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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/03/2016 |
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Data da última atualização: |
22/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
COTTA, M. G.; MARRACCINI, P.; THIS, D.; LEROY, T.; BOCS, S.; DROC, G.; DUFAYARD, J. F.; DEREEPER, A.; LASHERMES, P.; ANDRADE, A. C. |
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Afiliação: |
M. G. COTTA, UFLA; P. MARRACCINI, CIRAD UMR AGAP; D. THIS, CIRAD UMR AGAP; T. LEROY, CIRAD UMR AGAP; S. BOCS, CIRAD UMR AGAP; G. DROC, CIRAD UMR AGAP; J. F. DUFAYARD, CIRAD UMR AGAP; A. DEREEPER, IRD UMR RPM; P. LASHERMES, IRD UMR RPM; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
Molecular mechanisms in the first step of ABA-mediated response to drought in Coffea canephora clones. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-50. |
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Idioma: |
Inglês |
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Conteúdo: |
Global climate changes are becoming more unpredictable and abiotic stress, such as drought and high temperatures, are key factors that affect coffee plant development and production. Although the strategies for drought adaptation in perennial plants are complex, there is genetic variability within the Coffea genus that could be used to increase tolerance. Drought tolerant clones of Coffea canephora have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, novel intracellular ABA receptors (PYL/RCARs) involved in sensing and signaling of this hormone were identified. A mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in C. canephora. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein domains identified in the predicted coffee sequences enabled to characterize these genes as family?s members of receptors (PYR/PYL/ RCAR), phosphatases (PP2Cs) or kinases (SnRK2) of ABA response pathway. Phylogenetic analysis allowed the classification of coffee-polypeptide sequences between subclasses and subfamilies. The gene structures of these three gene families were functionally annotated in Coffee Genome Hub (http://coffee-genome.org/). Tissue (leaf, seed, root and floral organ) differential expression was verified through in silico analyses. Regarding drought conditions, data from root transcriptome showed contrasting gene expression for those genes between the tolerant (Cc14, Cc73 and Cc120) and susceptible (Cc22) clones of C. canephora. By comparing gene expression profiles of only drought tolerant (Cc14, Cc73 and Cc120) clones, differential responses were observed suggesting the existence of multiple biological mechanisms for drought tolerance in coffee. All those evidences will help to identify the genetic determinism of drought tolerance essential to obtain molecular markers that could be used in coffee breeding programs. MenosGlobal climate changes are becoming more unpredictable and abiotic stress, such as drought and high temperatures, are key factors that affect coffee plant development and production. Although the strategies for drought adaptation in perennial plants are complex, there is genetic variability within the Coffea genus that could be used to increase tolerance. Drought tolerant clones of Coffea canephora have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, novel intracellular ABA receptors (PYL/RCARs) involved in sensing and signaling of this hormone were identified. A mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in C. canephora. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein domains identified in the predicted coffee sequences enabled to characterize these genes as family?s members of receptors (PYR/PYL/ RCAR), phosphatases (PP2Cs) or kinases (SnRK2) of ABA response pathway. Phylogenetic analysis allowed the classification of coffee-polypeptide sequences between subc... Mostrar Tudo |
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Palavras-Chave: |
Coffee. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03092nam a2200229 a 4500
001 2041744
005 2023-03-22
008 2015 bl uuuu u00u1 u #d
100 1 $aCOTTA, M. G.
245 $aMolecular mechanisms in the first step of ABA-mediated response to drought in Coffea canephora clones.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-50.$c2015
520 $aGlobal climate changes are becoming more unpredictable and abiotic stress, such as drought and high temperatures, are key factors that affect coffee plant development and production. Although the strategies for drought adaptation in perennial plants are complex, there is genetic variability within the Coffea genus that could be used to increase tolerance. Drought tolerant clones of Coffea canephora have been characterized as vigorous plants with high productivity throughout years under water deficit. Recently, novel intracellular ABA receptors (PYL/RCARs) involved in sensing and signaling of this hormone were identified. A mechanism of ABA transduction was proposed, involving PYR/PYL/RCARs receptors interacting with PP2Cs phosphatases and SnRK2 protein kinases. The goal of this study was to identify and characterize orthologous genes of this tripartite system in C. canephora. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, and tomato were chosen as query to search orthologous genes in sequence databases. This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCAR, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein domains identified in the predicted coffee sequences enabled to characterize these genes as family?s members of receptors (PYR/PYL/ RCAR), phosphatases (PP2Cs) or kinases (SnRK2) of ABA response pathway. Phylogenetic analysis allowed the classification of coffee-polypeptide sequences between subclasses and subfamilies. The gene structures of these three gene families were functionally annotated in Coffee Genome Hub (http://coffee-genome.org/). Tissue (leaf, seed, root and floral organ) differential expression was verified through in silico analyses. Regarding drought conditions, data from root transcriptome showed contrasting gene expression for those genes between the tolerant (Cc14, Cc73 and Cc120) and susceptible (Cc22) clones of C. canephora. By comparing gene expression profiles of only drought tolerant (Cc14, Cc73 and Cc120) clones, differential responses were observed suggesting the existence of multiple biological mechanisms for drought tolerance in coffee. All those evidences will help to identify the genetic determinism of drought tolerance essential to obtain molecular markers that could be used in coffee breeding programs.
653 $aCoffee
700 1 $aMARRACCINI, P.
700 1 $aTHIS, D.
700 1 $aLEROY, T.
700 1 $aBOCS, S.
700 1 $aDROC, G.
700 1 $aDUFAYARD, J. F.
700 1 $aDEREEPER, A.
700 1 $aLASHERMES, P.
700 1 $aANDRADE, A. C.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/04/2010 |
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Data da última atualização: |
06/05/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
COELHO, L. M.; NIELEN, S.; CARNEIRO, V. T. de C.; ARAUJO, A. C. G. de. |
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Afiliação: |
L. M. COELHO, UNB; STEPHAN NIELEN; VERA TAVARES DE CAMPOS CARNEIRO, CENARGEN; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN. |
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Título: |
Obtenção de cromossomos metafásicos de Brachiaria decumbens e Brachiaria humidicola para FISH. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 015. |
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Páginas: |
p. 49 |
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Idioma: |
Português |
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Palavras-Chave: |
Biologia celular. |
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Thesagro: |
Apomixia; Brachiaria Decumbens; Brachiaria Humidicola. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00761nam a2200193 a 4500
001 1697432
005 2024-05-06
008 2009 bl uuuu u01u1 u #d
100 1 $aCOELHO, L. M.
245 $aObtenção de cromossomos metafásicos de Brachiaria decumbens e Brachiaria humidicola para FISH.
260 $aIn: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 015.$c2009
300 $ap. 49
650 $aApomixia
650 $aBrachiaria Decumbens
650 $aBrachiaria Humidicola
653 $aBiologia celular
700 1 $aNIELEN, S.
700 1 $aCARNEIRO, V. T. de C.
700 1 $aARAUJO, A. C. G. de
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