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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/11/2022 |
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Data da última atualização: |
13/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SERRA, L. A.; CRUZ, R. G. da S.; GUTIERREZ, D. M. R.; CRUZ, A. J. G.; CANIZARES, C. A. T.; CHEN, X.; MOSIER, N.; THOMPSON, D.; ASTON, J.; DOOLEY, J.; SHARMA, P.; DE MARCO, J. L.; ALMEIDA, J. R. M. de; ERK, K.; XIMENES, E.; LADISCH, M. R. |
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Afiliação: |
LUANA ASSIS SERRA, PURDUE UNIVERSITY; ROSINEIDE GOMES DA SILVA CRUZ, PURDUE UNIVERSITY; DIANA M. R. GUTIERREZ, PURDUE UNIVERSITY; ANTONIO JOSE GONÇALVES CRUZ, PURDUE UNIVERSITY; CARLOS ALBERTO TORRES CANIZARES, PURDUE UNIVERSITY; XUELI CHEN, PURDUE UNIVERSITY; NATHAN MOSIER, PURDUE UNIVERSITY; DAVID THOMPSON, IDAHO NATIONAL LABORATORY; JOHN ASTON, IDAHO NATIONAL LABORATORY; JAMES DOOLEY, FOREST CONCEPTS LLC; PANKAJ SHARMA, PURDUE UNIVERSITY; JANICE LISBOA DE MARCO, UNIVERSITY OF BRASÍLIA; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; KENDRA ERK, PURDUE UNIVERSITY/ SCHOOL OF MATERIALS ENGINEERING; EDUARDO XIMENES, PURDUE UNIVERSITY; MICHAEL R. LADISCH, PURDUE UNIVERSITY. |
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Título: |
Screening method for Enzyme-based liquefaction of corn stover pellets at high solids. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Bioresource Technology, v. 363, 127999, Nov., 2022. |
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DOI: |
https://doi.org/10.1016/j.biortech.2022.127999 |
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Idioma: |
Inglês |
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Conteúdo: |
Liquefaction of high solid loadings of unpretreated corn stover pellets has been demonstrated with rheology of the resulting slurries enabling mixing and movement within biorefinery bioreactors. However, some forms of pelleted stover do not readily liquefy, so it is important to screen out lots of unsuitable pellets before processing is initiated. This work reports a laboratory assay that rapidly assesses whether pellets have the potential for enzyme-based liquefaction at high solids loadings. Twenty-eight pelleted corn stover (harvested at the same time and location) were analyzed using 20 mL enzyme solutions (3 FPU cellulase/ g biomass) at 30 % w/v solids loading. Imaging together with measurement of reducing sugars were performed over 24-hours. Some samples formed concentrated slurries of 300 mg/mL (dry basis) in the small-scale assay, which was later confirmed in an agitated bioreactor. Also, the laboratory assay showed potential for optimizing enzyme formulations that could be employed for slurry formation. |
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Palavras-Chave: |
Biorefinery; Laboratory assay; Lignocellulosic biomass. |
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Thesaurus Nal: |
Corn stover; Enzymes; Lignocellulosic wastes. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02119naa a2200385 a 4500
001 2148655
005 2023-02-13
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.biortech.2022.127999$2DOI
100 1 $aSERRA, L. A.
245 $aScreening method for Enzyme-based liquefaction of corn stover pellets at high solids.$h[electronic resource]
260 $c2022
520 $aLiquefaction of high solid loadings of unpretreated corn stover pellets has been demonstrated with rheology of the resulting slurries enabling mixing and movement within biorefinery bioreactors. However, some forms of pelleted stover do not readily liquefy, so it is important to screen out lots of unsuitable pellets before processing is initiated. This work reports a laboratory assay that rapidly assesses whether pellets have the potential for enzyme-based liquefaction at high solids loadings. Twenty-eight pelleted corn stover (harvested at the same time and location) were analyzed using 20 mL enzyme solutions (3 FPU cellulase/ g biomass) at 30 % w/v solids loading. Imaging together with measurement of reducing sugars were performed over 24-hours. Some samples formed concentrated slurries of 300 mg/mL (dry basis) in the small-scale assay, which was later confirmed in an agitated bioreactor. Also, the laboratory assay showed potential for optimizing enzyme formulations that could be employed for slurry formation.
650 $aCorn stover
650 $aEnzymes
650 $aLignocellulosic wastes
653 $aBiorefinery
653 $aLaboratory assay
653 $aLignocellulosic biomass
700 1 $aCRUZ, R. G. da S.
700 1 $aGUTIERREZ, D. M. R.
700 1 $aCRUZ, A. J. G.
700 1 $aCANIZARES, C. A. T.
700 1 $aCHEN, X.
700 1 $aMOSIER, N.
700 1 $aTHOMPSON, D.
700 1 $aASTON, J.
700 1 $aDOOLEY, J.
700 1 $aSHARMA, P.
700 1 $aDE MARCO, J. L.
700 1 $aALMEIDA, J. R. M. de
700 1 $aERK, K.
700 1 $aXIMENES, E.
700 1 $aLADISCH, M. R.
773 $tBioresource Technology$gv. 363, 127999, Nov., 2022.
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Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 11 | |
| 4. |  | MARTINS, P. A.; PACHECO, T. F.; NAKAI, D. K.; DE MARCO, J. L.; SALUM, T. F. C. Uso de lipases bacterianas para a síntese de biodiesel etílico. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 28| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | MARTINS, P. A.; PACHECO, T. F.; CAMARGO, B. R. de; DE MARCO, J. L.; SALUM, T. F. C. Lipases de Burkholderia gladioli CNPAE 99 (579): caracterização do efeito de íons, surfactantes e solventes e determinação de parâmetros cinéticos. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 165-172 il.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | DE MARCO, J. L.; AZEVEDO, A. M. C. de; LIMA, L. H. C.; CAMPOS, L.; FELIX, C. R. Production of hydrolases by Trichoderma sp isolates showing antagonistic activity against Sclerotium rolfsii, Rhizoctonia solani, and Crinipellis perniciosa. In: SEMINARIO BRASILEIRO DE TECNOLOGIA ENZIMATICA, 5., 2002, Brasilia. ENZITEC 2002. Brasilia: UnB, 2002. p. 37.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | SANTOS, C. M. B.; COELHO, G. M.; CUPERTINO, F. B.; PIOVANI, C. P. P.; CASCARDO, J. C. M.; LOGUERCIO, L. L.; FÉLIX, C. R.; DE MARCO, J. L.; CARVALHO, L. J. C. B.; POMELLA, A. W. V.; COSTA, M. G. C. Proteômica de fungos filamentosos: mapeamento inicial do proteoma extracelular do agente de controle biológico Trichoderma stromaticum. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 1082.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | SERRA, L. A.; CRUZ, R. G. da S.; GUTIERREZ, D. M. R.; CRUZ, A. J. G.; CANIZARES, C. A. T.; CHEN, X.; MOSIER, N.; THOMPSON, D.; ASTON, J.; DOOLEY, J.; SHARMA, P.; DE MARCO, J. L.; ALMEIDA, J. R. M. de; ERK, K.; XIMENES, E.; LADISCH, M. R. Screening method for Enzyme-based liquefaction of corn stover pellets at high solids. Bioresource Technology, v. 363, 127999, Nov., 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 11 | |
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