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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
30/03/2016 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, L. F. dos; FREGAPANI, R. M.; FALCAO, L. L.; TOGAWA, R. C.; COSTA, M. M. do C.; LOPES, U. V.; GRAMACHO, K. P.; ALVES, R. M.; MICHELI, F.; MARCELLINO, L. H. |
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Afiliação: |
Lucas Ferraz dos Santos, UESC/CENARGEN; Roberta Moreira Fregapani, CENARGEN; LOENI LUDKE FALCAO, CENARGEN; ROBERTO COITI TOGAWA, CENARGEN; MARCOS MOTA DO CARMO COSTA, CENARGEN; Uilson Vanderlei Lopes, CEPLAC/CEPEC; Karina Peres Gramacho, CEPLAC/CEPEC; RAFAEL MOYSES ALVES, CPATU; Fabienne Micheli, UESC/CIRAD; LUCILIA HELENA MARCELLINO, CENARGEN. |
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Título: |
First microsatellite markers developed from cupuassu ESTs: application in diversity analysis and cross-species transferability to cacao. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
PLoS ONE, v. 11, n. 3, e0151074, 2016. |
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DOI: |
10.1371/journal.pone.0151074 |
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Idioma: |
Inglês |
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Conteúdo: |
The cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively. MenosThe cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cup... Mostrar Tudo |
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Palavras-Chave: |
Melhoramento genético. |
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Thesagro: |
Cacau; Cupuaçu; Fruta Tropical. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141829/1/2016Santos.pdf
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Marc: |
LEADER 02580naa a2200289 a 4500
001 2052633
005 2023-03-24
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0151074$2DOI
100 1 $aSANTOS, L. F. dos
245 $aFirst microsatellite markers developed from cupuassu ESTs$bapplication in diversity analysis and cross-species transferability to cacao.$h[electronic resource]
260 $c2016
520 $aThe cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively.
650 $aCacau
650 $aCupuaçu
650 $aFruta Tropical
653 $aMelhoramento genético
700 1 $aFREGAPANI, R. M.
700 1 $aFALCAO, L. L.
700 1 $aTOGAWA, R. C.
700 1 $aCOSTA, M. M. do C.
700 1 $aLOPES, U. V.
700 1 $aGRAMACHO, K. P.
700 1 $aALVES, R. M.
700 1 $aMICHELI, F.
700 1 $aMARCELLINO, L. H.
773 $tPLoS ONE$gv. 11, n. 3, e0151074, 2016.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca |
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| Registros recuperados : 51 | |
| 5. |  | GRYNBERG, P.; GUIMARÃES, L. A.; COSTA, M. M. do C.; TOGAWA, R. C.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Comprehensive profiling and characterization of Arachis stenosperma (peanut) and Meloidogyne arenaria (plant-root nematode) small-RNAs identified during the course of the infection. In: INTERNATIONAL CONFERENCE OF THE AB3C, 13., 2017, São Pedro. Proceedings... [S.l.]: AB3C, 2017. p. 170| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | GOMES, T. G.; ALVES, G. S. C.; HADI, S. I. I. A.; COSTA, M. M. do C.; MENDONCA, S.; MILLER, R. N. G.; SIQUEIRA, F. G. de. Expressão diferencial de genes em Pleurotus pulmonarius associados a degradação enzimática e destoxificação da torta de pinhão-manso. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 18.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | SALGADO, F. F.; LEAO, A. P.; GRYNBERG, P.; COSTA, M. M. do C.; TOGAWA, R. C.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Prediction and annotation of miRNAs and the preponderant role of transcription factors in the response of Elaeis guineensis Jacq to abiotic stresses of drought and salinity. In: BRAZILIAN SYMPOSIUM ON BIOINFOMATICS (BSB 2023), 16., 2023, Curitiba, PR. [Proceedings...]. Porto Alegre, RS: Sociedade Brasileira de Computação, 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | ANDRADE, E. L. P. de; LOPES JUNIOR, S.; COSTA, M. M. do C.; RAMOS, R. G. C.; FARIAS, J. L. S.; SILVA, A. M. D.; PROCOPIO, C. D. Utilização de tecnologia orientada a objetos no desenvolvimento de sistemas de informação corporativos na Embrapa. In: TURAZI, A. (Ed.). O recurso tecnologia da informação em uma instituição de pesquisa agropecuária: de 1974 a 2000. Brasília, DF: Embrapa Comunicação para Transferência de Tecnologia, 2001. p. 195-224.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 12. | | BERGMANN, J. C.; COSTA, M. M. do C.; JUNIOR, P. G. J.; BARRETO, C. C.; KRUGER, R. H.; QUIRINO, B. F. Soil bacterial community structure associated with oil palm (Elaeis guineensis) fatal yellowing disease. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 13. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H. Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 15. | | MIDORIKAWA, G. E. O.; CORREA, C. L.; NORONHA, E. F.; FERREIRA FILHO, E. X.; TOGAWA, R. C.; COSTA, M. M. do C.; SILVA JUNIOR, O. B. da; GRYNBERG, P.; MILLER, R. N. G. Analysis of the transcriptome in Aspergillus tamarii during enzymatic degradation of sugarcane bagasse. Frontiers in Bioengineering and Biotechnology, v 6, article 123, 2018. Na publicação: Orzenil Bonfim Silva-Junior.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | SARTOR, T.; FALAVIGNA, V. da S.; CATTANI, A. M.; SILVEIRA, C. P.; MALABARBA, J.; PORTO, D. D.; GRYNBERG, P.; TOGAWA, R. C.; COSTA, M. M. do C.; SANTOS, H. P. dos; PASQUALI, G.; REVERS, L. F. Caracterização da função do gene FLC-LIKE durante a transição da ENDO- para dormência na macieira. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais...FLorianópolis: SBG, 30 de maio a 2 de junho de 2023. p. 67| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 17. | | SARTOR, T.; FALAVIGNA, V. da S.; CATTANI, A. M.; SILVEIRA, C. P.; MALABARBA, J.; PORTO, D. D.; GRYNBERG, P.; TOGAWA, R. C.; COSTA, M. M. do C.; SANTOS, H. P. dos; PASQUALI, G.; REVERS, L. F. Caracterização da função do gene FLC-LIKE durante a transição da Endo- para ecodormencia na macieira. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais...Florianópolis: SBG, 2023. p. 67.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 18. | | MILLER, R. N. G.; PASSOS, M. A. N.; MENEZES, N. N. P.; SOUZA JUNIOR, M. T.; COSTA, M. M. do C.; AZEVEDO, V. C. R.; AMORIM, E. P.; PAPPAS JUNIOR, G. J.; CIAMPI, A. Y. Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4. BMC Research Notes, v.3, n.148, 2010. Disponível em:.Acesso em: 16 ago. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | MILLER, R. N. G.; PASSOS, M. A. N.; MENEZES, N. N. P.; SOUZA JUNIOR, M. T.; COSTA, M. M. do C.; AZEVEDO, V. C. R.; AMORIM, E. P.; PAPPAS JUNIOR, G. J.; CIAMPI, A. Y. Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4. BMC Research Notes, v. 3, p. 148-153, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 20. | | ARAÚJO, J. F.; CASTRO, A. R. de; COSTA, M. M. do C.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; QUIRINO, B. F.; BUSTAMANTE, M. M. C.; WILLIAMSON, L.; HANDELSMAN, J.; KRUGER, R. H. Characterization of soil bacterial assemblies in Brazilian Savanna-Like vegetation reveals acidobacteria dominance. Microbial Ecology, v. 64, p. 760-770, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 51 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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