Data/hora: 04/06/2024 04:44:07

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	15/08/2016	
Data da última atualização: 	02/12/2016	
Autoria: 	CHAVES, J. da S.; BARAÚNA, A. C.; MOSQUEIRA, C. A.; GIANLUPPI, V.; ZILLI, J. E.; SILVA, K. da.	
Afiliação: 	JOSIMAR DA SILVA CHAVES, UFRR; ALEXANDRE CARDOSO BARAÚNA, UFRRJ; CÁTIA APARECIDA MOSQUEIRA, UFRR; VICENTE GIANLUPPI, CPAF-Roraima; JERRI EDSON ZILLI, CNPAB; KRISLE DA SILVA, CPAF-Roraima.	
Título: 	Stylosanthes spp. from Amazon savanna harbour diverse and potentially effective rhizobia.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Applied Soil Ecology, v. 108, p. 54-61, 2016	
Idioma: 	Inglês	
Conteúdo: 	The Stylosanthes genus stands out among the forage legume species well-adapted to the soil and climatic conditions of tropical regions. This legume establishes a symbiotic relationship with nitrogen-fixing bacteria, which can be used to improve the quality of pastures. However, there are few studies about the diversity and symbiotic efficiency of nitrogen-fixing bacteria in Stylosanthes plants. Therefore, the objective was to isolate, characterize and evaluate the symbiotic efficiency of nitrogen-fixing bacteria in symbiosis with Stylosanthes in savanna areas in Roraima, Brazil. Plants with nodules were collected at 6 sites, including cultivated and native areas of the savanna. Shoots of Stylosanthes with flowers were collected to prepare exsiccatae for species identification. The nodules were surface-sterilized with 5% sodium hypochlorite after the roots were washed, and isolation and morphological characterization were performed in 79 culture medium. The isolates were selected according to their morphology, the representativeness of each collection site and the associated Stylosanthes species. Representative isolates were tested for nodulation in cowpea plants, and the isolates able to nodulate the gene 16S rRNA was sequenced. The symbiotic efficiency was evaluated in Stylosanthes capitata cv. Lavradeiro in greenhouse conditions for 65 days. A total of 504 nodules were collected, from which 258 pure isolates were obtained from 5 species of Stylosanthes (S. capitata,S. scabra, S. gracilis, S. humilis and S. angustifolia). The isolates showed significant phenotypic diversity, and 89 isolates were selected. Thirty-one isolates were confirmed to be nodulating bacteria in cowpea plants. These isolates were identified as a-Proteobacteria, with Bradyrhizobium, rhizobium and Mesorhizobium genera, and b-Proteobacteria, with Burkholderia and Herbaspirillum genera. When evaluated in S. capitata, treatment with two Bradyrhizobium isolates, ERR 1178 and ERR 942, resulted in significant production of plant biomass production and N accumulation. These results indicated that a large diversity of a-Proteobacteria and b-Proteobacteria are able to nodulate Stylosanthes spp. and some isolates have potential for testing under
field conditions.	
Palavras-Chave: 	16S rRNA; Biological nitrogen fixation; Eficiência simbiótica; FBN; Forragem tropical; Symbiotic efficiency; Tropical forage.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (40302-1) - UPC - SP - DD - 2016.00131 -  - 2016.00131 - DIS