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| 1. |  | JULIEN, M. H.; HILL, M. P.; CENTER, T. D.; JIANQING, D. (ed.). Biological and integrated control of water hyacinth, Eichhornia crassipes: proceedings of the second meeting of the Global Working Group for the Biological and Integrated Control of Water Hyacinth, Beijing, China, 9-12 Oct. 2000. Canberra: ACIAR, 2001. 152 p. (ACIAR proceedings, n. 102).| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
12/01/2017 |
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Data da última atualização: |
02/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ORLANDO, E. A.; ROQUE, A. G. C.; LOSEKANN, M. E.; SIMIONATO, A. V. C. |
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Afiliação: |
EDUARDO ADILSON ORLANDO, ITAL; ALINE GABRIELA COSTA ROQUE, ITAL; MARCOS ELISEU LOSEKANN, CNPMA; ANA VALERIA COLNAGHI SIMIONATO, IQ-UNICAMP. |
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Título: |
UPLC-MS/MS determination of florfenicol and florfebicol amine antimicrobial residues in tilapia muscle. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Chromatography B, v. 1035, p. 8-15, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Despite the benefits to fish farmers, the use of antimicrobials in aquaculture has concerned consumers and competent authorities. The indiscriminate use of such substances promotes the emergence of resistant microorganisms, decreases the effectiveness of treatments, and causes possible toxic effects in humans. In Brazil, florfenicol is the only antimicrobial registered for use in aquaculture and is often used in tilapia in cage creation. Thus, this study aimed to develop a method for determination of florfenicol residues and its metabolite florfenicol amine in tilapia fillet by UPLC-MS/MS. Analytes were extracted with ethyl acetate, followed by liquid-liquid partition clean-up with hexane and SPE. The sorbents C18, phenyl and HLB-Oasis were evaluated by SPE. Phenyl sorbent showed the best results, and the extraction conditions were optimized in the sample matrix with fractional factorial design 24-1. The analytes were separated on a C18 chromatographic column (50 × 2.1 mm × 1.7 microm) using water (A) and acetonitrile (B) as mobile phase at a flow rate of 0.3 mL min-1 with a linear gradient (in% B): 0-2.0 min: 20%; 2.0-2.5 min: increase to 90%; 2.5-3.5 min: 90%; 3.0-3.5 min: decrease to 20%; 4.0-5.0 min: 20%. The analytes were monitored in a MS/MS triple quadrupole system by MRM mode with transitions at m/z 356.1 > 336.1 (florfenicol) and m/z 248.1 > 130.1 (florfenicol amine). The optimized method was validated obtaining LOQ values of 3 and 25 ng g-1 for florfenicol and florfenicol amine, respectively, precision between 20 and 36%, absolute extraction efficiency between 38 and 80%, and adequate linearity. The method was applied to samples intended for human consumption, and within the 15 evaluated samples, only one showed florfenicol residue at 30 ng g-1, which is below the maximum residue limit established in Brazil. MenosAbstract: Despite the benefits to fish farmers, the use of antimicrobials in aquaculture has concerned consumers and competent authorities. The indiscriminate use of such substances promotes the emergence of resistant microorganisms, decreases the effectiveness of treatments, and causes possible toxic effects in humans. In Brazil, florfenicol is the only antimicrobial registered for use in aquaculture and is often used in tilapia in cage creation. Thus, this study aimed to develop a method for determination of florfenicol residues and its metabolite florfenicol amine in tilapia fillet by UPLC-MS/MS. Analytes were extracted with ethyl acetate, followed by liquid-liquid partition clean-up with hexane and SPE. The sorbents C18, phenyl and HLB-Oasis were evaluated by SPE. Phenyl sorbent showed the best results, and the extraction conditions were optimized in the sample matrix with fractional factorial design 24-1. The analytes were separated on a C18 chromatographic column (50 × 2.1 mm × 1.7 microm) using water (A) and acetonitrile (B) as mobile phase at a flow rate of 0.3 mL min-1 with a linear gradient (in% B): 0-2.0 min: 20%; 2.0-2.5 min: increase to 90%; 2.5-3.5 min: 90%; 3.0-3.5 min: decrease to 20%; 4.0-5.0 min: 20%. The analytes were monitored in a MS/MS triple quadrupole system by MRM mode with transitions at m/z 356.1 > 336.1 (florfenicol) and m/z 248.1 > 130.1 (florfenicol amine). The optimized method was validated obtaining LOQ values of 3 and 25 ng g-1 for florfenico... Mostrar Tudo |
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Palavras-Chave: |
Florfenicol amine; Residue; SPE. |
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Thesagro: |
Análise química; Antibiótico; Cromatografia; Florfenicol; Oreochromis Niloticus; Resíduo; Tilápia. |
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Thesaurus NAL: |
Anti-infective agents; Drug residues; fish. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02744naa a2200313 a 4500
001 2060653
005 2017-03-02
008 2016 bl uuuu u00u1 u #d
100 1 $aORLANDO, E. A.
245 $aUPLC-MS/MS determination of florfenicol and florfebicol amine antimicrobial residues in tilapia muscle.$h[electronic resource]
260 $c2016
520 $aAbstract: Despite the benefits to fish farmers, the use of antimicrobials in aquaculture has concerned consumers and competent authorities. The indiscriminate use of such substances promotes the emergence of resistant microorganisms, decreases the effectiveness of treatments, and causes possible toxic effects in humans. In Brazil, florfenicol is the only antimicrobial registered for use in aquaculture and is often used in tilapia in cage creation. Thus, this study aimed to develop a method for determination of florfenicol residues and its metabolite florfenicol amine in tilapia fillet by UPLC-MS/MS. Analytes were extracted with ethyl acetate, followed by liquid-liquid partition clean-up with hexane and SPE. The sorbents C18, phenyl and HLB-Oasis were evaluated by SPE. Phenyl sorbent showed the best results, and the extraction conditions were optimized in the sample matrix with fractional factorial design 24-1. The analytes were separated on a C18 chromatographic column (50 × 2.1 mm × 1.7 microm) using water (A) and acetonitrile (B) as mobile phase at a flow rate of 0.3 mL min-1 with a linear gradient (in% B): 0-2.0 min: 20%; 2.0-2.5 min: increase to 90%; 2.5-3.5 min: 90%; 3.0-3.5 min: decrease to 20%; 4.0-5.0 min: 20%. The analytes were monitored in a MS/MS triple quadrupole system by MRM mode with transitions at m/z 356.1 > 336.1 (florfenicol) and m/z 248.1 > 130.1 (florfenicol amine). The optimized method was validated obtaining LOQ values of 3 and 25 ng g-1 for florfenicol and florfenicol amine, respectively, precision between 20 and 36%, absolute extraction efficiency between 38 and 80%, and adequate linearity. The method was applied to samples intended for human consumption, and within the 15 evaluated samples, only one showed florfenicol residue at 30 ng g-1, which is below the maximum residue limit established in Brazil.
650 $aAnti-infective agents
650 $aDrug residues
650 $afish
650 $aAnálise química
650 $aAntibiótico
650 $aCromatografia
650 $aFlorfenicol
650 $aOreochromis Niloticus
650 $aResíduo
650 $aTilápia
653 $aFlorfenicol amine
653 $aResidue
653 $aSPE
700 1 $aROQUE, A. G. C.
700 1 $aLOSEKANN, M. E.
700 1 $aSIMIONATO, A. V. C.
773 $tJournal of Chromatography B$gv. 1035, p. 8-15, 2016.
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