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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
03/05/2013 |
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Data da última atualização: |
17/01/2024 |
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Autoria: |
FROTA, I. M. A.; LEITÃO, C. C. F.; COSTA, J. J. N.; HURK, R. van den; BRITO, I. R.; SARAIVA, M. V. A.; FIGUEIREDO, J. R.; SILVA, J. R. V. |
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Título: |
Effects of BMP-7 and FSH on the development of goat preantral follicles and levels of mRNA for FSH-R, BMP-7 and BMP receptors after in-vitro culture. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Animal Reproduction, v. 8, n. 1/2, p. 25-31, Jan./Jun. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to evaluate the effects of FSH and BMP-7 on growth and on expression of FSH-R, BMP-7 and BMP receptors in cultured secondary follicles. Goat secondary follicles (~200 µm) were isolated and cultured in vitro, with 5% CO2 in air at 39°C, for 6 days in the presence of BMP-7 (50 ng/ml) supplemented or not with FSH (50 ng/ml). Follicular diameter and the formation of the antrum were evaluated before and after culture. For each treatment, at the end of culture period, groups of 6 follicles were collected and, after extraction of total RNA and cDNA synthesis, the levels of mRNA for FSH-R, BMP-7 and BMP receptors in cultured secondary follicles were quantified by real time PCR. The results showed that addition of BMP-7 or FSH to culture medium stimulated growth of secondary follicles, while addition of both BMP-7 and FSH was needed to significantly increase the percentage of follicles forming an antrum and the follicular levels of mRNA for both BMP-7 and FSH-R. For BMP receptors, FSH reduced the levels of mRNA for BMPR-IA and BMP-RII in comparison with those follicles cultured in MEM alone and supplemented with BMP-7, respectively. In conclusion, like FSH, BMP-7 affects in vitro growth of cultured secondary follicles, but it stimulates antrum formation and expression of the mRNA?s for BMP-7 and FSH-R only in presence of both BMP-7 and FSH. However, the levels of mRNA for BMP-RIA and BMP-RII are reduced in follicles cultured in medium supplemented with FSH. |
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Palavras-Chave: |
Folículo ovariano; Folículos pre-antrais; Folítulo pré-antral; FSH. |
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Thesagro: |
Biotecnologia; Cabra; Caprino; Fertilidade; Genética animal; Hormônio; Ovário; Reprodução animal. |
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Categoria do assunto: |
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Marc: |
LEADER 02542naa a2200349 a 4500
001 1957252
005 2024-01-17
008 2011 bl uuuu u00u1 u #d
100 1 $aFROTA, I. M. A.
245 $aEffects of BMP-7 and FSH on the development of goat preantral follicles and levels of mRNA for FSH-R, BMP-7 and BMP receptors after in-vitro culture.$h[electronic resource]
260 $c2011
520 $aAbstract: This study aimed to evaluate the effects of FSH and BMP-7 on growth and on expression of FSH-R, BMP-7 and BMP receptors in cultured secondary follicles. Goat secondary follicles (~200 µm) were isolated and cultured in vitro, with 5% CO2 in air at 39°C, for 6 days in the presence of BMP-7 (50 ng/ml) supplemented or not with FSH (50 ng/ml). Follicular diameter and the formation of the antrum were evaluated before and after culture. For each treatment, at the end of culture period, groups of 6 follicles were collected and, after extraction of total RNA and cDNA synthesis, the levels of mRNA for FSH-R, BMP-7 and BMP receptors in cultured secondary follicles were quantified by real time PCR. The results showed that addition of BMP-7 or FSH to culture medium stimulated growth of secondary follicles, while addition of both BMP-7 and FSH was needed to significantly increase the percentage of follicles forming an antrum and the follicular levels of mRNA for both BMP-7 and FSH-R. For BMP receptors, FSH reduced the levels of mRNA for BMPR-IA and BMP-RII in comparison with those follicles cultured in MEM alone and supplemented with BMP-7, respectively. In conclusion, like FSH, BMP-7 affects in vitro growth of cultured secondary follicles, but it stimulates antrum formation and expression of the mRNA?s for BMP-7 and FSH-R only in presence of both BMP-7 and FSH. However, the levels of mRNA for BMP-RIA and BMP-RII are reduced in follicles cultured in medium supplemented with FSH.
650 $aBiotecnologia
650 $aCabra
650 $aCaprino
650 $aFertilidade
650 $aGenética animal
650 $aHormônio
650 $aOvário
650 $aReprodução animal
653 $aFolículo ovariano
653 $aFolículos pre-antrais
653 $aFolítulo pré-antral
653 $aFSH
700 1 $aLEITÃO, C. C. F.
700 1 $aCOSTA, J. J. N.
700 1 $aHURK, R. van den
700 1 $aBRITO, I. R.
700 1 $aSARAIVA, M. V. A.
700 1 $aFIGUEIREDO, J. R.
700 1 $aSILVA, J. R. V.
773 $tAnimal Reproduction$gv. 8, n. 1/2, p. 25-31, Jan./Jun. 2011.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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