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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
23/03/2017 |
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Data da última atualização: |
28/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MILLER, A. M.; FIGUEIREDO, J. E. F.; CHAVES, C. L.; RUAS, E. A.; BALBI-PEÑA, M. I.; COLAUTO, N. B.; PACCOLA-MEIRELLES, L. D. |
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Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
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Título: |
Genomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, 2016. |
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DOI: |
10.4238/gmr.15049452 |
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Idioma: |
Inglês |
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Conteúdo: |
Measures to control maize white spot (MWS) caused by Pantoea ananatis are preferentially based on resistant cultivars. A lack of knowledge on the genetic variability of pathogens could interfere with the development and utilization of controlling strategies in this pathosystem. The main goals of this study were to investigate the genetic variability of 90 P. ananatis isolates from three different eco-geographical regions of Brazil by amplified fragment length polymorphism (AFLP), and to determine the presence of a universal P. ananatis plasmid in isolates from tropical Brazil. Analysis of genetic similarity by AFLP allowed us to categorize the 90 isolates into two groups. However, no correlation between the collecting sites and genetic groupings was observed. The polymorphism percentage found in P. ananatis ranged between 24.64 and 92.46%, and genetic diversity was calculated to be 0.07-0.09. The analysis of molecular variance showed that 99.18% of genetic variability was within the populations, providing evidence that evolutionary forces were acting on these populations. All P. ananatis isolates showed the P. ananatis universal plasmid (280 or 352 kb). This is the first report on the presence of a universal P. ananatis plasmid from MWS lesions in the tropical area. |
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Palavras-Chave: |
Bactérias fitopatogênicas; Genotipagem de alelos. |
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Thesagro: |
Doença de planta; Milho; Polimorfismo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158015/1/Genomic-variability.pdf
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Marc: |
LEADER 02092naa a2200265 a 4500
001 2067524
005 2017-03-28
008 2016 bl uuuu u00u1 u #d
024 7 $a10.4238/gmr.15049452$2DOI
100 1 $aMILLER, A. M.
245 $aGenomic variability of Pantoea ananatis in maize white spot lesions assessed by AFLP markers.$h[electronic resource]
260 $c2016
520 $aMeasures to control maize white spot (MWS) caused by Pantoea ananatis are preferentially based on resistant cultivars. A lack of knowledge on the genetic variability of pathogens could interfere with the development and utilization of controlling strategies in this pathosystem. The main goals of this study were to investigate the genetic variability of 90 P. ananatis isolates from three different eco-geographical regions of Brazil by amplified fragment length polymorphism (AFLP), and to determine the presence of a universal P. ananatis plasmid in isolates from tropical Brazil. Analysis of genetic similarity by AFLP allowed us to categorize the 90 isolates into two groups. However, no correlation between the collecting sites and genetic groupings was observed. The polymorphism percentage found in P. ananatis ranged between 24.64 and 92.46%, and genetic diversity was calculated to be 0.07-0.09. The analysis of molecular variance showed that 99.18% of genetic variability was within the populations, providing evidence that evolutionary forces were acting on these populations. All P. ananatis isolates showed the P. ananatis universal plasmid (280 or 352 kb). This is the first report on the presence of a universal P. ananatis plasmid from MWS lesions in the tropical area.
650 $aDoença de planta
650 $aMilho
650 $aPolimorfismo
653 $aBactérias fitopatogênicas
653 $aGenotipagem de alelos
700 1 $aFIGUEIREDO, J. E. F.
700 1 $aCHAVES, C. L.
700 1 $aRUAS, E. A.
700 1 $aBALBI-PEÑA, M. I.
700 1 $aCOLAUTO, N. B.
700 1 $aPACCOLA-MEIRELLES, L. D.
773 $tGenetics and Molecular Research, Ribeirão Preto, 2016.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
07/12/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LISEI-DE-SÁ, M. e; RODRIGUES‑SILVA, P. L.; MORGANTE, C. V.; MELO, B. P. de; LOURENCO, I. T.; ARRAES, F. B. M.; SOUSA, J. P. A.; GALBIERI, R.; AMORIM, R. M. S.; LINS, C. B. J. de; MACEDO, L. L. P. de; MOREIRA, V. J.; FERREIRA, G. F.; RIBEIRO, T. P.; FRAGOSO, R. da R.; SILVA, M. C. M. da; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. |
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Afiliação: |
MARIA E LISEI-DE-SÁ, Empresa de Pesquisa Agropecuária de Minas Gerais; PAOLO L. RODRIGUES‑SILVA, UCB; CAROLINA VIANNA MORGANTE, CPATSA; BRUNO PAES DE MELO, INCT PlantStress Biotech; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; FABRICIO B. M. ARRAES, INCT PlantStress Biotech; JOÃO P. A. SOUSA, UCB; RAFAEL GALBIERI, Instituto Matogrossense do Algodão; REGINA M. S. AMORIM; CAMILA B. J. DE LINS; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; VALDEIR J. MOREIRA, UNB; GILANNA F. FERREIRA; THUANNE P. RIBEIRO, INCT PlantStress Biotech; RODRIGO DA ROCHA FRAGOSO, CPAC; MARIA CRISTINA MATTAR DA SILVA, Cenargen; JANICE DE ALMEIDA-ENGLER, UMR Institut Sophia Agrobiotech INRA/CNRS/UNS, France; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Planta, v. 254, 2021. |
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DOI: |
https://doi.org/10.1007/s00425-021-03776-0 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabela T. Lourenço-Tessutti; Leonardo L. P. Macedo; Rodrigo R. Fragoso; Maria C. M. Silva; Maria F. Grossi-de-Sa. |
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Conteúdo: |
Root-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to target multiple genes to promote M. incognita tolerance in cotton without phenotypic penalty in transgenic plants. MenosRoot-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to t... Mostrar Tudo |
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Palavras-Chave: |
Fitonematóide; Interfering RNA; Nematóides das galhas. |
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Thesagro: |
Algodão; Gossypium Hirsutum; Meloidogyne Incognita; Nematóide. |
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Thesaurus NAL: |
Gene silencing. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 02991naa a2200445 a 4500
001 2137487
005 2021-12-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00425-021-03776-0$2DOI
100 1 $aLISEI-DE-SÁ, M. e
245 $aPyramiding dsRNAs increases phytonematode tolerance in cotton plants.$h[electronic resource]
260 $c2021
500 $aNa publicação: Isabela T. Lourenço-Tessutti; Leonardo L. P. Macedo; Rodrigo R. Fragoso; Maria C. M. Silva; Maria F. Grossi-de-Sa.
520 $aRoot-knot nematodes (RKN) represent one of the most damaging plant-parasitic nematode genera worldwide. RNAi-mediated suppression of essential nematode genes provides a novel biotechnological strategy for the development of sustainable pest-control methods. Here, we used a Host Induced Gene Silencing (HIGS) approach by stacking dsRNA sequences into a T-DNA construct to target three essential RKN genes: cysteine protease (Mi-cpl), isocitrate lyase (Mi-icl), and splicing factor (Mi-sf), called dsMinc1, driven by the pUceS8.3 constitutive soybean promoter. Transgenic dsMinc1-T4 plants infected with Meloidogyne incognita showed a signifcant reduction in gall formation (57?64%) and egg masses production (58?67%), as well as in the estimated reproduction factor (60?78%), compared with the susceptible non-transgenic cultivar. Galls of the RNAi lines are smaller than the wild-type (WT) plants, whose root systems exhibited multiple welldeveloped root swellings. Transcript levels of the three RKN-targeted genes decreased 13- to 40-fold in nematodes from transgenic cotton galls, compared with those from control WT galls. Finally, the development of non-feeding males in transgenic plants was 2?6 times higher than in WT plants, indicating a stressful environment for nematode development after RKN gene silencing. Data strongly support that HIGS of essential RKN genes is an efective strategy to improve cotton plant tolerance. This study presents the frst application of dsRNA sequences to target multiple genes to promote M. incognita tolerance in cotton without phenotypic penalty in transgenic plants.
650 $aGene silencing
650 $aAlgodão
650 $aGossypium Hirsutum
650 $aMeloidogyne Incognita
650 $aNematóide
653 $aFitonematóide
653 $aInterfering RNA
653 $aNematóides das galhas
700 1 $aRODRIGUES‑SILVA, P. L.
700 1 $aMORGANTE, C. V.
700 1 $aMELO, B. P. de
700 1 $aLOURENCO, I. T.
700 1 $aARRAES, F. B. M.
700 1 $aSOUSA, J. P. A.
700 1 $aGALBIERI, R.
700 1 $aAMORIM, R. M. S.
700 1 $aLINS, C. B. J. de
700 1 $aMACEDO, L. L. P. de
700 1 $aMOREIRA, V. J.
700 1 $aFERREIRA, G. F.
700 1 $aRIBEIRO, T. P.
700 1 $aFRAGOSO, R. da R.
700 1 $aSILVA, M. C. M. da
700 1 $aALMEIDA-ENGLER, J. de
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 254, 2021.
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