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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/05/2014 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARGÔLO FILHO, R. C.; COSTA, R. L.; PINHEIRO, H. E.; CORRÊA, F. M.; VALICENTE, F. H.; POMELLA, A. W. V.; LOGUERCIO, L. L. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Toxins, v. 6, p. 1598-1614, 2014. |
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DOI: |
10.3390/toxins6051598 |
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Idioma: |
Inglês |
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Conteúdo: |
Bioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems. MenosBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activ... Mostrar Tudo |
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Thesagro: |
Toxina. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02484naa a2200217 a 4500
001 1987282
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
024 7 $a10.3390/toxins6051598$2DOI
100 1 $aARGÔLO FILHO, R. C.
245 $aRequirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development.$h[electronic resource]
260 $c2014
520 $aBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems.
650 $aToxina
700 1 $aCOSTA, R. L.
700 1 $aPINHEIRO, H. E.
700 1 $aCORRÊA, F. M.
700 1 $aVALICENTE, F. H.
700 1 $aPOMELLA, A. W. V.
700 1 $aLOGUERCIO, L. L.
773 $tToxins$gv. 6, p. 1598-1614, 2014.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/08/2021 |
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Data da última atualização: |
05/08/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
COÊLHO, L. M.; SANTOS, A. R. de S.; TORGA, P. P.; FERREIRA, M. E.; VIANELLO, R. P.; PEREIRA, H. S.; MELO, L. C.; SOUZA, T. L. P. O. de. |
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Afiliação: |
LAYSLA MORAIS COÊLHO, doutoranda UFG; ANA RÚBIA DE SÁ SANTOS, estagiária CNPAF; PAULA PEREIRA TORGA, CNPAF; MARCIO ELIAS FERREIRA, Cenargen; ROSANA PEREIRA VIANELLO, CNPAF; HELTON SANTOS PEREIRA, CNPAF; LEONARDO CUNHA MELO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF. |
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Título: |
Teste de progênies para resistência ao crestamento-bacteriano-aureolado na população BRS Estilo x ZAA-12 (Gene Pse-2) utilizando marcador SCAR. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: SEMINÁRIO JOVENS TALENTOS, 14., 2020, Santo Antônio de Goiás. Resumos... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2021. |
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Páginas: |
p. 77. |
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ISBN: |
978-65-87380-12-4 |
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Idioma: |
Português |
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Conteúdo: |
A bactéria Pseudomonas syringae pv. phaseolicola (Psp) é um organismo quarentenário de alto risco para a produção de feijão (Phaseolus vulgaris L.) no Brasil, agente causal do crestamento-bacteriano-aureolado. O objetivo deste trabalho foi realizar um teste de progênies para a seleção de plantas homozigotas para o alelo Pse-2, que confere resistência à doença, com o auxílio de marcador SCAR (Sequence Characterized Amplified Regions). |
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Thesagro: |
Crestamento; Feijão; Marcador Molecular; Phaseolus Vulgaris; Variedade Resistente. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 01401nam a2200277 a 4500
001 2133243
005 2021-08-05
008 2021 bl uuuu u01u1 u #d
020 $a978-65-87380-12-4
100 1 $aCOÊLHO, L. M.
245 $aTeste de progênies para resistência ao crestamento-bacteriano-aureolado na população BRS Estilo x ZAA-12 (Gene Pse-2) utilizando marcador SCAR.$h[electronic resource]
260 $aIn: SEMINÁRIO JOVENS TALENTOS, 14., 2020, Santo Antônio de Goiás. Resumos... Santo Antônio de Goiás: Embrapa Arroz e Feijão$c2021
300 $ap. 77.
520 $aA bactéria Pseudomonas syringae pv. phaseolicola (Psp) é um organismo quarentenário de alto risco para a produção de feijão (Phaseolus vulgaris L.) no Brasil, agente causal do crestamento-bacteriano-aureolado. O objetivo deste trabalho foi realizar um teste de progênies para a seleção de plantas homozigotas para o alelo Pse-2, que confere resistência à doença, com o auxílio de marcador SCAR (Sequence Characterized Amplified Regions).
650 $aCrestamento
650 $aFeijão
650 $aMarcador Molecular
650 $aPhaseolus Vulgaris
650 $aVariedade Resistente
700 1 $aSANTOS, A. R. de S.
700 1 $aTORGA, P. P.
700 1 $aFERREIRA, M. E.
700 1 $aVIANELLO, R. P.
700 1 $aPEREIRA, H. S.
700 1 $aMELO, L. C.
700 1 $aSOUZA, T. L. P. O. de
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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