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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Instrumentação. |
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Data corrente: |
28/09/2022 |
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Data da última atualização: |
28/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COATRINI-SOARES, A.; COATRINI-SOARES, J.; POPOLIN NETO, M.; MELLO, S. S. de; PINTO, D. S. C.; CARVALHO, W. A.; GILMORE, M. S.; PIAZZETTA, M. H. O.; GOBBI, A. L.; BRANDÃO, H. M.; PAULOVICH, F. V.; OLIVEIRA JR, O. N.; MATTOSO, L. H. C. |
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Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Microfluidic E-tongue to diagnose bovine mastitis with milk samples using machine learning with decision tree models. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Chemical Engineering Journal, v. 451, e138523, 2022. |
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Páginas: |
1 - 9 |
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ISSN: |
1385-8947 |
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DOI: |
https://doi.org/10.1016/j.cej.2022.138523 |
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Idioma: |
Inglês |
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Conteúdo: |
We report an electronic tongue based on impedance spectroscopy to detect Staphylococcus aureus and diagnose bovine mastitis in milk samples. This was achieved with optimized sensing units made with layer-by-layer films and by treating the capacitance data with machine learning algorithms employing decision trees models. These films were made with chitosan, chondroitin sulfate, sericin and gold nanoparticles /sericin, whose molecularlevel interaction with S.aureus depended on the architecture according to PM-IRRAS measurements. The limit of detection in blank milk varied from 3.41 to 2.01 CFU/mL depending on the sensing unit. This sensitivity was complemented with the selectivity provided by combining the electrical responses of the four sensing units. Indeed, with machine learning it was possible to determine multidimensional calibration spaces (MCS) that could generate rules to explain how the milk samples could be discriminated. With a 7-dimension MCS, distinct S. aureus concentrations could be distinguished from possible interferents with a 100 % accuracy. In crude milk samples, 94 % accuracy was obtained with a 6-dimension MCS in multiclass classification for milk from different udders of a mastitis infected cow, including samples diluted 50-fold, in addition to milk from an infected cow treated with Bronopol and from a healthy cow. It is significant that in a ternary classification with these crude milk samples, a 2-dimension MCS could distinguish between milk from an infected cow, treated with Bronopol and from a healthy cow with 100 % accuracy. The combination of electronic tongues and machine learning ? as in this proof-of-concept study - is promising for diagnosis of mastitis at a low cost. MenosWe report an electronic tongue based on impedance spectroscopy to detect Staphylococcus aureus and diagnose bovine mastitis in milk samples. This was achieved with optimized sensing units made with layer-by-layer films and by treating the capacitance data with machine learning algorithms employing decision trees models. These films were made with chitosan, chondroitin sulfate, sericin and gold nanoparticles /sericin, whose molecularlevel interaction with S.aureus depended on the architecture according to PM-IRRAS measurements. The limit of detection in blank milk varied from 3.41 to 2.01 CFU/mL depending on the sensing unit. This sensitivity was complemented with the selectivity provided by combining the electrical responses of the four sensing units. Indeed, with machine learning it was possible to determine multidimensional calibration spaces (MCS) that could generate rules to explain how the milk samples could be discriminated. With a 7-dimension MCS, distinct S. aureus concentrations could be distinguished from possible interferents with a 100 % accuracy. In crude milk samples, 94 % accuracy was obtained with a 6-dimension MCS in multiclass classification for milk from different udders of a mastitis infected cow, including samples diluted 50-fold, in addition to milk from an infected cow treated with Bronopol and from a healthy cow. It is significant that in a ternary classification with these crude milk samples, a 2-dimension MCS could distinguish between milk from an i... Mostrar Tudo |
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Palavras-Chave: |
Impedance spectroscopy; Machine learning; Mastite; Multidimensional calibration space; S aureus; Sensors. |
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Thesagro: |
Bovino; Doença Animal; Gado Leiteiro. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02936naa a2200409 a 4500
001 2146958
005 2022-09-28
008 2022 bl uuuu u00u1 u #d
022 $a1385-8947
024 7 $ahttps://doi.org/10.1016/j.cej.2022.138523$2DOI
100 1 $aCOATRINI-SOARES, A.
245 $aMicrofluidic E-tongue to diagnose bovine mastitis with milk samples using machine learning with decision tree models.$h[electronic resource]
260 $c2022
300 $a1 - 9
520 $aWe report an electronic tongue based on impedance spectroscopy to detect Staphylococcus aureus and diagnose bovine mastitis in milk samples. This was achieved with optimized sensing units made with layer-by-layer films and by treating the capacitance data with machine learning algorithms employing decision trees models. These films were made with chitosan, chondroitin sulfate, sericin and gold nanoparticles /sericin, whose molecularlevel interaction with S.aureus depended on the architecture according to PM-IRRAS measurements. The limit of detection in blank milk varied from 3.41 to 2.01 CFU/mL depending on the sensing unit. This sensitivity was complemented with the selectivity provided by combining the electrical responses of the four sensing units. Indeed, with machine learning it was possible to determine multidimensional calibration spaces (MCS) that could generate rules to explain how the milk samples could be discriminated. With a 7-dimension MCS, distinct S. aureus concentrations could be distinguished from possible interferents with a 100 % accuracy. In crude milk samples, 94 % accuracy was obtained with a 6-dimension MCS in multiclass classification for milk from different udders of a mastitis infected cow, including samples diluted 50-fold, in addition to milk from an infected cow treated with Bronopol and from a healthy cow. It is significant that in a ternary classification with these crude milk samples, a 2-dimension MCS could distinguish between milk from an infected cow, treated with Bronopol and from a healthy cow with 100 % accuracy. The combination of electronic tongues and machine learning ? as in this proof-of-concept study - is promising for diagnosis of mastitis at a low cost.
650 $aBovino
650 $aDoença Animal
650 $aGado Leiteiro
653 $aImpedance spectroscopy
653 $aMachine learning
653 $aMastite
653 $aMultidimensional calibration space
653 $aS aureus
653 $aSensors
700 1 $aCOATRINI-SOARES, J.
700 1 $aPOPOLIN NETO, M.
700 1 $aMELLO, S. S. de
700 1 $aPINTO, D. S. C.
700 1 $aCARVALHO, W. A.
700 1 $aGILMORE, M. S.
700 1 $aPIAZZETTA, M. H. O.
700 1 $aGOBBI, A. L.
700 1 $aBRANDÃO, H. M.
700 1 $aPAULOVICH, F. V.
700 1 $aOLIVEIRA JR, O. N.
700 1 $aMATTOSO, L. H. C.
773 $tChemical Engineering Journal$gv. 451, e138523, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Biblioteca |
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Tipo/Formato |
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Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
21/09/2018 |
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Data da última atualização: |
05/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ARAÚJO, R. A. de; NEIVA, J. N. M.; POMPEU, R. C. F. F.; CÂNDIDO, M. J. D.; ROGERIO, M. C. P.; LUCAS, R. C.; MARANHÃO, S. R.; FONTINELE, R. G.; EGITO, A. S. do. |
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Afiliação: |
Ricardo Alves de Araújo, Pós-graduação integrada - UFC/UFRPE/UFPB - Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brasil.; José Neuman Miranda Neiva, Universidade Federal do Tocantins (UFT) - Araguaína, TO, Brasil; ROBERTO CLAUDIO FERNANDES F POMPEU, CNPC; Magno José Duarte Cândido, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brasil; MARCOS CLAUDIO PINHEIRO ROGERIO, CNPC; Ronaldo Carlos Lucas, Pós-graduação - Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brasil.; Samuel Rocha Maranhão, Pós-graduação integrada - UFC/UFRPE/UFPB - Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brasil.; Renato Gomes Fontinele; ANTONIO SILVIO DO EGITO, CNPC. |
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Título: |
Feeding behavior and physiological parameters of rearing goats fed diets containing detoxified castor cake. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 39, n. 5, p. 2247-2260, set./out. 2018. |
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DOI: |
10.5433/1679-0359.2018v39n5p2247 |
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Idioma: |
Português |
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Conteúdo: |
Abstract: The objective of this study was to evaluate the influence of replacing soybean meal (SM) with castor bean cake (DCC), detoxified using two alkaline products, on the feeding behavior and physiological parameters of Saanen and Anglo-Nubian goat breeds with an initial body weight of 16.22 ± 0.67 kg. The treatments consisted of three diets that were isoproteic and isoenergetic: the first with a standard formulation based on corn and SM, the second with DCC detoxified using calcium hydroxide (Ca(OH)2 DCC), and the third with DCC detoxified using sodium hydroxide (NaOH DCC). A completely randomized design with a 3 × 2 (diets and breeds) factorial arrangement and four replicates per combination was used. A dietary effect on the intake of dry matter (DM) and NDFap (P = 0.02 and 0.01) was observed, with lower values for goats fed NaOH DCC than the other diets. The diets influenced feeding and rumination times (P = 0.04 and 0.03). An interaction (P = 0.04 and 0.01) between the breeds and the SM-based diet was found for time variables (other activity and idle time). Increased feeding and neutral detergent fiber (NDF) rumination efficiencies were observed for goats fed SM and Ca(OH)2 DCC (P = 0.03 and 0.01). Goats fed the NaOH DCC diet consumed large amounts of water, while Saanen goats urinated more times than did Anglo-Nubian goats. The diets had an effect on the heart rate and rectal temperature (P = 0.01 and 0.02), while the body surface temperature was influenced by the breed. Thus, Ca(OH)2 or NaOH DCC has a great potential to replace SM in the feeding of dairy goats. When used as diet for goats in the growth phase, NaOH DCC causes a reduction of DM and NDF intakes. Neither DCC negatively affected the physiological parameters of goats. [Comportamento alimentar e parâmetros fisiológicos de cabritas recriadas consumindo dietas contendo torta de mamona destoxificada]. Resumo: Objetivou-se avaliar a influência da substituição do farelo de soja (FS) pela torta de mamona destoxificada (TMD) por dois produtos alcalinos sobre o comportamento alimentar e parâmetros fisiológicos de cabritas das raças Saanen e Anglo-nubiana, com peso corporal inicial de 16,22 ± 0,67 kg. Os tratamentos consistiram de três dietas, uma com formulação padrão à base de milho e FS e as demais foram formuladas com torta de mamona destoxificada por hidróxido de cálcio (TMD Ca(OH)2 ) e outra por hidróxido de sódio (TMD NaOH), as quais foram isoproteicas e isoenergéticas. Utilizouse um delineamento inteiramente casualizado, com arranjo fatorial 3 x 2 (dieta e raça) com quatro repetições por combinação. Houve efeito das dietas sobre o consumo de MS e FDNcp (P= 0,02 e 0,01), com menores valores para as cabras alimentadas com TMD NaOH. As dietas influenciaram os tempos de alimentação e ruminação (P= 0,04 e 0,03). Houve interação (P= 0,04 e 0,01) entre as raças e dieta à base de FS para as variáveis tempo com outras atividade e ócio. Observou-se maiores eficiências de alimentação e ruminação da FDN para as cabras que consumiram FS e TMD Ca(OH)2 (P= 0,03 e 0,01). As cabras da dieta TMD NaOH consumiram maiores quantidades de água e as Saanen urinaram mais vezes que as Anglo-nubiana. Houve efeito das dietas sobre a frequência cardíaca e temperatura retal (P= 0,01 e 0,02), já a temperatura corporal foi influenciada pelas raças. A torta de mamona destoxificada pelo hidróxido de cálcio e/ou hidróxido de sódio tem grande potencial para substituir o farelo de soja na alimentação de cabras leiteiras. Quando usado na composição de dietas para caprinos em fase de crescimento, a torta de mamona destoxificada pelo hidróxido de sódio leva à redução do CMS, bem como da FDN. Ambas as tortas não alteram negativamente os parâmetros fisiológicos das cabras.. MenosAbstract: The objective of this study was to evaluate the influence of replacing soybean meal (SM) with castor bean cake (DCC), detoxified using two alkaline products, on the feeding behavior and physiological parameters of Saanen and Anglo-Nubian goat breeds with an initial body weight of 16.22 ± 0.67 kg. The treatments consisted of three diets that were isoproteic and isoenergetic: the first with a standard formulation based on corn and SM, the second with DCC detoxified using calcium hydroxide (Ca(OH)2 DCC), and the third with DCC detoxified using sodium hydroxide (NaOH DCC). A completely randomized design with a 3 × 2 (diets and breeds) factorial arrangement and four replicates per combination was used. A dietary effect on the intake of dry matter (DM) and NDFap (P = 0.02 and 0.01) was observed, with lower values for goats fed NaOH DCC than the other diets. The diets influenced feeding and rumination times (P = 0.04 and 0.03). An interaction (P = 0.04 and 0.01) between the breeds and the SM-based diet was found for time variables (other activity and idle time). Increased feeding and neutral detergent fiber (NDF) rumination efficiencies were observed for goats fed SM and Ca(OH)2 DCC (P = 0.03 and 0.01). Goats fed the NaOH DCC diet consumed large amounts of water, while Saanen goats urinated more times than did Anglo-Nubian goats. The diets had an effect on the heart rate and rectal temperature (P = 0.01 and 0.02), while the body surface temperature was influenced by the b... Mostrar Tudo |
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Palavras-Chave: |
Caprino leiteiro; Feed conversion efficiency; Raça Anglo-Nubiana; Raça Saanen; Torta de mamona. |
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Thesagro: |
Água; Alimento Para Animal; Cabra Leiteira; Cálcio; Dieta; Farelo; Habito Alimentar; Nutrição Animal; Ricinus Communis; Ruminação; Sódio; Subproduto. |
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Thesaurus NAL: |
Animal behavior; Animal feeders; Animal nutrition; Brazil; Byproducts; Consumer behavior; Dairy goats; Diet; Feeding behavior; Semiarid zones; Water. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183320/1/cnpc-2018-Feeding.pdf
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Marc: |
LEADER 05414naa a2200565 a 4500
001 2096106
005 2019-04-05
008 2018 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2018v39n5p2247$2DOI
100 1 $aARAÚJO, R. A. de
245 $aFeeding behavior and physiological parameters of rearing goats fed diets containing detoxified castor cake.$h[electronic resource]
260 $c2018
520 $aAbstract: The objective of this study was to evaluate the influence of replacing soybean meal (SM) with castor bean cake (DCC), detoxified using two alkaline products, on the feeding behavior and physiological parameters of Saanen and Anglo-Nubian goat breeds with an initial body weight of 16.22 ± 0.67 kg. The treatments consisted of three diets that were isoproteic and isoenergetic: the first with a standard formulation based on corn and SM, the second with DCC detoxified using calcium hydroxide (Ca(OH)2 DCC), and the third with DCC detoxified using sodium hydroxide (NaOH DCC). A completely randomized design with a 3 × 2 (diets and breeds) factorial arrangement and four replicates per combination was used. A dietary effect on the intake of dry matter (DM) and NDFap (P = 0.02 and 0.01) was observed, with lower values for goats fed NaOH DCC than the other diets. The diets influenced feeding and rumination times (P = 0.04 and 0.03). An interaction (P = 0.04 and 0.01) between the breeds and the SM-based diet was found for time variables (other activity and idle time). Increased feeding and neutral detergent fiber (NDF) rumination efficiencies were observed for goats fed SM and Ca(OH)2 DCC (P = 0.03 and 0.01). Goats fed the NaOH DCC diet consumed large amounts of water, while Saanen goats urinated more times than did Anglo-Nubian goats. The diets had an effect on the heart rate and rectal temperature (P = 0.01 and 0.02), while the body surface temperature was influenced by the breed. Thus, Ca(OH)2 or NaOH DCC has a great potential to replace SM in the feeding of dairy goats. When used as diet for goats in the growth phase, NaOH DCC causes a reduction of DM and NDF intakes. Neither DCC negatively affected the physiological parameters of goats. [Comportamento alimentar e parâmetros fisiológicos de cabritas recriadas consumindo dietas contendo torta de mamona destoxificada]. Resumo: Objetivou-se avaliar a influência da substituição do farelo de soja (FS) pela torta de mamona destoxificada (TMD) por dois produtos alcalinos sobre o comportamento alimentar e parâmetros fisiológicos de cabritas das raças Saanen e Anglo-nubiana, com peso corporal inicial de 16,22 ± 0,67 kg. Os tratamentos consistiram de três dietas, uma com formulação padrão à base de milho e FS e as demais foram formuladas com torta de mamona destoxificada por hidróxido de cálcio (TMD Ca(OH)2 ) e outra por hidróxido de sódio (TMD NaOH), as quais foram isoproteicas e isoenergéticas. Utilizouse um delineamento inteiramente casualizado, com arranjo fatorial 3 x 2 (dieta e raça) com quatro repetições por combinação. Houve efeito das dietas sobre o consumo de MS e FDNcp (P= 0,02 e 0,01), com menores valores para as cabras alimentadas com TMD NaOH. As dietas influenciaram os tempos de alimentação e ruminação (P= 0,04 e 0,03). Houve interação (P= 0,04 e 0,01) entre as raças e dieta à base de FS para as variáveis tempo com outras atividade e ócio. Observou-se maiores eficiências de alimentação e ruminação da FDN para as cabras que consumiram FS e TMD Ca(OH)2 (P= 0,03 e 0,01). As cabras da dieta TMD NaOH consumiram maiores quantidades de água e as Saanen urinaram mais vezes que as Anglo-nubiana. Houve efeito das dietas sobre a frequência cardíaca e temperatura retal (P= 0,01 e 0,02), já a temperatura corporal foi influenciada pelas raças. A torta de mamona destoxificada pelo hidróxido de cálcio e/ou hidróxido de sódio tem grande potencial para substituir o farelo de soja na alimentação de cabras leiteiras. Quando usado na composição de dietas para caprinos em fase de crescimento, a torta de mamona destoxificada pelo hidróxido de sódio leva à redução do CMS, bem como da FDN. Ambas as tortas não alteram negativamente os parâmetros fisiológicos das cabras..
650 $aAnimal behavior
650 $aAnimal feeders
650 $aAnimal nutrition
650 $aBrazil
650 $aByproducts
650 $aConsumer behavior
650 $aDairy goats
650 $aDiet
650 $aFeeding behavior
650 $aSemiarid zones
650 $aWater
650 $aÁgua
650 $aAlimento Para Animal
650 $aCabra Leiteira
650 $aCálcio
650 $aDieta
650 $aFarelo
650 $aHabito Alimentar
650 $aNutrição Animal
650 $aRicinus Communis
650 $aRuminação
650 $aSódio
650 $aSubproduto
653 $aCaprino leiteiro
653 $aFeed conversion efficiency
653 $aRaça Anglo-Nubiana
653 $aRaça Saanen
653 $aTorta de mamona
700 1 $aNEIVA, J. N. M.
700 1 $aPOMPEU, R. C. F. F.
700 1 $aCÂNDIDO, M. J. D.
700 1 $aROGERIO, M. C. P.
700 1 $aLUCAS, R. C.
700 1 $aMARANHÃO, S. R.
700 1 $aFONTINELE, R. G.
700 1 $aEGITO, A. S. do
773 $tSemina: Ciências Agrárias, Londrina$gv. 39, n. 5, p. 2247-2260, set./out. 2018.
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