Data/hora: 18/06/2024 13:22:40

Biblioteca(s): | Ebooks.
Data corrente: | 15/08/2008
Data da última atualização: | 16/06/2011
Autoria: | PERIASAMY, A.; DAY, R. N.
Afiliação: | Ammasi Periasamy; Richard N. Day.
Título: | Molecular imaging FRET microscopy and spectroscopy
Ano de publicação: | 2005
Fonte/Imprenta: | New York : Published for the American Physiological Society by Oxford University Press, 2005.
Páginas: | xv, 312 p.
Descrição Física: | ill. (some col.) ;26 cm.
Série: | The American Physiological Society methods in physiology series
ISBN: | 9780195177206
Idioma: | Inglês
Conteúdo: | Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy - Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization.The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.
Palavras-Chave: | Fluorescence Resonance Energy Transfer; Fluorescencemethods; methods; Microscopie de fluorescence; Spectrometry; Spectroscopie de fluorescence.
Thesaurus Nal: | fluorescence.
Categoria do assunto: | --
URL: | http://www.sciencedirect.com/science/book/9780195177206
Registro original: | Ebooks (Ebooks)