Data/hora: 01/06/2024 06:32:56

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 29/07/2015
Data da última atualização: | 18/05/2023
Autoria: | RAMOS-GONZALEZ, P. L.; SARUBBI-ORUE, H.; GONZALES-SEGNANA, L.; CHABI-JESUS, C.; ASTUA, J. de F.; KITAJIMA, E. W.
Afiliação: | PEDRO LUIS RAMOS-GONZALEZ, IAC; HUMBERTO SARUBBI-ORUE, Universidad Nacional de Asuncion; LUIS GONZALES-SEGNANA, Universidad Nacional de Asuncion; CAMILA CHABI-JESUS, IAC; JULIANA DE FREITAS ASTUA, CNPMF; ELIOT WATANABE KITAJIMA, ESALQ.
Título: | Orchid Fleck Virus Infecting Orchids in Paraguay: First Report and Use of Degenerate Primers for its Detection.
Ano de publicação: | 2015
Fonte/Imprenta: | Journal of Phytopathology, 2015.
Páginas: | 6p.
Série: | 1439-0434
Idioma: | Inglês
Conteúdo: | The presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples.
Palavras-Chave: | Citrus leprosis virus.
Thesagro: | Doença de planta; Orchidaceae; Orquídea.
Categoria do assunto: | O Insetos e Entomologia
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (30731-1) - UPC - AP - DD - Publicação digital -  -  - DIS