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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Meio-Norte; Embrapa Semiárido. |
Data corrente: |
25/10/1995 |
Data da última atualização: |
29/07/2009 |
Autoria: |
ARAÚJO FILHO, J. A. de; TORRES, S. M. de; GADELHA, J. A.; MACIEL, D. F.; CATUNDA, A. G. |
Título: |
Estudos de pastagem nativa do Ceará. |
Ano de publicação: |
1982 |
Fonte/Imprenta: |
Fortaleza: BNB, 1982. |
Páginas: |
75 p. |
Série: |
(BNB. Estudos Econômicos e Sociais, 13). |
Idioma: |
Português |
Conteúdo: |
Estudos de pastagem nativa do Ceará; Flutuações mensais na produtividade e valor nutritivo de dois sítios ecológicos do sertao cearense; Introdução de gramíneas forrageiras em terra seca. |
Palavras-Chave: |
Anthephora pubescens; Brasil; Capim-piranha; Capim-touceira; Ceara; Eragrostis atherstonei; Eragrostis superba; Estrato arbóreo; Feed crops. |
Thesagro: |
Caatinga; Capim Buffel; Capim Urochloa; Forragem; Gramínea forrageira; Panicum Maximum; Pastagem; Pastagem nativa; Planta Forrageira; Urochloa Mosambicensis; Vegetação Nativa. |
Thesaurus Nal: |
Brazil; Grasses; Grazing lands; Pastures; Semiarid zones. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 01424nam a2200481 a 4500 001 1064045 005 2009-07-29 008 1982 bl uuuu 00u1 u #d 100 1 $aARAÚJO FILHO, J. A. de 245 $aEstudos de pastagem nativa do Ceará. 260 $aFortaleza: BNB$c1982 300 $a75 p. 490 $a(BNB. Estudos Econômicos e Sociais, 13). 520 $aEstudos de pastagem nativa do Ceará; Flutuações mensais na produtividade e valor nutritivo de dois sítios ecológicos do sertao cearense; Introdução de gramíneas forrageiras em terra seca. 650 $aBrazil 650 $aGrasses 650 $aGrazing lands 650 $aPastures 650 $aSemiarid zones 650 $aCaatinga 650 $aCapim Buffel 650 $aCapim Urochloa 650 $aForragem 650 $aGramínea forrageira 650 $aPanicum Maximum 650 $aPastagem 650 $aPastagem nativa 650 $aPlanta Forrageira 650 $aUrochloa Mosambicensis 650 $aVegetação Nativa 653 $aAnthephora pubescens 653 $aBrasil 653 $aCapim-piranha 653 $aCapim-touceira 653 $aCeara 653 $aEragrostis atherstonei 653 $aEragrostis superba 653 $aEstrato arbóreo 653 $aFeed crops 700 1 $aTORRES, S. M. de 700 1 $aGADELHA, J. A. 700 1 $aMACIEL, D. F. 700 1 $aCATUNDA, A. G.
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Embrapa Meio-Norte (CPAMN) |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
09/01/2019 |
Data da última atualização: |
12/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
VASCONCELOS, M. J. V. de; ANTUNES, M. S.; OLIVEIRA, M. F. de; LOPES, M. A.; FIGUEIREDO, J. E. F. |
Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; MAURICIO SCHUSTERSCHITZ ANTUNES, Colorado State University; MAURILIO FERNANDES DE OLIVEIRA, CNPMS; MAURICIO ANTONIO LOPES, DE/PR; JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
Título: |
Callus induction and plant regeneration from immature embryos culture of tropical maize. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 17, n. 3, p. 359-368, 2018. |
Idioma: |
Inglês |
Conteúdo: |
The development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. MenosThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that ... Mostrar Tudo |
Palavras-Chave: |
Calos tipo I e tipo II; Embriogênese somática. |
Thesagro: |
Cultura de Tecido; Embrião Vegetal; Zea Mays. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190064/1/Callus-induction.pdf
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Marc: |
LEADER 02500naa a2200229 a 4500 001 2103395 005 2019-02-12 008 2018 bl uuuu u00u1 u #d 100 1 $aVASCONCELOS, M. J. V. de 245 $aCallus induction and plant regeneration from immature embryos culture of tropical maize.$h[electronic resource] 260 $c2018 520 $aThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. 650 $aCultura de Tecido 650 $aEmbrião Vegetal 650 $aZea Mays 653 $aCalos tipo I e tipo II 653 $aEmbriogênese somática 700 1 $aANTUNES, M. S. 700 1 $aOLIVEIRA, M. F. de 700 1 $aLOPES, M. A. 700 1 $aFIGUEIREDO, J. E. F. 773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 17, n. 3, p. 359-368, 2018.
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