Data/hora: 12/06/2024 01:28:49

Biblioteca(s): 	Embrapa Suínos e Aves.	
Data corrente: 	10/11/2020	
Data da última atualização: 	10/11/2020	
Autoria: 	OLIVEIRA, H. C. de; IBELLI, A. M. G.; GUIMARÃES, S. E. F.; CANTAO, M. E.; PEIXOTO, J. de O.; COUTINHO. L. L.; LEDUR, M. C.	
Afiliação: 	HANIEL CEDRAZ DE OLIVEIRA, UFL; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; SIMONE ELIZA FACIONI GUIMARÃES, UFL; MAURICIO EGIDIO CANTAO, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; LUIZ LEHMANN COUTINHO, USP; MONICA CORREA LEDUR, CNPSA.	
Título: 	RNA-seq reveals downregulated osteochondral genes potentially related totibia bacterial chondronecrosis with osteomyelitis in broilers.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	BMC Genetics, v. 21, n. 58, 2020.	
DOI: 	https://doi.org/10.1186/s12863-020-00862-2	
Idioma: 	Inglês	
Conteúdo: 	Abstract Background: Bacterial chondronecrosis with osteomyelitis (BCO) develops in the growth plate (GP) of the proximal femur and tibia and is initiated by damage to the less mineralized chondrocytes followed by colonization of opportunistic bacteria. This condition affects approximately 1% of all birds housed, being considered one of the major causes of lameness in fast growing broilers. Although several studies have been previously performed aiming to understand its pathogenesis, the molecular mechanisms involved with BCO remains to be elucidated. Therefore, this study aimed to generate a profile of global differential gene expression involved with BCO in the tibia of commercial broilers, through RNA sequencing analysis to identity genes and molecular pathways involved with BCO in chickens. Results: Our data showed 192 differentially expressed (DE) genes: 63 upregulated and 129 downregulated in the GP of the tibia proximal epiphysis of BCO-affected broilers. Using all DE genes, six Biological Processes (BP) were associated with bone development (connective tissue development, cartilage development, skeletal system development, organ morphogenesis, system development and skeletal system morphogenesis). The analyses of the upregulated genes did not indicate any significant BP (FDR < 0.05). However, with the downregulated genes, the same BP were identified when using all DE genes in the analysis, with a total of 26 coding genes explaining BCO in the tibia: ACAN, ALDH1A2, CDH7, CHAD, CHADL, COL11A1, COMP, CSGALNACT1, CYR61, FRZB, GAL3ST1, HAPLN1, IHH, KIF26B, LECT1, LPPR1, PDE6B, RBP4A, SERINC5, SFRP1, SOX8, SOX9, TENM2, THBS1, UCHL1 and WFIKKN2. In addition, seven transcription factors were also associated to BCO: NFATC2, MAFB, HIF1A-ARNT, EWSR1-FLI1, NFIC, TCF3 and NF-KAPPAB. Conclusions: Our data show that osteochondral downregulated genes are potential molecular causes of BCO in broilers, and the bacterial process seems to be, in fact, a secondary condition. Sixteen genes responsible for bone and cartilage formation were downregulated in BCO-affected broilers being strong candidate genes to trigger this disorder.	
Palavras-Chave: 	BCO; RNA-Seq.	
Thesagro: 	Cartilagem; Claudicação; Frango de Corte; Genética Animal; Osteomielite.	
Thesaurus Nal: 	Animal genetics; Broiler chickens; Cartilage; Gallus gallus; Lameness.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (21971-1) - UPC - AP - DD -  -  -  - DIS