologia. |
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12. | | PARENTE, A. F.; SILVA-PEREIRA, I.; BALDANI, J. I.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. Construction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria-plant interactions. Canadian Journal of Microbiology, Ottawa, v. 54, p. 786-790, 2008. Parceria: UnB.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Agrobiologia. |
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13. | | DIAS, J. M. C. S.; GIUSTI-DA COSTA, J.; TOSTES, A. N.; ALBINO, S. B.; COSTA, D. C.; MELLO, G. M.; DE SOUZA, M. T. Desenvolvimento do processo descontinuo de produção de bacillus thuringiensis S93 efetivo contra a lagarta do cartucho. In: TALENTO Estudantil da Embrapa Recursos Genéticos e Biotecnologia 1998: resumo dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 1998. p. 68. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 34).Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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14. | | SOUZA, M. W. M.; BRITO, G. F. de; SOUZA, M. T. de C.; MARTUSCELO, J. A.; JANK, L.; CUNHA, D. de N. F. V. da. Produção de massa seca em cultivares de Panicum maximum no Agreste Alagoano. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científicos na zootecnia brasileira de vanguarda: anais. Salvador: SBZ: UFBA, 2010. 3 p. R5511Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Corte. |
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16. | | LINS, P. M. P.; RIBEIRO, R. C.; LEMOS, W. de P.; MÜLLER, A. A.; AZEVEDO, R. de; SOUZA, M. T. de S. Estimativa da sobrevivência de adultos de Homalinotus Depressus L. (Col., Curculionidae) submetidos ao insecticida Clorpirifós em laboratório. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: resumos. Viçosa: UFV, 2008. Resumo 1475-2.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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17. | | LIMA, G. M. S.; AGUIAR, R. W. S.; CORRÊA, R. F. T.; MARTINS, E. S.; GOMES, A. C. M.; NAGATA, T.; DE-SOUZA, M. T.; MONNERAT, R, G.; RIBEIRO, B. M. Cry2A toxins from Bacillus thuringiensis expressed in insect cells are toxic to two lepidopteran insects. World Journal of Microbiology and Biotechnology, v.24, p. 2941-2948, 2008.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | MELO, L. H. V. de; GALVÃO, P. G.; GITAHY, P. de M.; DE SOUZA, M. T.; SILVA, F. R.; VIDAL, M. S.; SIMÕES-ARAÚJO, J. L.; BALDANI, J. I. Sequenciamento preliminar de um plasmídeo de Bacillus thuringiensis subsp. Kurstaki s76. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24.; SIMPÓSIO BRASILEIRO DE MICROBACTÉRIAS, 12.; SIMPÓSIO DE COLEÇÕES DE CULTURA, 2.; ENCONTRO DE ENSINO EM MICROBIOLOGIA, 4., 2007, Brasília, DF. Anais. {São Paulo}: SBM, 2007. 1 p. 1 CD-ROM. Parceria: não temTipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Agrobiologia. |
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19. | | PORSANI, M. V.; POITEVIN, C. G.; TRALAMAZZA, S. M.; SOUZA, M. T. de; SOUZA, M. T. de; SILVA, E. D. B. da; ZAWADNEAK, M. A. C.; PIMENTEL, I. C.; MELO, I. S. de. Streptomyces spp. isolated from marine and caatinga biomes in Brazil for the biological control of Duponchelia fovealis. Neotropical Entomology, v. 51, n. 2, p. 299-310, 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 19 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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