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Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
10/01/2018 |
Data da última atualização: |
10/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DOTTO, M.; PIROLA, K.; WAGNER JUNIOR, A.; RADAELLI, J. C.; ANTUNES, L. E. C.; CITADIN, I. |
Afiliação: |
Marcelo Dotto; Kelli Pirola; Américo Wagner Junior; Juliana Cristina Radaelli; LUIS EDUARDO CORREA ANTUNES, CPACT. |
Título: |
Physiological and sensorial aspects of peach fruits cv. Chimarrita depending on the season and management of green pruning. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Brazilian Journal of Agriculture, v. 92, n. 3, p. 261-270, 2017. |
Idioma: |
Inglês |
Palavras-Chave: |
Cultural practice. |
Thesagro: |
Prunus Persica. |
Thesaurus Nal: |
fruits. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170739/1/Luis-Eduardo-PODA-VERDE-CHIMARRITA.pdf
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Marc: |
LEADER 00656naa a2200205 a 4500 001 2084843 005 2018-01-10 008 2017 bl uuuu u00u1 u #d 100 1 $aDOTTO, M. 245 $aPhysiological and sensorial aspects of peach fruits cv. Chimarrita depending on the season and management of green pruning.$h[electronic resource] 260 $c2017 650 $afruits 650 $aPrunus Persica 653 $aCultural practice 700 1 $aPIROLA, K. 700 1 $aWAGNER JUNIOR, A. 700 1 $aRADAELLI, J. C. 700 1 $aANTUNES, L. E. C. 700 1 $aCITADIN, I. 773 $tBrazilian Journal of Agriculture$gv. 92, n. 3, p. 261-270, 2017.
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Embrapa Clima Temperado (CPACT) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
16/02/2017 |
Data da última atualização: |
30/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MUNK, M.; LADEIRA, L. O.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; RAPOSO, N. R. B.; SERAPIÁO, R. V.; QUINTAO, C. C. R.; SILVA, S. R.; SOARES, J. S.; JORIO, A.; BRANDAO, H. de M. |
Afiliação: |
Michele Munk, UFJF; Luiz O. Ladeira, UFMG; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Nádia R. B. Raposo, UFJF; Raquel V. Serapião, PESAGRO; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; Saulo R. Silva; Jaqueline S. Soares, UFOP; Ado Jorio, UFMG; HUMBERTO DE MELLO BRANDAO, CNPGL. |
Título: |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Nature, Scientific Reports, v. 6, article 33588, 2016. |
Idioma: |
Inglês |
Conteúdo: |
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos. |
Palavras-Chave: |
Gene delivery; Pellucid zone. |
Thesaurus NAL: |
in vitro fertilization. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155955/1/Cnpgl-2016-Nature-Efficient.pdf
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Marc: |
LEADER 01768naa a2200277 a 4500 001 2064560 005 2023-01-30 008 2016 bl uuuu u00u1 u #d 100 1 $aMUNK, M. 245 $aEfficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.$h[electronic resource] 260 $c2016 520 $aThe pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos. 650 $ain vitro fertilization 653 $aGene delivery 653 $aPellucid zone 700 1 $aLADEIRA, L. O. 700 1 $aCARVALHO, B. C. de 700 1 $aCAMARGO, L. S. de A. 700 1 $aRAPOSO, N. R. B. 700 1 $aSERAPIÁO, R. V. 700 1 $aQUINTAO, C. C. R. 700 1 $aSILVA, S. R. 700 1 $aSOARES, J. S. 700 1 $aJORIO, A. 700 1 $aBRANDAO, H. de M. 773 $tNature, Scientific Reports$gv. 6, article 33588, 2016.
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