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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
02/03/2016 |
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Data da última atualização: |
09/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, F. C.; CLEMENTE, A. da C. S.; CAIXETA, F.; ROSA, S. D. V. F. da. |
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Afiliação: |
UDI Pesquisa & Desenvolvimento; UFLA; General Mills Brasil Alimentos Ltda.; STTELA DELLYZETE VEIGA F DA ROSA, SAPC. |
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Título: |
Endo-ß-mannanase and ß-tubulin gene expression during the final phases of coffee seed maturation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 4, p. 11719-11728, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee seed maturation. MenosCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee se... Mostrar Tudo |
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Palavras-Chave: |
QRT-PCR. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Developmental stages. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140475/1/Endo-B-mannanase.pdf
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Marc: |
LEADER 02122naa a2200193 a 4500
001 2039275
005 2019-05-09
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C.
245 $aEndo-ß-mannanase and ß-tubulin gene expression during the final phases of coffee seed maturation.$h[electronic resource]
260 $c2015
520 $aCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee seed maturation.
650 $aDevelopmental stages
650 $aCoffea Arábica
653 $aQRT-PCR
700 1 $aCLEMENTE, A. da C. S.
700 1 $aCAIXETA, F.
700 1 $aROSA, S. D. V. F. da
773 $tGenetics and Molecular Research$gv. 14, n. 4, p. 11719-11728, 2015.
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Registro original: |
Embrapa Café (CNPCa) |
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| 101. |  | SOARES, D. M.; THUNG, M.; AIDAR, H.; KLUTHCOUSKI, J. Estimativa de custo de produção de feijão: coeficientes técnicos, custos, rendimentos e rentabiidade. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 8., 2005, Goiânia. Anais... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2005. v. 2 p. 881-883. (Embrapa Arroz e Feijão. Documentos, 182).| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 110. | | KLUTHCOUSKI, J.; AIDAR, H.; THUNG, M.; COBUCCI, T. Manejo antecipado do nitrogênio na cultura do arroz de terras altas com restrições de umidade no solo. In: CONGRESSO BRASILEIRO DA CADEIA PRODUTIVA DE ARROZ, 2.; REUNIÃO NACIONAL DE PESQUISA DE ARROZ, 8., 2006, Brasília, DF. Anais... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2006. (Embrapa Arroz e Feijão. Documentos, 196).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 111. | | AIDAR, H.; SOARES, D. M.; THUNG, M.; KLUTHCOUSKI, J. Manejo antecipado do nitrogênio na cultura do arroz de terras altas sem restrições de umidade no solo. In: CONGRESSO BRASILEIRO DA CADEIA PRODUTIVA DE ARROZ, 2.; REUNIÃO NACIONAL DE PESQUISA DE ARROZ, 8., 2006, Brasília, DF. Anais... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2006. (Embrapa Arroz e Feijão. Documentos, 196).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 112. | | ALVARENGA, R. C.; GONTIJO NETO, M. M.; KLUTHCOUSKI, J. O milho na integração lavoura-pecuária. In: CRUZ, J. C.; MAGALHAES, P. C.; PEREIRA FILHO, I. A.; MOREIRA, J. A. A. (Ed.). Milho: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa Informação Tecnológica; Sete Lagoas: Embrapa Milho e Sorgo, 2011. cap. 17, p. 269-278. (Coleção 500 perguntas, 500 respostas).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Milho e Sorgo. |
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| Registros recuperados : 344 | |
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