Data/hora: 10/06/2024 07:49:24

Biblioteca(s): | Embrapa Soja.
Data corrente: | 03/12/1992
Data da última atualização: | 26/10/2004
Autoria: | CIAFARDINI, G.; BARBIERI, C.; GRAZIANI, L.
Título: | Nodulation activity of Rhizobium fredii USDA 201 transconjugant strains on several soybean cultivars.
Ano de publicação: | 1989
Fonte/Imprenta: | In: CONFERENCIA MUNDIAL DE INVESTIGACION EN SOJA, 4., 1989, Buenos Aires. Actas... Buenos Aires: AASOJA, 1989.
Volume: | t.1
Páginas: | p.521-526.
Idioma: | Inglês
Conteúdo: | The nodulation activity of several transconjugant strains derived from Rhizobium fredii USDA 201 was studied on 11 commercial soybean cultivars and on the Peking Chinese cultivar. The transconjugants were produced by complementing several Nod- Tn5 and Nod- Mu-dI lac Z mutant strains of R. fredii USDA 201 with a 23 kb DNA segment from Bradyrhizobium japonicum USDA 122 DES gene bank and a 20 kb segment from Rhizobium meliloti 1021 gene bank. The transconjugants restored with DNA from B. japonicum nodulated both the Peking Chinese cultivar and 82% of the tested U.S. commercial soybean, whereas those transconjugants restored with DNA from R. meliloti nodulated only with Peking soybean. The wild type R. fredii USDA 201 produced nodules on Peking cultivar and occasionally on 36% of the commercial cultivars. The acquisition and improvement of nodulation activity of R. fredii transconjugants active on commercial soybean cultivars took place following the tolerance acquisition towards several inhibitors of bacterial growth observed in extracts and root exudates from these plants, but not in those from Peking cultivars. Tolerance to growth inhibitors present in the roots was acquired only when the 23 kb segment fom B. japonicum USDA 122 DES gene bank was transferred into the mutants. Unlike R. fredii USDA 201 (fast-growth wild type), the B. japonicum USDA 122 DES showed tolerance towards both the compounds present in root extracts of Peking cultivars and the commercial cultivars.
Palavras-Chave: | Cultivar; Italia; Rhizobium fredii; Soybean; Variety.
Thesagro: | Bactéria; Bradyrhizobium Japonicum; Nodulação; Soja.
Thesaurus Nal: | Italy; nodulation.
Categoria do assunto: | --
Registro original: | Embrapa Soja (CNPSO)