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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
08/01/2024 |
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Data da última atualização: |
08/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PRADO, G. S.; ROCHA, D. C.; SANTOS, L. N. dos; CONTILIANI, D. F.; NOBILE, P. M.; MARTINATI-SCHENK, J. C.; PADILHA, L.; MALUF, M. P.; LUBINI, G.; PEREIRA, T. C.; MONTEIRO-VITORELLO, C. B.; CRESTE, S.; BOSCARIOL-CAMARGO, R. L.; TAKITA, M. A.; CRISTOFANI-YALY, M.; SOUZA, A. A. de. |
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Afiliação: |
GUILHERME SOUZA PRADO, INSTITUTO AGRONÔMICO; DHIÔVANNA CORRÊIA ROCHA, UNIVERSIDADE ESTADUAL DE CAMPINAS; LUCAS NASCIMENTO DOS SANTOS, INSTITUTO AGRONÔMICO; DANYEL FERNANDES CONTILIANI, INSTITUTO AGRONÔMICO; PAULA MACEDO NOBILE, INSTITUTO AGRONÔMICO; JULIANA CAMARGO MARTINATI-SCHENK, INSTITUTO AGRONÔMICO; LILIAN PADILHA, CNPCa; MIRIAN PEREZ MALUF, CNPCa; GREICE LUBINI, INSTITUTO AGRONÔMICO; TIAGO CAMPOS PEREIRA, UNIVERSIDADE DE SÃO PAULO; CLAUDIA BARROS MONTEIRO-VITORELLO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SILVANA CRESTE, INSTITUTO AGRONÔMICO; RAQUEL LUCIANA BOSCARIOL-CAMARGO, INSTITUTO AGRONÔMICO; MARCO AURÉLIO TAKITA, INSTITUTO AGRONÔMICO; MARIÂNGELA CRISTOFANI-YALY, INSTITUTO AGRONÔMICO; ALESSANDRA ALVES DE SOUZA, INSTITUTO AGRONÔMICO. |
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Título: |
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 14, article 1331258, 2023. |
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DOI: |
https://doi.org/10.3389/fpls.2023.1331258 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars. MenosGene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its applica... Mostrar Tudo |
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Thesaurus Nal: |
Citrus; Coffee beans; Genome; Perennials; Sugarcane. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160555/1/CRISPR-technology-towards.pdf
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Marc: |
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024 7 $ahttps://doi.org/10.3389/fpls.2023.1331258$2DOI
100 1 $aPRADO, G. S.
245 $aCRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.$h[electronic resource]
260 $c2023
520 $aGene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars.
650 $aCitrus
650 $aCoffee beans
650 $aGenome
650 $aPerennials
650 $aSugarcane
700 1 $aROCHA, D. C.
700 1 $aSANTOS, L. N. dos
700 1 $aCONTILIANI, D. F.
700 1 $aNOBILE, P. M.
700 1 $aMARTINATI-SCHENK, J. C.
700 1 $aPADILHA, L.
700 1 $aMALUF, M. P.
700 1 $aLUBINI, G.
700 1 $aPEREIRA, T. C.
700 1 $aMONTEIRO-VITORELLO, C. B.
700 1 $aCRESTE, S.
700 1 $aBOSCARIOL-CAMARGO, R. L.
700 1 $aTAKITA, M. A.
700 1 $aCRISTOFANI-YALY, M.
700 1 $aSOUZA, A. A. de
773 $tFrontiers in Plant Science$gv. 14, article 1331258, 2023.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/11/2016 |
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Data da última atualização: |
17/11/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BUSSINGUER, A. P.; NIVA, C. C.; SANTOS, D. P. dos; GATTO, A.; MARCHAO, R. L.; PULROLNIK, K. |
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Afiliação: |
ANGELA PEREIRA BUSSINGUER, UNB; CINTIA CARLA NIVA, CPAC; DJAVAN PINHEIRO DOS SANTOS, UFG; ALCIDES GATTO, UNB; ROBELIO LEANDRO MARCHAO, CPAC; KARINA PULROLNIK, CPAC. |
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Título: |
Caracterização da macrofauna edáfica em sistemas silvipastoris no Cerrado. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. FertBio 2016. |
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Páginas: |
p. 1162. |
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Idioma: |
Português |
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Palavras-Chave: |
ILPF; Integração lavoura pecuária floresta; Macrofauna; Sistema silvipastoril. |
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Thesagro: |
Biodiversidade; Cerrado. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/150119/1/CPAC-2.pdf
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Marc: |
LEADER 00986nam a2200241 a 4500
001 2056504
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008 2016 bl uuuu u00u1 u #d
100 1 $aBUSSINGUER, A. P.
245 $aCaracterização da macrofauna edáfica em sistemas silvipastoris no Cerrado.
260 $aIn: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. FertBio 2016.$c2016
300 $ap. 1162.
650 $aBiodiversidade
650 $aCerrado
653 $aILPF
653 $aIntegração lavoura pecuária floresta
653 $aMacrofauna
653 $aSistema silvipastoril
700 1 $aNIVA, C. C.
700 1 $aSANTOS, D. P. dos
700 1 $aGATTO, A.
700 1 $aMARCHAO, R. L.
700 1 $aPULROLNIK, K.
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Embrapa Cerrados (CPAC) |
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