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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
22/03/2016 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PÉREZ-MONTAÑO, F.; CERRO, P. del; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; CUBO, M. T.; HUNGRIA, M.; MEGÍAS, M.; OLLERO, F. J. |
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Afiliação: |
Francisco Péres-Montaño, Universidade de Sevilla; Pablo del Cerro, Universidade de Sevilia; Irene Jiménez-Guerreiro, Universidade de Sevilla; Francisco Javier López-Baena, Universidade de Sevilla; maria Teresa Cubo, Universidade de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; Manuel Megías, Universidade de Sevilla; Francisco Javier Ollero, Uiversidade de Sevilla. |
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Título: |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
BMC Genomics, v. 17, n. 1, p. 198, Mar. 2016. |
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ISSN: |
1471-2164 |
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DOI: |
10.1186/s12864-016-2543-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. MenosRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the e... Mostrar Tudo |
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Palavras-Chave: |
Lipochitooligosaccharides; Nod factors; Nodutlation; RNA-seq. |
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Thesagro: |
Nodulação; Rhizobium. |
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Thesaurus Nal: |
Apigenin; Rhizobium tropici; Salt stress. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142584/1/RNA-seq-analysis-of-the-rhizobium...pdf
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Marc: |
LEADER 02939naa a2200337 a 4500
001 2041600
005 2017-06-19
008 2016 bl uuuu u00u1 u #d
022 $a1471-2164
024 7 $a10.1186/s12864-016-2543-3$2DOI
100 1 $aPÉREZ-MONTAÑO, F.
245 $aRNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt.$h[electronic resource]
260 $c2016
520 $aRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions.
650 $aApigenin
650 $aRhizobium tropici
650 $aSalt stress
650 $aNodulação
650 $aRhizobium
653 $aLipochitooligosaccharides
653 $aNod factors
653 $aNodutlation
653 $aRNA-seq
700 1 $aCERRO, P. del
700 1 $aJIMÉNEZ-GUERRERO, I.
700 1 $aLÓPEZ-BAENA, F. J.
700 1 $aCUBO, M. T.
700 1 $aHUNGRIA, M.
700 1 $aMEGÍAS, M.
700 1 $aOLLERO, F. J.
773 $tBMC Genomics$gv. 17, n. 1, p. 198, Mar. 2016.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 127 | |
| 6. |  | ROSA, C. da; SINIGAGLIA, L.; PRETO, G. A. S.; GROHS, D. S. Formation of callus on rootstock cuttings with different substrates during the forcing. In: WORLD VINE AND WINE CONGRESS, 39.; GENERAL ASSEMBLY OF THE OIV, 14., 2016, Bento Gonçalves. Vitiviniculture: technological advances to market challenges: abstracts. Bento Gonçalves: International Organisation of Vine and Wine, n. 2016-1010, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | ROSA, C. L. S.; FREITAS, D. G. C.; ROCHA, M. C.; SOARES, A. G.; ABBOUD, A. Qualidade de polpas concentradas de tomates 'heirloom' produzidos sob manejo orgânico. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 9., 2011, Campinas. Ciência de alimentos e qualidade de vida: saúde, meio ambiente e sustentabilidade: resumos. Campinas: UNICAMP, FEA, 2011. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 14. | | FREITAS, D.; ROSA, C.; ROCHA, R.; SILVA, A.; SOARES, A.; ABBOUD, A. Sensory profile of tomato concentrated from 'Heirloom' genotypes produced under organic management. In: PANGBORN SENSORY SCIENCE SYMPOSIUM, 9., 2011, Toronto. Conference abstracts. Oxford: Elsevier, 2011. 1 CD-ROM. Ref. P1.1.07.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 16. | | REIS, E. F.; LIMA NETO, V. da C.; GODOY, C. V.; CASTANHO, H. E.; VICENTE, N. G.; ROSA, C. T. Avaliação do controle da ferrugem da soja (Phakopsora spp.) utilizando triazol e mistura de triazol e estrobilurina. Fitopatologia Brasileira, Brasília, v. 29, p. S180, ago. 2004. Suplemento. Resumo apresentado no XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, agosto, 2004.| Biblioteca(s): Embrapa Soja. |
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| 17. | | REIS, E. F dos; LIMA NETO, V. da C.; GODOY, C. V.; CASTANHO, H. E.; VICENTE, N. G.; ROSA, C. Controle químico da ferrugem da soja (Phakopsora spp.). Fitopatologia Brasileira, Brasília, v. 29, p. S177-S178, ago. 2004. Suplemento. Resumo apresentado no XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, agosto, 2004.| Biblioteca(s): Embrapa Soja. |
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| 18. | | FARIAS, M. de O.; ROSA, C. M. da; DUPONT, P. B.; MATIESKI, T.; CASTILHOS, R. M. V.; PILLON, C. N. Composição elementar de ácidos húmicos isolados da camada superficial de um planossolo háplico, submetido a diferentes sisitemas de manejo. In: ENCONTRO BRASILEIRO DE SUBSTÂNCIAS HÚMICAS, 8., 2009. Pelotas. Resumos... Pelotas: Embrapa Clima Temperado, 2009. p. 49.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 19. | | ZSCHORMACK, T.; FERNANDES, F. F.; PILLON, C. N.; ROSA, C. M. da; TOMAZI, M.; CONCEIÇÃO, P. C.; BAYER, C. Carbono orgânico total e frações da matéria orgânica em dois solos sob campo natural no sul do Rio Grande do Sul. In: ENCONTRO BRASILEIRO DE SUBSTÂNCIAS HÚMICAS, 7., 2007, Florianopólis, Resumos... Florianopólis: UFSC, 2007. p. 32| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 127 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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