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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/06/1995 |
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Data da última atualização: |
27/06/1995 |
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Autoria: |
MARTEL, J. H. I.; CLEMENT, C. R. |
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Título: |
Comparacao preliminar da area foliar de tres acessos de pupunha (Bactris gasipaes H.B.K., Palmae) oriundos de tres populacoes distintas da Amazonia Ocidental. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Acta Amazonica, v.16, p.13-18, 1986. |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00435naa a2200121 a 4500
001 1618222
005 1995-06-27
008 1986 bl --- 0-- u #d
100 1 $aMARTEL, J. H. I.
245 $aComparacao preliminar da area foliar de tres acessos de pupunha (Bactris gasipaes H.B.K., Palmae) oriundos de tres populacoes distintas da Amazonia Ocidental.
260 $c1986
700 1 $aCLEMENT, C. R.
773 $tActa Amazonica$gv.16, p.13-18, 1986.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
23/10/2009 |
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Data da última atualização: |
01/03/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
ANDREOTTI, R.; MATOS, M. de F. C.; GONÇALVES, K. N.; OSHIRO, L. M.; LIMA-JUNIOR, M. S. da C.; PAIVA, F.; LEITE, F. L. |
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Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; MARIA DE FÁTIMA CEPA MATOS, UFMS; KELLY NODA GONÇALVES, UFMS; LEANDRA MARLA OSHIRO, UFMS; MANOEL SEBASTIÃO DA COSTA LIMA-JUNIOR, UFMS; FERNANDO PAIVA, UFMS; FÁBIO LEIVAS LEITE, UFPel. |
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Título: |
Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Revista Brasileira de Parasitologia Veterinária, São Paulo, v.18, n.2, p.20-24, abr-jun. 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG
tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep. |
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Palavras-Chave: |
NcSRS2; Proteína recombinante; Teste. |
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Thesagro: |
Diagnostico; Elisa; Neospora Caninum; Neosporose; Ovino; Sanidade Animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02075naa a2200301 a 4500
001 1573041
005 2010-03-01
008 2009 bl --- 0-- u #d
100 1 $aANDREOTTI, R.
245 $aComparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep.
260 $c2009
520 $aNeospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep.
650 $aDiagnostico
650 $aElisa
650 $aNeospora Caninum
650 $aNeosporose
650 $aOvino
650 $aSanidade Animal
653 $aNcSRS2
653 $aProteína recombinante
653 $aTeste
700 1 $aMATOS, M. de F. C.
700 1 $aGONÇALVES, K. N.
700 1 $aOSHIRO, L. M.
700 1 $aLIMA-JUNIOR, M. S. da C.
700 1 $aPAIVA, F.
700 1 $aLEITE, F. L.
773 $tRevista Brasileira de Parasitologia Veterinária, São Paulo$gv.18, n.2, p.20-24, abr-jun. 2009.
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Embrapa Gado de Corte (CNPGC) |
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