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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pesca e Aquicultura. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pesca e Aquicultura; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
03/01/2018 |
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Data da última atualização: |
08/02/2019 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
CARRIERO, M. M.; HENRIQUE-SILVA, F.; CAETANO, A. R.; LOBO, F. P.; ALVES, A. L.; VARELA, E. S.; COLLADO, M. del; MOREIRA, G. S. A.; MAIA, A. A. M. |
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Afiliação: |
MATEUS M. CARRIERO, USP, UFSCar; FLÁVIO HENRIQUE-SILVA, UFSCar; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO, CNPTIA; ANDERSON LUIS ALVES, CNPASA; EDUARDO SOUSA VARELA, CNPASA; MAITE DEL COLLADO, USP; GABRIEL S. A. MOREIRA, USP; ANTONIO A. M. MAIA, USP. |
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Título: |
Characterization and gene expression analysis of pacu (Piaractus mesopotamicus) inducible nitric oxide synthase (iNOS) following Aeromonas dhakensis infection. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Fish and Shellfish Immunology, v. 74, p. 94-100, 2018. |
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DOI: |
10.1016/j.fsi.2017.12.025 |
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Idioma: |
Inglês |
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Notas: |
Short communication. |
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Conteúdo: |
Nitric oxide (NO) is an important effector molecule which is involved in a myriad of biological processes, including immune responses against pathogens such as parasites, virus and bacteria. During the inflammatory processes in vertebrates, NO is produced by the inducible nitric oxide synthase (iNOS) enzyme in practically all nucleated cells to suppress or kill intracellular pathogens. The aim of the present study was to characterize the full coding region of the iNOS gene of pacu (Piaractus mesopotamicus), an economically and ecologically important South American fish species, and to analyze mRNA expression levels following intraperitoneal infection with the pathogenic bacterium Aeromonas dhakensis by means of quantitative real time PCR (qPCR). The results showed that the pacu iNOS transcript is 3237 bp in length, encoding a putative protein composed of 1078 amino acid residues. The amino acid sequence showed similarities ranging from 69.03% to 94.34% with other teleost fish and 57.70% with the human iNOS, with all characteristic domains and cofactor binding sites of the enzyme detected. Phylogenetic analysis showed that the iNOS from the red-bellied piranha, another South American characiform, was the closest related sequence to the pacu iNOS. iNOS transcripts were constitutively detected in the liver, spleen and head kidney, and there was a significant upregulation in the liver and spleen at 12, 24 and 48 h after infection with A. dhakensis. No significant variations were observed in the head kidney during the periods analyzed. These results show that iNOS expression was induced by A. dhakensis infection and suggest that this enzyme may be involved in the response to this bacterium in pacu. MenosNitric oxide (NO) is an important effector molecule which is involved in a myriad of biological processes, including immune responses against pathogens such as parasites, virus and bacteria. During the inflammatory processes in vertebrates, NO is produced by the inducible nitric oxide synthase (iNOS) enzyme in practically all nucleated cells to suppress or kill intracellular pathogens. The aim of the present study was to characterize the full coding region of the iNOS gene of pacu (Piaractus mesopotamicus), an economically and ecologically important South American fish species, and to analyze mRNA expression levels following intraperitoneal infection with the pathogenic bacterium Aeromonas dhakensis by means of quantitative real time PCR (qPCR). The results showed that the pacu iNOS transcript is 3237 bp in length, encoding a putative protein composed of 1078 amino acid residues. The amino acid sequence showed similarities ranging from 69.03% to 94.34% with other teleost fish and 57.70% with the human iNOS, with all characteristic domains and cofactor binding sites of the enzyme detected. Phylogenetic analysis showed that the iNOS from the red-bellied piranha, another South American characiform, was the closest related sequence to the pacu iNOS. iNOS transcripts were constitutively detected in the liver, spleen and head kidney, and there was a significant upregulation in the liver and spleen at 12, 24 and 48 h after infection with A. dhakensis. No significant variation... Mostrar Tudo |
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Palavras-Chave: |
Aeromonas dhakensis; INOS; Óxido nítrico; Resposta imunológica. |
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Thesagro: |
Pacu; Peixe de água doce. |
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Thesaurus Nal: |
Aeromonas; Fish diseases; Immune response; Inducible nitric oxide synthase; Nitric oxide; Piaractus mesopotamicus. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02913naa a2200385 a 4500
001 2084169
005 2019-02-08
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.fsi.2017.12.025$2DOI
100 1 $aCARRIERO, M. M.
245 $aCharacterization and gene expression analysis of pacu (Piaractus mesopotamicus) inducible nitric oxide synthase (iNOS) following Aeromonas dhakensis infection.$h[electronic resource]
260 $c2018
500 $aShort communication.
520 $aNitric oxide (NO) is an important effector molecule which is involved in a myriad of biological processes, including immune responses against pathogens such as parasites, virus and bacteria. During the inflammatory processes in vertebrates, NO is produced by the inducible nitric oxide synthase (iNOS) enzyme in practically all nucleated cells to suppress or kill intracellular pathogens. The aim of the present study was to characterize the full coding region of the iNOS gene of pacu (Piaractus mesopotamicus), an economically and ecologically important South American fish species, and to analyze mRNA expression levels following intraperitoneal infection with the pathogenic bacterium Aeromonas dhakensis by means of quantitative real time PCR (qPCR). The results showed that the pacu iNOS transcript is 3237 bp in length, encoding a putative protein composed of 1078 amino acid residues. The amino acid sequence showed similarities ranging from 69.03% to 94.34% with other teleost fish and 57.70% with the human iNOS, with all characteristic domains and cofactor binding sites of the enzyme detected. Phylogenetic analysis showed that the iNOS from the red-bellied piranha, another South American characiform, was the closest related sequence to the pacu iNOS. iNOS transcripts were constitutively detected in the liver, spleen and head kidney, and there was a significant upregulation in the liver and spleen at 12, 24 and 48 h after infection with A. dhakensis. No significant variations were observed in the head kidney during the periods analyzed. These results show that iNOS expression was induced by A. dhakensis infection and suggest that this enzyme may be involved in the response to this bacterium in pacu.
650 $aAeromonas
650 $aFish diseases
650 $aImmune response
650 $aInducible nitric oxide synthase
650 $aNitric oxide
650 $aPiaractus mesopotamicus
650 $aPacu
650 $aPeixe de água doce
653 $aAeromonas dhakensis
653 $aINOS
653 $aÓxido nítrico
653 $aResposta imunológica
700 1 $aHENRIQUE-SILVA, F.
700 1 $aCAETANO, A. R.
700 1 $aLOBO, F. P.
700 1 $aALVES, A. L.
700 1 $aVARELA, E. S.
700 1 $aCOLLADO, M. del
700 1 $aMOREIRA, G. S. A.
700 1 $aMAIA, A. A. M.
773 $tFish and Shellfish Immunology$gv. 74, p. 94-100, 2018.
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Embrapa Agricultura Digital (CNPTIA) |
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| 2. |  | SOUZA, M. E. P. de; CARVALHO, A. M. X. de; DELIBERALI, D. de C.; JUCKSCH, I.; BROWN, G. G.; MENDONÇA, E. S.; CARDOSO, I. M. Vermicomposting with rock powder increases plant growth. Applied Soil Ecology, v. 69, (nesp), p. 56-60, July 2013. Special issue: Progress and Priorities in Latin American Oligochaete Research.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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