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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/12/2007 |
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Data da última atualização: |
25/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, M. R. R.; MOTA, F. F. da; CARNEIRO, N. P.; MARRIEL, I. E.; PAIVA, E.; ROSADO, A. S.; SELDIN, L. |
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Afiliação: |
Marcia Reed Rodrigues Coelho, UFRJ; Fabio Faria da Mota, UFRJ; NEWTON PORTILHO CARNEIRO, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; EDILSON PAIVA, CNPMS; Alexandre Soares Rosado, UFRJ; Lucy Seldin, UFRJ. |
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Título: |
Diversity of Paenibacillus spp. in the rhizosphere of four sorghum (Sorghum bicolor) cultivars sown with two contrasting levels of nitrogen fertilizer assessed by rpoB-Based PCR-DGGE and sequencing analysis. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Journal of Microbiology and Biotechnology, v. 17, n. 5, p. 753-760, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
The diversity of Paenibacillus species was assessed in the rhizospheres of four cultivars of sorghum sown in Cerrado soil amended with two level of nitrogen fertilizer (12 and 120 kg/ha). Two cuItivars (IS 5322.C and IS 6320) demanded the higher amount of nitrogen to grow, whereas the other two (FBS 8701.9 and IPA 1011) did not. Using the DNA extracted from the rhizospheres, a Paenibacillus-specific PCR system based ou the RNA polymerase gene (rpoB) was chosen for the molecular analyses. The resulting PCR products were separated into çommunity fingerprints by DGGE and the results showed a clear distinction between cultivars. In addition, clone libraries were generated from the rpoB fragments of two cultivars (IPA 1011 and IS 5322.C) using both fertilization conditions, and 318 selected clones were sequenced. Analyzed sequences were grouped into 14 Paenibacillus species. A greater diversity of Paenibacillus species was observed in cultivar IPA 1011 compared with cultivar IS 5322.C, Moreover, statistical analyses of the sequences showed that the bacterial diversity was more influenced by cultivar type than nitrogen fertilízation, corroborating the DGGE results. Thus, the sorghum cultivar type was the oveniding determiinative factor that influenced the community structures of the Paenibacillus communities in the habitats investigated. |
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Palavras-Chave: |
Molecular ecology; rpoB gene. |
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Thesagro: |
Sorghum Bicolor. |
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Thesaurus Nal: |
nitrogen fixation; Paenibacillus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02218naa a2200253 a 4500
001 1490073
005 2018-05-25
008 2007 bl uuuu u00u1 u #d
100 1 $aCOELHO, M. R. R.
245 $aDiversity of Paenibacillus spp. in the rhizosphere of four sorghum (Sorghum bicolor) cultivars sown with two contrasting levels of nitrogen fertilizer assessed by rpoB-Based PCR-DGGE and sequencing analysis.$h[electronic resource]
260 $c2007
520 $aThe diversity of Paenibacillus species was assessed in the rhizospheres of four cultivars of sorghum sown in Cerrado soil amended with two level of nitrogen fertilizer (12 and 120 kg/ha). Two cuItivars (IS 5322.C and IS 6320) demanded the higher amount of nitrogen to grow, whereas the other two (FBS 8701.9 and IPA 1011) did not. Using the DNA extracted from the rhizospheres, a Paenibacillus-specific PCR system based ou the RNA polymerase gene (rpoB) was chosen for the molecular analyses. The resulting PCR products were separated into çommunity fingerprints by DGGE and the results showed a clear distinction between cultivars. In addition, clone libraries were generated from the rpoB fragments of two cultivars (IPA 1011 and IS 5322.C) using both fertilization conditions, and 318 selected clones were sequenced. Analyzed sequences were grouped into 14 Paenibacillus species. A greater diversity of Paenibacillus species was observed in cultivar IPA 1011 compared with cultivar IS 5322.C, Moreover, statistical analyses of the sequences showed that the bacterial diversity was more influenced by cultivar type than nitrogen fertilízation, corroborating the DGGE results. Thus, the sorghum cultivar type was the oveniding determiinative factor that influenced the community structures of the Paenibacillus communities in the habitats investigated.
650 $anitrogen fixation
650 $aPaenibacillus
650 $aSorghum Bicolor
653 $aMolecular ecology
653 $arpoB gene
700 1 $aMOTA, F. F. da
700 1 $aCARNEIRO, N. P.
700 1 $aMARRIEL, I. E.
700 1 $aPAIVA, E.
700 1 $aROSADO, A. S.
700 1 $aSELDIN, L.
773 $tJournal of Microbiology and Biotechnology$gv. 17, n. 5, p. 753-760, 2007.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Origem |
Tipo/Formato |
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Registro |
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URL |
Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
21/06/2017 |
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Data da última atualização: |
04/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
DANTAS, B. F.; ANGELOTTI, F.; ARAGÃO, C. A. |
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Afiliação: |
BARBARA FRANCA DANTAS, CPATSA; FRANCISLENE ANGELOTTI, CPATSA; CARLOS ALBERTO ARAGÃO, Professor da Universidade do Estado da Bahia-UNEB, Juazeiro, Bahia. |
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Título: |
Will increasing temperature and CO2 affect pumpkin early development in Brazilian Semi-Arid? |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Revista Geama, Recife, v. 9, n. 1, p. 51-57, abr./jun. 2017. |
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ISSN: |
2447-0740 |
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Idioma: |
Inglês |
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Conteúdo: |
With rising levels of CO2 in atmosphere, understanding possible impacts on development and growth of plants becomes increasingly important. The aim of this study was to evaluate interaction between different temperatures and CO2 levels in germination and early development of seedlings of different species of pumpkin. Seeds of Cucurbita pepo cultivars ?Caserta? and ?Redonda?, and Cucurbita maxima ?Coroa? were sown in trays of 36 cells and held in growth chambers with different combinations of levels of CO2 and day/night temperatures. The experimental design was completely randomized in a 2 X 3 factorial scheme with two levels of CO2 concentration (360 and 550ppm) and three day/night temperatures (26/20, 29/26 and 32/26°C), with four replicates of 18 seedlings for each treatment. CO2 levels used caused different effects among cultivars for most variables, but a significant change in physiological behavior of seedlings with increasing CO2 concentration was not observed. Increase in temperature led to physiological changes in both seeds and seedlings. The predicted conditions of increasing concentration of atmospheric CO2 and temperature are damaging to production of pumpkin seedlings |
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Palavras-Chave: |
CO2; Cucurbitácea; Moranga; Mudanças Climáticas; Pumpkin. |
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Thesagro: |
Abóbora; Cucurbitaceae; Etapa de Desenvolvimento da Planta; Germinação; Hortaliça. |
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Thesaurus NAL: |
Climate change; Germination. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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Marc: |
LEADER 02043naa a2200301 a 4500
001 2071186
005 2018-01-04
008 2017 bl uuuu u00u1 u #d
022 $a2447-0740
100 1 $aDANTAS, B. F.
245 $aWill increasing temperature and CO2 affect pumpkin early development in Brazilian Semi-Arid?$h[electronic resource]
260 $c2017
520 $aWith rising levels of CO2 in atmosphere, understanding possible impacts on development and growth of plants becomes increasingly important. The aim of this study was to evaluate interaction between different temperatures and CO2 levels in germination and early development of seedlings of different species of pumpkin. Seeds of Cucurbita pepo cultivars ?Caserta? and ?Redonda?, and Cucurbita maxima ?Coroa? were sown in trays of 36 cells and held in growth chambers with different combinations of levels of CO2 and day/night temperatures. The experimental design was completely randomized in a 2 X 3 factorial scheme with two levels of CO2 concentration (360 and 550ppm) and three day/night temperatures (26/20, 29/26 and 32/26°C), with four replicates of 18 seedlings for each treatment. CO2 levels used caused different effects among cultivars for most variables, but a significant change in physiological behavior of seedlings with increasing CO2 concentration was not observed. Increase in temperature led to physiological changes in both seeds and seedlings. The predicted conditions of increasing concentration of atmospheric CO2 and temperature are damaging to production of pumpkin seedlings
650 $aClimate change
650 $aGermination
650 $aAbóbora
650 $aCucurbitaceae
650 $aEtapa de Desenvolvimento da Planta
650 $aGerminação
650 $aHortaliça
653 $aCO2
653 $aCucurbitácea
653 $aMoranga
653 $aMudanças Climáticas
653 $aPumpkin
700 1 $aANGELOTTI, F.
700 1 $aARAGÃO, C. A.
773 $tRevista Geama, Recife$gv. 9, n. 1, p. 51-57, abr./jun. 2017.
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