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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2017 |
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Data da última atualização: |
02/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MACEDO, L. L. P.; SOUZA JUNIOR, J. D. D. de; COELHO, R. R.; FONSECA, F. C. A.; FIRMINO, A. A. P.; SILVA, M. C. M.; FRAGOSO, R. R.; ALBUQUERQUE, E. V. S.; SILVA, M. S.; ENGLER, J. de A.; TERRA, W. R.; GROSSI-DE-SA, M. F. |
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Afiliação: |
LEONARDO LIMA PEPINO DE MACEDO, CENARGEN; J. D. ANTONINO DE SOUZA JUNIOR, UNB; R. R. COELHO, UNB; F. C. A. FONSECA, UNB; A. A. P. FIRMINO, UFRGS; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; RODRIGO DA ROCHA FRAGOSO, CPAC; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN; MARILIA SANTOS SILVA, CENARGEN; J. DE ALMEIDA ENGLER, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE; W. R. TERRA, USP; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
Knocking down chitin synthase 2 by RNAi is lethal to the cotton boll weevil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Biotechnology Research and Innovation, v. 1, p. 72-86, 2017. |
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DOI: |
10.1016/j.biori.2017.04.001 |
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Idioma: |
Inglês |
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Conteúdo: |
The cotton boll weevil (Anthonomus grandis) is the most destructive cotton insect pest in Brazil. The endophytic habit of this insect makes difficult its chemical control. Chitin synthase (CHS) is an integral membrane glycosyltransferase that is essential for chitin chain polymerization and deposition in insect chitinous structures, such as the peritrophic membrane (PM). Because it is not present in plants or vertebrates, CHS can be considered a promising target for eco-friendly biotechnological approaches, such as RNA interference (RNAi)-mediated gene silencing. Considering the relevance of CHS genes in the chitin biosynthetic pathway in insects, we report here the molecular cloning of the full-length CHS2 cDNA from the cotton boll weevil, and its functional validation via RNAi. The AgraCHS2 cDNA sequence is 4,869 bp, with a 4,446 bp open reading frame that encodes a predicted protein with 1,482 amino acid residues. Predicted protein has high similarity (53 to 78%) with other insects CHS. Moreover, only one copy is present in A. grandis genome. Transcriptional analysis showed that AgraCHS2 transcripts are restricted to the insect midgut at the third-larval instar and adult stages, which are considered the main feeding stages. RNAi-mediated knockdown of the AgraCHS2 affected A. grandis development, resulting in oviposition reduction of 93% and leading to 100% adult mortality. These data, in addition to the observation of PM severe disorganization in the midgut after AgraCHS2 knock-down, suggest AgraCHS2 as a promising target for developing RNAi-based biotechnological alternatives to specifically control the cotton boll weevil. MenosThe cotton boll weevil (Anthonomus grandis) is the most destructive cotton insect pest in Brazil. The endophytic habit of this insect makes difficult its chemical control. Chitin synthase (CHS) is an integral membrane glycosyltransferase that is essential for chitin chain polymerization and deposition in insect chitinous structures, such as the peritrophic membrane (PM). Because it is not present in plants or vertebrates, CHS can be considered a promising target for eco-friendly biotechnological approaches, such as RNA interference (RNAi)-mediated gene silencing. Considering the relevance of CHS genes in the chitin biosynthetic pathway in insects, we report here the molecular cloning of the full-length CHS2 cDNA from the cotton boll weevil, and its functional validation via RNAi. The AgraCHS2 cDNA sequence is 4,869 bp, with a 4,446 bp open reading frame that encodes a predicted protein with 1,482 amino acid residues. Predicted protein has high similarity (53 to 78%) with other insects CHS. Moreover, only one copy is present in A. grandis genome. Transcriptional analysis showed that AgraCHS2 transcripts are restricted to the insect midgut at the third-larval instar and adult stages, which are considered the main feeding stages. RNAi-mediated knockdown of the AgraCHS2 affected A. grandis development, resulting in oviposition reduction of 93% and leading to 100% adult mortality. These data, in addition to the observation of PM severe disorganization in the midgut after AgraCHS2... Mostrar Tudo |
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Palavras-Chave: |
Insect pest control; Membrane; Peritrophic; RNAi (RNA interference). |
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Thesaurus Nal: |
Midgut. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168697/1/1-s2.0-S2452072117300096-main.pdf
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Marc: |
LEADER 02586naa a2200325 a 4500
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008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.biori.2017.04.001$2DOI
100 1 $aMACEDO, L. L. P.
245 $aKnocking down chitin synthase 2 by RNAi is lethal to the cotton boll weevil.
260 $c2017
520 $aThe cotton boll weevil (Anthonomus grandis) is the most destructive cotton insect pest in Brazil. The endophytic habit of this insect makes difficult its chemical control. Chitin synthase (CHS) is an integral membrane glycosyltransferase that is essential for chitin chain polymerization and deposition in insect chitinous structures, such as the peritrophic membrane (PM). Because it is not present in plants or vertebrates, CHS can be considered a promising target for eco-friendly biotechnological approaches, such as RNA interference (RNAi)-mediated gene silencing. Considering the relevance of CHS genes in the chitin biosynthetic pathway in insects, we report here the molecular cloning of the full-length CHS2 cDNA from the cotton boll weevil, and its functional validation via RNAi. The AgraCHS2 cDNA sequence is 4,869 bp, with a 4,446 bp open reading frame that encodes a predicted protein with 1,482 amino acid residues. Predicted protein has high similarity (53 to 78%) with other insects CHS. Moreover, only one copy is present in A. grandis genome. Transcriptional analysis showed that AgraCHS2 transcripts are restricted to the insect midgut at the third-larval instar and adult stages, which are considered the main feeding stages. RNAi-mediated knockdown of the AgraCHS2 affected A. grandis development, resulting in oviposition reduction of 93% and leading to 100% adult mortality. These data, in addition to the observation of PM severe disorganization in the midgut after AgraCHS2 knock-down, suggest AgraCHS2 as a promising target for developing RNAi-based biotechnological alternatives to specifically control the cotton boll weevil.
650 $aMidgut
653 $aInsect pest control
653 $aMembrane
653 $aPeritrophic
653 $aRNAi (RNA interference)
700 1 $aSOUZA JUNIOR, J. D. D. de
700 1 $aCOELHO, R. R.
700 1 $aFONSECA, F. C. A.
700 1 $aFIRMINO, A. A. P.
700 1 $aSILVA, M. C. M.
700 1 $aFRAGOSO, R. R.
700 1 $aALBUQUERQUE, E. V. S.
700 1 $aSILVA, M. S.
700 1 $aENGLER, J. de A.
700 1 $aTERRA, W. R.
700 1 $aGROSSI-DE-SA, M. F.
773 $tBiotechnology Research and Innovation$gv. 1, p. 72-86, 2017.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 43. |  | DLUGOSZ, F. L.; ROSOT, M. A. D.; ROSOT, N. C.; OLIVEIRA, Y. M. M.; KURASZ, G. Uso do sensoriamento remoto e SIG para o zoneamento ecológico da reserva florestal Embrapa/Epagri em Caçador - SC. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 2., 2003, Colombo. Anais. Colombo: Embrapa Florestas, 2003. 047A. 1 Cd-Rom. (Embrapa Florestas. Documentos, 86). Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 45. | | OLIVEIRA, M. F.; MATTOS, P. P. de; GARRASTAZU, M. C.; BRAZ, E. M.; FIGUEIREDO FILHO, A.; ROSOT, N. C. Análise da estrutura horizontal por densidade de Kernel como subsídio ao manejo florestal na Amazônia. Pesquisa Florestal Brasileira, Colombo, v. 41, e202002098, 2021. 14 p.| Biblioteca(s): Embrapa Florestas. |
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| 47. | | MAUSBACH, M. M.; MATTOS, P. P. de; ROSOT, N. C.; EMERENCIANO, D. B.; FERNANDES, A. dos M.; SOTO, S. J. Potencial dendrocronológico de espécies arbóreas de Moçambique. In: SIMPÓSIO BRASIL-ALEMANHA, 4., 2009, Curitiba. Desenvolvimento sustentável: resumos. Curitiba: Centro de Cooperação Internacional Brasil-Alemanha: UFPR, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 50. | | CARDOSO, D. J.; ROSOT, M. A. D.; GARRASTAZU, M. C.; ROSOT, N. C.; TONIOLO JÚNIOR, L.; OLIVEIRA, K. A. de. Recommended thinning regimes for Araucaria angustifolia plantations on small properties in southern Brazil: a case study. Advances in Forestry Science, Cuiabá, v. 4, n. 4, p. 211-218, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Florestas. |
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| 54. | | OLIVEIRA, M. F.; BRAZ, E. M.; MATTOS, P. P. de; CANETTI, A.; BASSO, R. O.; ROSOT, N. C. Padrão de crescimento e diâmetro ótimo de corte de cambará no município de Santa Carmem, microrregião de Sinop, MT. Colombo: Embrapa Florestas, 2015. 5 p. (Embrapa Florestas. Comunicado técnico, 364).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Florestas. |
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| 58. | | OLIVEIRA, M. F.; MATTOS, P. P. de; GARRASTAZU, M. C.; BRAZ, E. M.; ROSOT, N. C.; BASSO, R. O. Determinação de intensidades amostrais para a estimativa da distribuição diamétrica das espécies Qualea albiflora e Goupia glabra de Sinop - MT. In: SIMPÓSIO NACIONAL DE INVENTÁRIO FLORESTAL, 2., 2013, Curitiba. Anais. Brasília, DF: Serviço Florestal Brasileiro, 2013. p. 51. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 59. | | OLIVEIRA, M. F. de; MATTOS, P. P. de; GARRASTAZU, M. C.; BRAZ, E. M.; ROSOT, N. C. Determination of sampling intensities to estimate diameter distribution in Amazon Forest. In: CONGRESO LATINOAMERICANO DE IUFRO, 3., 2013, San José. Bosques, competitividad y territorios sostenibles. San José: CATIE; [S.l.]: IUFRO, 2013. 1 p. IUFROLAT 2013. Disponibilizado online. Resumen.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 76 | |
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