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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
25/11/2002 |
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Data da última atualização: |
25/11/2002 |
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Autoria: |
BODENSTEINER, K. J.; CLAY, C. M.; MOELLER, C. L.; SAWYER, H. R. |
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Título: |
Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Biology of Reproduction, v. 60, n. 2, p. 381-386, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
Recently a novel member of the transforming growth factor Betha (TGFBetha) superfamily termed growth/differentiation factor-9 (GDF-9) was shown to be expressed in ovaries of mice and humans, and to be essential for normal follicular development beyond the primary (type 2) follicle stage in mice. In the present study, the gene for ovine GDF-9 was isolated and characterized, and expression of GDF-9 mRNA in ovaries of domestic ruminants was examined. The predicted amino acid sequence of ovine GDF-9 is 77% and 66% homologous to human and mouse GDF-9, respectively. Specific hybridization using homologous 35S-antisense probes was restricted to oocytes. In contrast to similar studies in mice in which GDF-9 was first detected beginning at the primary (type 2) follicle stage, in ovine and bovine ovaries GDF-9 mRNA was expressed beginning at the primordial (type 1) follicle stage. The observed timing and pattern of GDF-9 expression in oocytes of domestic ruminants is consistent with a role for GDF-9 in the initiation and maintenance of folliculogenesis in these species, and supports the general concept that early stages of follicular growth and development are regulated by intraovarian factors.
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Palavras-Chave: |
Clonagem molecular; Folículo ovariano. |
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Thesagro: |
Biologia Molecular; Bovino; Ovino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01873naa a2200217 a 4500
001 1528900
005 2002-11-25
008 1999 bl uuuu u00u1 u #d
100 1 $aBODENSTEINER, K. J.
245 $aMolecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries.
260 $c1999
520 $aRecently a novel member of the transforming growth factor Betha (TGFBetha) superfamily termed growth/differentiation factor-9 (GDF-9) was shown to be expressed in ovaries of mice and humans, and to be essential for normal follicular development beyond the primary (type 2) follicle stage in mice. In the present study, the gene for ovine GDF-9 was isolated and characterized, and expression of GDF-9 mRNA in ovaries of domestic ruminants was examined. The predicted amino acid sequence of ovine GDF-9 is 77% and 66% homologous to human and mouse GDF-9, respectively. Specific hybridization using homologous 35S-antisense probes was restricted to oocytes. In contrast to similar studies in mice in which GDF-9 was first detected beginning at the primary (type 2) follicle stage, in ovine and bovine ovaries GDF-9 mRNA was expressed beginning at the primordial (type 1) follicle stage. The observed timing and pattern of GDF-9 expression in oocytes of domestic ruminants is consistent with a role for GDF-9 in the initiation and maintenance of folliculogenesis in these species, and supports the general concept that early stages of follicular growth and development are regulated by intraovarian factors.
650 $aBiologia Molecular
650 $aBovino
650 $aOvino
653 $aClonagem molecular
653 $aFolículo ovariano
700 1 $aCLAY, C. M.
700 1 $aMOELLER, C. L.
700 1 $aSAWYER, H. R.
773 $tBiology of Reproduction$gv. 60, n. 2, p. 381-386, 1999.
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Embrapa Caprinos e Ovinos (CNPC) |
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