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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
04/05/2020 |
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Data da última atualização: |
04/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZUCCHI, M. I.; BRONDANI, R. P. V.; PINHEIRO, J. B.; CHAVES, L. J.; COELHO, A. S. G.; VENCOVSKY, R. |
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Afiliação: |
MARIA IMACULADA ZUCCHI, ESALQ; ROSANA PEREIRA VIANELLO, CNPAF; JOSE BALDIN PINHEIRO, UFG; LAZARO JOSE CHAVES, UFG; ALEXANDRE SIQUEIRA GUEDES COELHO, UFG; ROLAND VENCOVSKY, ESALQ. |
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Título: |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 26, n. 4, p. 449-457, dez. 2003. |
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ISSN: |
1678-4685 |
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DOI: |
https://doi.org/10.1590/S1415-47572003000400008 |
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Idioma: |
Inglês |
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Conteúdo: |
The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, RST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure. |
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Palavras-Chave: |
Genetic diversity; SSR; Tropical tree. |
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Thesagro: |
Cagaita; Cerrado; Eugenia Dysenterica; Marcador Molecular; Myrtaceae; Variação Genética. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212701/1/CNPAF-2003-gmb.pdf
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Marc: |
LEADER 02280naa a2200313 a 4500
001 2122034
005 2022-05-04
008 2003 bl uuuu u00u1 u #d
022 $a1678-4685
024 7 $ahttps://doi.org/10.1590/S1415-47572003000400008$2DOI
100 1 $aZUCCHI, M. I.
245 $aGenetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers.$h[electronic resource]
260 $c2003
520 $aThe ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, RST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure.
650 $aCagaita
650 $aCerrado
650 $aEugenia Dysenterica
650 $aMarcador Molecular
650 $aMyrtaceae
650 $aVariação Genética
653 $aGenetic diversity
653 $aSSR
653 $aTropical tree
700 1 $aBRONDANI, R. P. V.
700 1 $aPINHEIRO, J. B.
700 1 $aCHAVES, L. J.
700 1 $aCOELHO, A. S. G.
700 1 $aVENCOVSKY, R.
773 $tGenetics and Molecular Biology$gv. 26, n. 4, p. 449-457, dez. 2003.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
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Data corrente: |
22/08/2022 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
NICIURA, S. C. M.; BENAVIDES, M. V.; OKINO, C. H.; IBELLI, A. M. G.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; CINTIA HIROMI OKINO, CPPSE; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; ALESSANDRO PELEGRINE MINHO, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Genome-wide association study for Haemonchus contortus resistance in Morada Nova sheep. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pathogens, v. 11, n. 8, aug. 2022, 939. |
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Páginas: |
12 p. |
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DOI: |
https://doi.org/10.3390/pathogens11080939 |
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Idioma: |
Inglês |
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Conteúdo: |
Among the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification. MenosAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for... Mostrar Tudo |
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Palavras-Chave: |
Gastrointestinal nematode control; GWAS; Molecular markers; Ovine; Parasite resistance. |
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Thesagro: |
Marcador Molecular; Ovino; Parasito. |
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Thesaurus NAL: |
Genotyping. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145606/1/GenomeWideAssociation.pdf
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Marc: |
LEADER 02449naa a2200325 a 4500
001 2145606
005 2022-08-22
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/pathogens11080939$2DOI
100 1 $aNICIURA, S. C. M.
245 $aGenome-wide association study for Haemonchus contortus resistance in Morada Nova sheep.$h[electronic resource]
260 $c2022
300 $a12 p.
520 $aAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification.
650 $aGenotyping
650 $aMarcador Molecular
650 $aOvino
650 $aParasito
653 $aGastrointestinal nematode control
653 $aGWAS
653 $aMolecular markers
653 $aOvine
653 $aParasite resistance
700 1 $aBENAVIDES, M. V.
700 1 $aOKINO, C. H.
700 1 $aIBELLI, A. M. G.
700 1 $aMINHO, A. P.
700 1 $aESTEVES, S. N.
700 1 $aCHAGAS, A. C. de S.
773 $tPathogens$gv. 11, n. 8, aug. 2022, 939.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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