Data/hora: 06/06/2024 17:07:45

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	16/11/2017	
Data da última atualização: 	23/11/2017	
Autoria: 	RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; GUERRA-PERAZA, O.; TASSI, A. D.; KITAJIMA, E. W.; HARAKAVA, R.; SALAROLI, R. B.; ASTUA, J. de F.	
Afiliação: 	PEDRO LUIS RAMOS-GONZÁLEZ; CAMILA CHABI-JESUS; ORLENE GUERRA-PERAZA; ALINE DANIELE TASSI; ELLIOT WATANABE KITAJIMA; RICARDO HARAKAVA; RENATO BARBOSA SALAROLI; JULIANA DE FREITAS ASTUA, CNPMF.	
Título: 	Citrus leprosis virus N: a new dichorhavirus causing citrus leprosis disease.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Phytopathology, v.107, n.8, August 2017.	
ISSN: 	0031-949X	
DOI: 	10.1094/PHYTO-02-17-0042-R	
Idioma: 	Inglês	
Conteúdo: 	Citrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis plants were anatomically recognized as Brevipalpus phoenicis sensu stricto. Molecular and biological features indicate that the identified viruses belong to a new species of CL-associated dichorhavirus, which we propose to call Citrus leprosis N dichorhavirus. Our results, while emphasizing the increasing diversity of viruses causing CL disease, lead to a reevaluation of the nomenclature of those viruses assigned to the genus Dichorhavirus. In this regard, a comprehensive discussion is presented.	
Palavras-Chave: 	Plant disease.	
Thesagro: 	Doença de planta; Fruta cítrica.	
Thesaurus Nal: 	Citrus.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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