Data/hora: 09/06/2024 13:36:31

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Florestas; Embrapa Gado de Corte; Embrapa Trigo.
Data corrente: | 18/09/1997
Data da última atualização: | 10/07/1998
Autoria: | CLARK, M. S. (Ed.).
Título: | Plant molecular biology: a laboratory manual.
Ano de publicação: | 1997
Fonte/Imprenta: | Berlin: Springer-Verlag, 1997.
Páginas: | 529p.
ISBN: | 3-540-58405-6
Idioma: | Inglês
Conteúdo: | Basic molecular techniques. Genomic DNA isolation, southern blotting and hybrization.  Isolation of total genomic DNA Southern blotting. Hibridization with radioactive probes. Non-radioative methods of detection on southern blots. Cloning from genomic DNA and production of libraries. Use of PCRs in plant molecular biolog. Plasmid libraries. Lambda genomic cloning. Cosmid libraries.Yeast artifical chromosome (yac) libraries. Extration of RNA, cloning and subtractive hybridization. Isolation and analysis of messenge RNA form plant cells: cloning of cRNA from plant cells: cloning of cDNAs. Substractive hybrization of differnt mRNA populations. Characterization of clones. DNA sequencing. Expression of cloned gens. Characterization of plant DNA. Organelle DNA isolation. RAPD analysis: use for genome Characterization, tagging traits and mapping. RELP mapping of plant nuclear genomes: Planning of experiments, linkage map construction, and QTI mapping. Genetic engineering: methodology and analysis. Plant gene transfer. Molecular characterization of transformed plants. Molecular charactization of somatic hybrids. Cytological characterization of transformed plants. Molecular characterization of somatic hybrids. Cytological characterization of transformed plants.: mapping of low-copy and repetitive  DNA sequences by fluorescent in situ hybridization (FISH) . Cytological characterization of somatic hybrids: detection of genome origin by genomeic in situ hybrization (GISH). Addendum to chapters 11 and 12 in situ hybridization in  plant species with small chromosomes. Appendix nuclear genome sizes of important plant species.
Palavras-Chave: | Biology; Manual; Molecular; Plant; Plants; Tecnica.
Thesagro: | Biologia; Biologia Molecular; Biologia Vegetal; DNA; Engenharia; Engenharia Genética; Genética; Genética Molecular; Laboratório; Planta.
Thesaurus Nal: | botany; genetic engineering; laboratories; molecular biology; molecular genetics.
Categoria do assunto: | --
Registro original: | Embrapa Arroz e Feijão (CNPAF)

Exemplares:

CNPAF - (13803-1) - ADD - LV - -- - 572.8 - C594p - 1998.00074 - DIS
CNPF - (18896-1) - ADC - LV - -- - LV1360 -  - 1997.00223 - DIS
CNPGC - (11427-1) - ADC - LV - PP - 572.8 - C594p - 2006.00065 - DIS
CNPT - (7864-1) - ADC - LV - -- - 581.88 - C594p - 97.02501 - DIS