|
|
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
Data corrente: |
20/04/2016 |
Data da última atualização: |
21/07/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LACERDA, A. F.; SARTO, R. P. del; SILVA, M. S.; VASCONCELOS, E. A. R. de; COELHO, R. R.; SANTOS, V. O. dos; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. C. M. da; GROSSI-DE-SA, M. F. |
Afiliação: |
ARIANE FERREIRA LACERDA, Universidade Federal do Rio Grande do Norte; RAFAEL PERSEGHINI DEL SARTO, Centro Universitário do Distrito Federal; MARILIA SANTOS SILVA, Cenargen; ERICO AUGUSTO ROSAS DE VASCONCELOS; ROBERTA RAMOS COELHO; VANESSA OLINTO DOS SANTOS, Universidade Federal do Rio Grande do Norte; CLAUDIA VIEIRA GODOY, CNPSO; CLAUDINE DINALI SANTOS SEIXAS, CNPSO; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
3 Biotech, v. 6, n. 1, p. 59, Feb. 2016. |
ISSN: |
2190-5738 |
DOI: |
10.1007/s13205-015-0320-7 |
Idioma: |
Inglês |
Conteúdo: |
Plant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases. MenosPlant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian ru... Mostrar Tudo |
Thesagro: |
Algodão; Doença de planta; Fungo; Soja. |
Thesaurus Nal: |
Cotton; Fungi; Plant diseases and disorders; Soybeans. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142476/1/the-recombinant-pea....pdf
|
Marc: |
LEADER 02860naa a2200349 a 4500 001 2043768 005 2017-07-21 008 2016 bl uuuu u00u1 u #d 022 $a2190-5738 024 7 $a10.1007/s13205-015-0320-7$2DOI 100 1 $aLACERDA, A. F. 245 $aThe recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora.$h[electronic resource] 260 $c2016 520 $aPlant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases. 650 $aCotton 650 $aFungi 650 $aPlant diseases and disorders 650 $aSoybeans 650 $aAlgodão 650 $aDoença de planta 650 $aFungo 650 $aSoja 700 1 $aSARTO, R. P. del 700 1 $aSILVA, M. S. 700 1 $aVASCONCELOS, E. A. R. de 700 1 $aCOELHO, R. R. 700 1 $aSANTOS, V. O. dos 700 1 $aGODOY, C. V. 700 1 $aSEIXAS, C. D. S. 700 1 $aSILVA, M. C. M. da 700 1 $aGROSSI-DE-SA, M. F. 773 $t3 Biotech$gv. 6, n. 1, p. 59, Feb. 2016.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 46 | |
6. | | TARDIN, F. D.; RODRIGUES, J. A. S.; COELHO, R. R. Cultivares. In: RODRIGUES, J. A. S. (Ed.). Cultivo do sorgo. 6. ed. Sete Lagoas: Embrapa Milho e Sorgo, 2010. (Embrapa Milho e Sorgo. Sistema de produção, 2).Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Milho e Sorgo. |
| |
10. | | NETTO, D. A. M.; MENDES, M. D. L.; COELHO, R. R.; RIBEIRO, P. E. de A.; MARION, M.; SITTON, M. Acreditação de ensaios de laboratório de análise de sementes da Embrapa Milho e Sorgo pela ISO/IEC 17025:2005. In: CONGRESSO DA ASSOCIAÇÃO BRASILEIRA DE PESQUISA TECNOLÓGICA, 2008, Campina Grande. Resumos. Brasília, DF: ABIPTI, 2008. p. 42.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
| |
12. | | NETTO, D. A. M.; MENDES, M. D. L.; COELHO, R. R.; RIBEIRO, P. E. de A.; MARION, M. L. P. Acreditação do laboratório de análise de sementes da Embrapa Milho e Sorgo pela ISO 17025. In: CONGRESSO NACIONAL DE MILHO E SORGO, 27.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 3.; WORKSHOP SOBRE MANEJO E ETIOLOGIA DA MANCHA BRANCA DO MILHO, 2008, Londrina. Agroenergia, produção de alimentos e mudanças climáticas: desafios para milho e sorgo: trabalhos e palestras. [Londrina]: IAPAR; [Sete Lagoas]: Embrapa Milho e Sorgo, 2008. 1 CD-ROM.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Milho e Sorgo. |
| |
15. | | LACERDA, A. F.; COELHO, R. R.; SILVA, M. S.; ROCHA, T. L.; GROSSI DE SÁ, M. F. A recombinant plant defensin highly effective towards. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 2., 2009, Búzios, RJ. Programa de resumos... [Ribeirão Preto]: Sociedade Brasileira de Genética, 2009. p. 232Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados. |
| |
17. | | MIRANDA, V. J.; FRAGOSO, R. da R.; VIANA, A. A. B.; OLIVEIRA NETO, O. B.; COELHO, R. R.; CARNEIRO, R. M. D. G.; SA, M. F. G. de. Análise da expressão gênica da enzima de conjugação a ubiquitina (e2) em soja em resposta a estresse biótico. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 008.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
19. | | NETTO, D. A. M.; MENDES, M. D. L.; COELHO, R. R.; RIBEIRO, P. E. de A.; MARION, M. L. P.; SITTON, M. Acreditação de ensaios do laboratório de análises de sementes da Embrapa Milho e Sorgo pelo ISO/IEC 17025:2005. In: CONGRESSO DA ASSOCIAÇÃO BRASILEIRA DE INSTITUIÇÕES DE PESQUISA TECNOLÓGICA, 2008, Campina Grande. Os desníveis regionais e a inovação no Brasil: os desafios para instituições tecnológica: anais. Brasília,, DF: ABIPTI, 2008. 10p. CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Meio Ambiente. |
| |
20. | | LIMA, R. F. C.; SOUZA JUNIOR, J. D. de; COELHO, R. R.; BARBOSA, A.; SA, M. F. G. de; REIS, A. M. dos. Clonagem do gene superóxido dismutase (sod) a partir de biblioteca de cDNA de raiz de algodão para superexpressão em tabaco. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 023.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
Registros recuperados : 46 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|