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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
11/12/2020 |
Data da última atualização: |
11/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L. |
Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, Cenargen. |
Título: |
Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Theriogenology, Vv. 160, p. 134-141, 2021. |
Páginas: |
p. 134-141 |
ISSN: |
0093-691X |
Idioma: |
Português |
Conteúdo: |
In this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. MenosIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased ... Mostrar Tudo |
Palavras-Chave: |
Apoptose celular; Criotolerância; Fertilização; Melatonina; Vitrificação. |
Thesagro: |
Cultura de Embrião; Embrião. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02843naa a2200349 a 4500 001 2127986 005 2020-12-11 008 2021 bl uuuu u00u1 u #d 022 $a0093-691X 100 1 $aMARQUES, T. C. 245 $aBlastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos.$h[electronic resource] 260 $c2021 300 $ap. 134-141 520 $aIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. 650 $aCultura de Embrião 650 $aEmbrião 653 $aApoptose celular 653 $aCriotolerância 653 $aFertilização 653 $aMelatonina 653 $aVitrificação 700 1 $aSANTOS, E. C. da S. 700 1 $aDIESEL, T. O. 700 1 $aMARTINS, C. F. 700 1 $aCUMPA, H. C. B. 700 1 $aLEME, L. de O. 700 1 $aDODE, M. A. N. 700 1 $aALVES, B. G. 700 1 $aCOSTA, F. P. H. 700 1 $aOLIVEIRA, E. B. 700 1 $aGAMBARINI, M. L. 773 $tTheriogenology, Vv. 160, p. 134-141, 2021.
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Embrapa Cerrados (CPAC) |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
17/01/2008 |
Data da última atualização: |
18/04/2024 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SANTOS, J. C. L. dos; LOBATO, M. P.; TRINDADE, N. S.; FERREIRA, I. C. S.; SOUZA FILHO, A. P. S.; ARRUDA, M. S. P.; GUILHON, G. M. S. P.; ARRUDA, A. C.; SANTOS, L. S. |
Afiliação: |
Josiane C. L. dos Santos, UFPA; Málison P. Lobato, UFPA; Nilson S. Trindade, UFPA; Isabel C. S. Ferreira, UFPA; ANTONIO PEDRO DA SILVA SOUZA FILHO, CPATU; Mara S. P. Arruda, UFPA; Giselle M. S. P. Guilhon, UFPA; Alberto C. Arruda, UFPA. |
Título: |
Atividade potencialmente alelopática de óleo de sementes de Neem (Azadirachta indica). |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 29., 2006, Águas de Lindóia. Anais... Águas de Lindóia: Sociedade Brasileira de Química, 2006. |
Idioma: |
Português |
Palavras-Chave: |
Neem. |
Thesagro: |
Alelopatia; Óleo; Semente. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82338/1/T0489-2.pdf
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Marc: |
LEADER 00817nam a2200241 a 4500 001 1391401 005 2024-04-18 008 2006 bl uuuu u00u1 u #d 100 1 $aSANTOS, J. C. L. dos 245 $aAtividade potencialmente alelopática de óleo de sementes de Neem (Azadirachta indica).$h[electronic resource] 260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 29., 2006, Águas de Lindóia. Anais... Águas de Lindóia: Sociedade Brasileira de Química$c2006 650 $aAlelopatia 650 $aÓleo 650 $aSemente 653 $aNeem 700 1 $aLOBATO, M. P. 700 1 $aTRINDADE, N. S. 700 1 $aFERREIRA, I. C. S. 700 1 $aSOUZA FILHO, A. P. S. 700 1 $aARRUDA, M. S. P. 700 1 $aGUILHON, G. M. S. P. 700 1 $aARRUDA, A. C. 700 1 $aSANTOS, L. S.
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