Data/hora: 15/06/2024 01:12:53

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 12/05/1995
Data da última atualização: | 12/05/1995
Autoria: | LAVI, U.; AKKAYA, M.; BHAGWAT, A.; LAHAV, E.; CREGAN, P. B.
Afiliação: | Instituto of horticulture; United states departament of agriculture.
Título: | Methodology of generation and characteristics of simple sequence repeat DNA markers in avocado (Persea americana M.).
Ano de publicação: | 1994
Fonte/Imprenta: | Euphytica v.80, n.171-177, 1994.
ISSN: | 0014-2336
Idioma: | Inglês
Conteúdo: | Generation of simple sequence repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequence repeats (SSR) and to generate polymerase chain reaction (PCR) primers based on the sequence flanking the simple sequence repeat. Twenty six different pairs of primers which yield a PCR product in the initial screening were synthesized. The SSR A1E11 was found to have eleven alleles while A3F8 has eight  alleles. The SSRs  in avocado were found to be inherited in a Mendelian fashion.
Palavras-Chave: | Avocado; Genectics; Microsatellites; PCR; SSR.
Thesagro: | Persea Americana.
Thesaurus Nal: | breeding.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (2582-1) - ADC - AP - -- - 575.05 -  - 575.05 - DIS