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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/09/2023 |
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Data da última atualização: |
11/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AYMÉE, L.; DI AZEVEDO, M. I. N.; REIS, L.; MENDES, J.; CASTRO, F. de D. A. de; CARVALHO-COSTA, F. A. C.; SOUZA, G. N. de; LILENBAUM, W. |
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Afiliação: |
LUIZA AYMÉE, UNIVERSIDADE FEDERAL FLUMINENSE; MARIA ISABEL NOGUEIRA DI AZEVEDO, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZA REIS, UNIVERSIDADE FEDERAL FLUMINENSE; JULIA MENDES, UNIVERSIDADE FEDERAL FLUMINENSE; FÚLVIA DE FÁTIMA ALMEIDA DE CASTRO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; FILIPE ANIBAL CARVALHO-COSTA, INSTITUTO OSWALDO CRUZ; GUILHERME NUNES DE SOUZA, CNPGL; WALTER LILENBAUM, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Unconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Animals, v. 13, 2832, 2023. |
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DOI: |
https://doi.org/10.3390/ani13182832 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses. MenosBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular ana... Mostrar Tudo |
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Palavras-Chave: |
Abomasal fluid; Leptospiral infection; LipL32; Molecular analysis. |
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Thesagro: |
Aborto; Bovino; Gado; Leptospirose. |
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Thesaurus Nal: |
Abortion (animals); Cattle; Necropsy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156556/1/Unconventional-sites-for-diagnosis-of-Leptospirosis-in-bovine-anicteric-fetuses.pdf
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Marc: |
LEADER 03302naa a2200349 a 4500
001 2156556
005 2023-09-11
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ani13182832$2DOI
100 1 $aAYMÉE, L.
245 $aUnconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses.$h[electronic resource]
260 $c2023
520 $aBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses.
650 $aAbortion (animals)
650 $aCattle
650 $aNecropsy
650 $aAborto
650 $aBovino
650 $aGado
650 $aLeptospirose
653 $aAbomasal fluid
653 $aLeptospiral infection
653 $aLipL32
653 $aMolecular analysis
700 1 $aDI AZEVEDO, M. I. N.
700 1 $aREIS, L.
700 1 $aMENDES, J.
700 1 $aCASTRO, F. de D. A. de
700 1 $aCARVALHO-COSTA, F. A. C.
700 1 $aSOUZA, G. N. de
700 1 $aLILENBAUM, W.
773 $tAnimals$gv. 13, 2832, 2023.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Unidades Centrais. |
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Data corrente: |
17/10/2023 |
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Data da última atualização: |
17/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARTINAZZO, R.; MULLER, C. A.; TEIXEIRA, L. C.; STUMPF, L.; ANTUNES, w. r.; METZ, L. E. G.; VALGAS, R. A.; BAMBERG, A. L.; SILVEIRA, C. A. P. |
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Afiliação: |
ROSANE MARTINAZZO, CPACT; CAMILA ARIANA MULLER, INSTITUTO SENAI DE TECNOLOGIA EM COURO E MEIO AMBIENTE; LUCIANA COSTA TEIXEIRA, INSTITUTO SENAI DE TECNOLOGIA EM COURO E MEIO AMBIENTE; LIZETE STUMPF, UNIVERSIDADE FEDERAL DE PELOTAS; WILLIAM RODRIGUES ANTUNES, UNIVERSIDADE FEDERAL DE PELOTAS; LISIANE EMILIA GRAMS METZ, UNIVERSIDADE FEDERAL DE PELOTAS; RICARDO ALEXANDRE VALGAS, GEM; ADILSON LUIS BAMBERG, CPACT; CARLOS AUGUSTO POSSER SILVEIRA, CPACT. |
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Título: |
Leather-based fertilizers from Personal Protective Equipment (PPE) reverse logistics: Technical efficiency and environmental safety. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Resources, Conservation & Recycling Advances, v. 18, 200153, Oct. 2023. |
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ISSN: |
2667-3789 |
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DOI: |
https://doi.org/10.1016/j.rcradv.2023.200153 |
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Idioma: |
Inglês |
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Conteúdo: |
Agriculture and industry are seeking healthy food and enough goods to meet the global demand without causing environmental impacts, which motivates the search for sustainable and eco-efficient technologies. This study focuses on reverse logistics in the Personal Protective Equipment (PPE) industry. Chrome-tanned leather shavings from the manufacture of PPE and post-use leather PPE were used to produce leather-based fertilizers (LBFs), which were evaluated for their agronomic efficiency and food safety. N-rich liquid LBFs were successfully produced through hydrolysis, either with or without previous chromium extraction. At the dose of maximum technical efficiency, LBFs are safe, with acceptable levels of Cr in the plant biomass and the soil compartments. However, LBF overdoses promote Cr accumulation in plants and soils, which demonstrates the need for a rigorous control over the fertilization management to ensure successful PPE reverse logistics. |
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Palavras-Chave: |
Economia circular; Eficiência agronômica; Remoção de cromo; Resíduos de couro curtido ao cromo. |
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Thesagro: |
Couro; Cromo. |
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Thesaurus NAL: |
Chromium; Leather; Processing residues. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1157292/1/ArtigoMartinazzoetal2023.pdf
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Marc: |
LEADER 02040naa a2200349 a 4500
001 2157292
005 2023-10-17
008 2023 bl uuuu u00u1 u #d
022 $a2667-3789
024 7 $ahttps://doi.org/10.1016/j.rcradv.2023.200153$2DOI
100 1 $aMARTINAZZO, R.
245 $aLeather-based fertilizers from Personal Protective Equipment (PPE) reverse logistics$bTechnical efficiency and environmental safety.$h[electronic resource]
260 $c2023
520 $aAgriculture and industry are seeking healthy food and enough goods to meet the global demand without causing environmental impacts, which motivates the search for sustainable and eco-efficient technologies. This study focuses on reverse logistics in the Personal Protective Equipment (PPE) industry. Chrome-tanned leather shavings from the manufacture of PPE and post-use leather PPE were used to produce leather-based fertilizers (LBFs), which were evaluated for their agronomic efficiency and food safety. N-rich liquid LBFs were successfully produced through hydrolysis, either with or without previous chromium extraction. At the dose of maximum technical efficiency, LBFs are safe, with acceptable levels of Cr in the plant biomass and the soil compartments. However, LBF overdoses promote Cr accumulation in plants and soils, which demonstrates the need for a rigorous control over the fertilization management to ensure successful PPE reverse logistics.
650 $aChromium
650 $aLeather
650 $aProcessing residues
650 $aCouro
650 $aCromo
653 $aEconomia circular
653 $aEficiência agronômica
653 $aRemoção de cromo
653 $aResíduos de couro curtido ao cromo
700 1 $aMULLER, C. A.
700 1 $aTEIXEIRA, L. C.
700 1 $aSTUMPF, L.
700 1 $aANTUNES, w. r.
700 1 $aMETZ, L. E. G.
700 1 $aVALGAS, R. A.
700 1 $aBAMBERG, A. L.
700 1 $aSILVEIRA, C. A. P.
773 $tResources, Conservation & Recycling Advances$gv. 18, 200153, Oct. 2023.
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