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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
22/07/2015 |
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Data da última atualização: |
22/07/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SCHULZ, K.; HUNGER, S.; BROWN, G. G.; TSAI, S. M.; CERRI, C. C.; CONRAD, R.; DRAKE, H. L. |
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Afiliação: |
Kristin Schulz, University of Bayreuth; Sindy Hunger, University of Bayreuth; GEORGE GARDNER BROWN, CNPF; Siu M. Tsai, USP; Carlos C. Cerri, USP; Ralf Conrad, Max-Planck-Institute for Terrestrial Microbiology; Harold L. Drake, University of Bayreuth. |
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Título: |
Methanogenic food web in the gut contents of methane-emitting earthworm Eudrilus eugeniae from Brazil. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
The ISME Journal, v. 9, n. 8, p. 1778-1792, Aug. 2015. |
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DOI: |
10.1038/ismej.2014.262 |
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Idioma: |
Inglês |
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Conteúdo: |
The anoxic saccharide-rich conditions of the earthworm gut provide an ideal transient habitat for ingested microbes capable of anaerobiosis. It was recently discovered that the earthworm Eudrilus eugeniae from Brazil can emit methane (CH4) and that ingested methanogens might be associated with this emission. The objective of this study was to resolve trophic interactions of bacteria and methanogens in the methanogenic food web in the gut contents of E. eugeniae. RNA-based stable isotope probing of bacterial 16S rRNA as well as mcrA and mrtA (the alpha subunit of methyl-CoM reductase and its isoenzyme, respectively) of methanogens was performed with [(13)C]-glucose as a model saccharide in the gut contents. Concomitant fermentations were augmented by the rapid consumption of glucose, yielding numerous products, including molecular hydrogen (H2), carbon dioxide (CO2), formate, acetate, ethanol, lactate, succinate and propionate. Aeromonadaceae-affiliated facultative aerobes, and obligate anaerobes affiliated to Lachnospiraceae, Veillonellaceae and Ruminococcaceae were associated with the diverse fermentations. Methanogenesis was ongoing during incubations, and (13)C-labeling of CH4 verified that supplemental [(13)C]-glucose derived carbon was dissimilated to CH4. Hydrogenotrophic methanogens affiliated with Methanobacteriaceae and Methanoregulaceae were linked to methanogenesis, and acetogens related to Peptostreptoccocaceae were likewise found to be participants in the methanogenic food web. H2 rather than acetate stimulated methanogenesis in the methanogenic gut content enrichments, and acetogens appeared to dissimilate supplemental H2 to acetate in methanogenic enrichments. These findings provide insight on the processes and associated taxa potentially linked to methanogenesis and the turnover of organic carbon in the alimentary canal of methane-emitting E. eugeniae. MenosThe anoxic saccharide-rich conditions of the earthworm gut provide an ideal transient habitat for ingested microbes capable of anaerobiosis. It was recently discovered that the earthworm Eudrilus eugeniae from Brazil can emit methane (CH4) and that ingested methanogens might be associated with this emission. The objective of this study was to resolve trophic interactions of bacteria and methanogens in the methanogenic food web in the gut contents of E. eugeniae. RNA-based stable isotope probing of bacterial 16S rRNA as well as mcrA and mrtA (the alpha subunit of methyl-CoM reductase and its isoenzyme, respectively) of methanogens was performed with [(13)C]-glucose as a model saccharide in the gut contents. Concomitant fermentations were augmented by the rapid consumption of glucose, yielding numerous products, including molecular hydrogen (H2), carbon dioxide (CO2), formate, acetate, ethanol, lactate, succinate and propionate. Aeromonadaceae-affiliated facultative aerobes, and obligate anaerobes affiliated to Lachnospiraceae, Veillonellaceae and Ruminococcaceae were associated with the diverse fermentations. Methanogenesis was ongoing during incubations, and (13)C-labeling of CH4 verified that supplemental [(13)C]-glucose derived carbon was dissimilated to CH4. Hydrogenotrophic methanogens affiliated with Methanobacteriaceae and Methanoregulaceae were linked to methanogenesis, and acetogens related to Peptostreptoccocaceae were likewise found to be participants in the methan... Mostrar Tudo |
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Palavras-Chave: |
Gás de efeito estufa; Microdiversidade microbiana. |
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Thesagro: |
Bactéria; Metano; Minhoca. |
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Thesaurus Nal: |
Eudrilus eugeniae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02702naa a2200277 a 4500
001 2020241
005 2015-07-22
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1038/ismej.2014.262$2DOI
100 1 $aSCHULZ, K.
245 $aMethanogenic food web in the gut contents of methane-emitting earthworm Eudrilus eugeniae from Brazil.$h[electronic resource]
260 $c2015
520 $aThe anoxic saccharide-rich conditions of the earthworm gut provide an ideal transient habitat for ingested microbes capable of anaerobiosis. It was recently discovered that the earthworm Eudrilus eugeniae from Brazil can emit methane (CH4) and that ingested methanogens might be associated with this emission. The objective of this study was to resolve trophic interactions of bacteria and methanogens in the methanogenic food web in the gut contents of E. eugeniae. RNA-based stable isotope probing of bacterial 16S rRNA as well as mcrA and mrtA (the alpha subunit of methyl-CoM reductase and its isoenzyme, respectively) of methanogens was performed with [(13)C]-glucose as a model saccharide in the gut contents. Concomitant fermentations were augmented by the rapid consumption of glucose, yielding numerous products, including molecular hydrogen (H2), carbon dioxide (CO2), formate, acetate, ethanol, lactate, succinate and propionate. Aeromonadaceae-affiliated facultative aerobes, and obligate anaerobes affiliated to Lachnospiraceae, Veillonellaceae and Ruminococcaceae were associated with the diverse fermentations. Methanogenesis was ongoing during incubations, and (13)C-labeling of CH4 verified that supplemental [(13)C]-glucose derived carbon was dissimilated to CH4. Hydrogenotrophic methanogens affiliated with Methanobacteriaceae and Methanoregulaceae were linked to methanogenesis, and acetogens related to Peptostreptoccocaceae were likewise found to be participants in the methanogenic food web. H2 rather than acetate stimulated methanogenesis in the methanogenic gut content enrichments, and acetogens appeared to dissimilate supplemental H2 to acetate in methanogenic enrichments. These findings provide insight on the processes and associated taxa potentially linked to methanogenesis and the turnover of organic carbon in the alimentary canal of methane-emitting E. eugeniae.
650 $aEudrilus eugeniae
650 $aBactéria
650 $aMetano
650 $aMinhoca
653 $aGás de efeito estufa
653 $aMicrodiversidade microbiana
700 1 $aHUNGER, S.
700 1 $aBROWN, G. G.
700 1 $aTSAI, S. M.
700 1 $aCERRI, C. C.
700 1 $aCONRAD, R.
700 1 $aDRAKE, H. L.
773 $tThe ISME Journal$gv. 9, n. 8, p. 1778-1792, Aug. 2015.
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Embrapa Florestas (CNPF) |
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| 2. |  | CARVALHO, M. da C, S.; CERRI, C. C. Distribuição de raízes e atributos químicos de um neossolo quartzarênico sob pastagem degradada submetida a práticas de recuperação. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28., REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12., SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10., REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina, PR. Desafios para o uso do solo com eficiência e qualidade ambiental: anais... Londrina: SBCS: Embrapa Soja, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Algodão. |
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| 7. | | CARVALHO, J. L. N.; AVANZI, J. C.; CERRI, C. E. P.; CERRI, C. C. Adequação dos sistemas de produção rumo à sustentabilidade ambiental. In: FALEIRO, F. G.; FARIAS NETO, A. L. de (Ed.). Savanas: desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais. Planaltina, DF: Embrapa Cerrados, 2008. cap. 19, p. 673-692.| Biblioteca(s): Embrapa Cerrados. |
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| 14. | | FERNANDES, S. A. P.; BETTIOL, W.; CERRI, C. C.; CAMARGO, Plínio. Sewage sludge effects on gas fluxes at the soil-atmosphere interface, on soil d13C and on total soil carbon and nitrogen. Geoderma, Amsterdam, v. 125, n. 1-2, p. 49-57, 2005.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 16. | | CAMPOS, D. C. de; ABBAS, M. Z. M.; LUCA, E. F. de; MARTIN-NETO, L.; CERRI, C. C.; ESCHENBRENNER, V. Constituição de um horizonte de "húmus" após quatro anos de manejo sem queima da can-de-açúcar. In: ENCONTRO BRASILEIRO DE SUBSTÂNCIAS HÚMICAS-IHSS, 4., 2001, Viçosa. IHSS 2001: resumos de palestras e trabalhos apresentados. Viçosa: UFV-Departamento de Solos: Grupo Brasileiro da IHSS, 2001. p. 105-107.| Biblioteca(s): Embrapa Instrumentação. |
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| 17. | | NORONHA, N. C.; ANDRADE, C. A. de; CERRI, C. C.; PICCOLO, M. de C.; COSTA, J. J. F. Disponibilidade do ferro em sistemas de recuperação de pastagens. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011. 1 CD-ROM. 4 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 18. | | COSTA JUNIOR, C.; PICCOLO, M. de C.; SIQUEIRA NETO, M.; BERNOUX, M.; CERRI, C. C.; SCOPEL, E. Efeito de sistemas de manejo na estabilidade de agregados e nos teores de carbono em um latossolo vermelho em Rio Verde, GO. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira: anais. Porto Alegre: SBCS, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 30 | |
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