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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/01/2011 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RODRIGUES, A. S.; BARBOSA, C. de J.; SOARES FILHO, W. dos S.; FREITAS-ASTUA, J. de. |
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Afiliação: |
Almir Santos Rodrigues, UFRB; CRISTIANE DE JESUS BARBOSA, CNPMF; WALTER DOS SANTOS SOARES FILHO, CNPMF; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Citrus rootstock hybrids tolerants to citrus tristeza virus and Bahia bark scaling disease. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
106_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Citrus tristeza virus (CTV) and Bahia bark scaling disease (BBS) are the important viral disease of citrus in the Bahia state in Brazil. Evaluation of germsplasm bank and new hybrids for incidence and severity of these diseases is essential for developing new varieties. In this work 160 hybrids (grafted and un-grafted rootstocks) generated by the Citrus breeding program of Embrapa Cassava & Tropical Fruits were evaluated for CTV and BBS incidence under natural infection in the Recôncavo Southern of Bahia state. Severity of stem pitting of CTV and BBS symptoms were evaluated by using a rating scale. It was used serological method of ELISA (indirect method) to detect the presence of CTV. The samples were made of bark of young branches, diluted in carbonate buffer at 1:100, and for detection, it was used a polyclonal antiserum diluted 1:10,000 in PBS-Tween 0.05% adding 2% PVP and 0.2% egg albumin. The negative control was obtained from the bark of Poncirus trifoliata and the positive control from Mexican lime (Citrus aurantifolia Christm. Swing.) The confirmation of the results was performed by RT-PCR using two pairs of primers specific for genes p23 and p20. Hybrids immunes, tolerant and intolerant to CTV were identified. However, most of hybrids showed symptoms of BBS, mainly those generated using tangerine as a parent. |
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Palavras-Chave: |
Bahia Bark Scaling; Germsplasm; Plant disease. |
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Thesagro: |
Doença de Planta; Fruta Cítrica; Vírus. |
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Thesaurus NAL: |
Citrus tristeza virus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02222nam a2200241 a 4500
001 1872736
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, A. S.
245 $aCitrus rootstock hybrids tolerants to citrus tristeza virus and Bahia bark scaling disease.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a106_PS2 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aCitrus tristeza virus (CTV) and Bahia bark scaling disease (BBS) are the important viral disease of citrus in the Bahia state in Brazil. Evaluation of germsplasm bank and new hybrids for incidence and severity of these diseases is essential for developing new varieties. In this work 160 hybrids (grafted and un-grafted rootstocks) generated by the Citrus breeding program of Embrapa Cassava & Tropical Fruits were evaluated for CTV and BBS incidence under natural infection in the Recôncavo Southern of Bahia state. Severity of stem pitting of CTV and BBS symptoms were evaluated by using a rating scale. It was used serological method of ELISA (indirect method) to detect the presence of CTV. The samples were made of bark of young branches, diluted in carbonate buffer at 1:100, and for detection, it was used a polyclonal antiserum diluted 1:10,000 in PBS-Tween 0.05% adding 2% PVP and 0.2% egg albumin. The negative control was obtained from the bark of Poncirus trifoliata and the positive control from Mexican lime (Citrus aurantifolia Christm. Swing.) The confirmation of the results was performed by RT-PCR using two pairs of primers specific for genes p23 and p20. Hybrids immunes, tolerant and intolerant to CTV were identified. However, most of hybrids showed symptoms of BBS, mainly those generated using tangerine as a parent.
650 $aCitrus tristeza virus
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aVírus
653 $aBahia Bark Scaling
653 $aGermsplasm
653 $aPlant disease
700 1 $aBARBOSA, C. de J.
700 1 $aSOARES FILHO, W. dos S.
700 1 $aFREITAS-ASTUA, J. de
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Embrapa Mandioca e Fruticultura (CNPMF) |
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