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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
30/06/2010 |
Data da última atualização: |
18/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. |
Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP-JABOTICABAL; JORG PATRICK BURGSTALLER, UNIVERSTIY OF VETERINARY MEDICINE - VIENNA; FELIPE PERECIN, UNESP - JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP - JABOTICABAL; MARCOS ROBERTO CHIARATTI, USP - PIRASSUNUNGA; SIMONE CRISTINA MÉO, CPPSE; MATHIAS MULLER, UNIVERSITY OF VETERINARY MEDICINE - VIENNA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL - QUEBEC; FLÁVIO VIEIRA MEIRELLES, USP - PIRASSUNUNGA; RALF STEINBORN, UNIVERSITY OF VETERINARY MEDICINE - VIENNA. |
Título: |
Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010. |
DOI: |
https://doi.org/10.1095/biolreprod.109.080564 |
Idioma: |
Inglês |
Conteúdo: |
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. |
Palavras-Chave: |
Bovine; Developmental biology; Embryo; Gamete biology; Ooplasmic. |
Thesagro: |
DNA. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28805/1/PROCISCMN2010.00002.pdf
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Marc: |
LEADER 02370naa a2200313 a 4500 001 1856388 005 2022-11-18 008 2010 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1095/biolreprod.109.080564$2DOI 100 1 $aFERREIRA, C. R. 245 $aPronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.$h[electronic resource] 260 $c2010 520 $aOoplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. 650 $aDNA 653 $aBovine 653 $aDevelopmental biology 653 $aEmbryo 653 $aGamete biology 653 $aOoplasmic 700 1 $aBURGSTALLER, J. P. 700 1 $aPERECIN, F. 700 1 $aGARCIA, J. M. 700 1 $aCHIARATTI, M. R. 700 1 $aMÉO, S. C. 700 1 $aMULLER, M. 700 1 $aSMITH, L. C. 700 1 $aMEIRELLES, F. V. 700 1 $aSTEINBORN, R. 773 $tBiology of Reproduction$gv. 82, n. 03, p. 563-571, mar. 2010.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Unidades Centrais. |
Data corrente: |
12/11/2019 |
Data da última atualização: |
09/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
BERNARDI, A. C. de C.; PITRAT, T.; RABELLO, L. M.; PEZZOPANE, J. R. M.; BOSI, C.; MAZZUCO, G. G.; BETTIOL, G. M. |
Afiliação: |
ALBERTO CARLOS DE CAMPOS BERNARDI, CPPSE; Thomas Pitrat, Geocarta; LADISLAU MARCELINO RABELLO, CNPDIA; JOSE RICARDO MACEDO PEZZOPANE, CPPSE; Cristian Bosi, USP/ESALQ; Giulia Guillen Mazzuco, Secretaria Municipal de Turismo e Meio Ambiente de Itirapina; GIOVANA MARANHAO BETTIOL, CPAC. |
Título: |
Differences in soil electrical resistivity tomography due to soil water contents in an integrated agricultural system. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, v.54, e00774, p. 1-5, 2019. |
DOI: |
10.1590/S1678-3921.pab2019.v54.00774 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this work was to characterize the spatial variability of soil electrical resistivity due to different soil moisture contents, in an integrated agricultural system. Soil electrical resistivity (ER) was measured with the Automatic Resistivity Profiling (ARP) contact sensor in two dates, in 2016, in a 9.7-ha area with different soil moisture contents. The obtained maps indicated that ER allowed delimiting the regions within the study area and pointing out differences in the movement and accumulation of water in the soil horizons. Although there is a trend of reduction in ER values with increasing soil moisture, the spatial correlation structure of ER is similar. |
Palavras-Chave: |
ARP system; ILPF; Resistividade elétrica do solo; Sensor de solo; Sistema ARP; Soil sensor; Teor de água no solo. |
Thesagro: |
Solo; Umidade do Solo. |
Thesaurus NAL: |
Soil water content. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 01678naa a2200325 a 4500 001 2114214 005 2024-01-09 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1590/S1678-3921.pab2019.v54.00774$2DOI 100 1 $aBERNARDI, A. C. de C. 245 $aDifferences in soil electrical resistivity tomography due to soil water contents in an integrated agricultural system.$h[electronic resource] 260 $c2019 520 $aThe objective of this work was to characterize the spatial variability of soil electrical resistivity due to different soil moisture contents, in an integrated agricultural system. Soil electrical resistivity (ER) was measured with the Automatic Resistivity Profiling (ARP) contact sensor in two dates, in 2016, in a 9.7-ha area with different soil moisture contents. The obtained maps indicated that ER allowed delimiting the regions within the study area and pointing out differences in the movement and accumulation of water in the soil horizons. Although there is a trend of reduction in ER values with increasing soil moisture, the spatial correlation structure of ER is similar. 650 $aSoil water content 650 $aSolo 650 $aUmidade do Solo 653 $aARP system 653 $aILPF 653 $aResistividade elétrica do solo 653 $aSensor de solo 653 $aSistema ARP 653 $aSoil sensor 653 $aTeor de água no solo 700 1 $aPITRAT, T. 700 1 $aRABELLO, L. M. 700 1 $aPEZZOPANE, J. R. M. 700 1 $aBOSI, C. 700 1 $aMAZZUCO, G. G. 700 1 $aBETTIOL, G. M. 773 $tPesquisa Agropecuária Brasileira, Brasília$gv.54, e00774, p. 1-5, 2019.
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