| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
05/10/2011 |
|
Data da última atualização: |
10/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E. |
|
Afiliação: |
INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, SAPC; UNIVERSIDADE FEDERAL DO PARANÁ; UNIVERSIDADE FEDERAL DO PARANÁ. |
|
Título: |
Drought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
Environmental and Experimental Botany, v.72, n. 2, p. 242-250, 2011. |
|
Idioma: |
Inglês |
|
Conteúdo: |
In this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was more active in control than in transgenic plants under irrigated condition and both stress levels. Our results suggest that transgenic plants were able to cope with water deficit better than non-transformed controls since the high endogenous proline level acted not only by mediating osmotic adjustment, but also by contributing to gas exchange parameters and ameliorating deleterious effects of drought-induced oxidative stress. MenosIn this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was m... Mostrar Tudo |
|
Palavras-Chave: |
Citrus rootstock; Osmotic adjustment. |
|
Thesaurus Nal: |
Drought tolerance; Oxidative stress; Proline. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42905/1/Drought-tolerance-and-antioxidant.pdf
|
|
Marc: |
LEADER 02726naa a2200253 a 4500
001 1902435
005 2024-01-10
008 2011 bl uuuu u00u1 u #d
100 1 $aCAMPOS, M. K. F. de
245 $aDrought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline.$h[electronic resource]
260 $c2011
520 $aIn this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was more active in control than in transgenic plants under irrigated condition and both stress levels. Our results suggest that transgenic plants were able to cope with water deficit better than non-transformed controls since the high endogenous proline level acted not only by mediating osmotic adjustment, but also by contributing to gas exchange parameters and ameliorating deleterious effects of drought-induced oxidative stress.
650 $aDrought tolerance
650 $aOxidative stress
650 $aProline
653 $aCitrus rootstock
653 $aOsmotic adjustment
700 1 $aCARVALHO, K. de
700 1 $aSOUZA, F. S. de
700 1 $aMARUR, C. J.
700 1 $aPEREIRA, L. F. P.
700 1 $aBESPALHOK FILHO, J. C.
700 1 $aVIEIRA, L. G. E.
773 $tEnvironmental and Experimental Botany$gv.72, n. 2, p. 242-250, 2011.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 9 | |
| 2. |  | LIMA, J. L. S. de; CAVALCANTI, N. de B.; LIMA, E. R. de; CARVALHO, K. M. de. Levantamento fitoecologico do municipio de Petrolina, PE. In: CONGRESSO NACIONAL DE BOTANICA, 47., 1996, Nova Friburgo, RJ. Resumos... Nova Friburgo: SBB, 1996. p. 190.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
|    |
| 3. | | CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E. Drought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline. Environmental and Experimental Botany, v.72, n. 2, p. 242-250, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
| |
| 4. | | LIMA, J. L. S. de; CAVALCANTI, N. de B.; LIMA, E. R. de; CARVALHO, K. M. de; ORESOTU, B. A.; OLIVEIRA, C. A. V. Levantamento fitoecologico do municipio de Afranio- PE. In: CONGRESSO NACIONAL DE BOTANICA, 48., 1997, Crato. Resumos... Fortaleza: BNB, 1997. p. 230.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
| |
| 5. | | LIMA, J. L. S. de; CAVALCANTI, N. de B.; LIMA, E. R. de; CARVALHO, K. M. de; ORESOTU, B. A.; OLIVEIRA, C. A. V. Levantamento fitoecologico do municipio de Ouricuri- PE. In: CONGRESSO NACIONAL DE BOTANICA, 48., 1997, Crato. Resumos... Fortaleza: BNB, 1997. p. 230| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
| |
| 6. | | NORONHA, A. C. da S.; ISHIDA, A. K. N.; MENDONÇA, C. L. G. de; CARVALHO, K. B. de A.; FERREIRA, C. T.; NASCIMENTO, A. S. do. Monitoramento de Diaphorina citri (Hemiptera: Liviidae) em pomares de citros no estado do Pará. In: CONFERÊNCIA NACIONAL DE DEFESA AGROPECUÁRIA, 4., 2013, Belém, PA. Defesa agropecuária e sustentabilidade. Belém, PA: SBDA, 2014. p. 194-195.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 7. | | SILVA, L. F. da; LOPES, O. F.; MENDONÇA, V. R. de; CARVALHO, K. T. G.; LONGO, E.; RIBEIRO, C.; MASTELARO, V. R. An understanding of the photocatalytic properties and pollutant degradation mechanism of SrTiO3 nanoparticles. [3 é subscrito]. Photochemistry and Photobiology, [S. l.], v. 92, p. 371-378, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Instrumentação. |
|  |
| 8. | | SILVA, E. H. F. M. da; ANTOLIN, L. A. S.; ZANON, A. J.; ANDRADE JUNIOR, A. S. de; SOUZA, H. A. de; CARVALHO, K. dos S.; VIEIRA JUNIOR, N. A.; MARIN, F. R. Impact assessment of soybean yield and water productivity in Brazil due to climate change. European Journal of Agronomy, v. 129, 126329, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio-Norte. |
| |
| 9. | | COSTA, R. P. S.; DE CARVALHO, K.; RINCÃO, M. P.; NOMURA, R. B. G.; AVELINO, B. B.; LOPES, I. de O. N.; ABDELNOOR, R. V.; DE CARVALHO, M. C. C. G.; MARCELINO-GUIMARÃES, F. C. Validation of a system for functional gene analysis in P. pachyrhizi by ectopic application of dsRNA. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
| |
| Registros recuperados : 9 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
