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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/04/1994 |
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Data da última atualização: |
25/04/1994 |
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Autoria: |
SORRIA, R. A.; GOMEZ, A.; CATANO, M. L.; HERRERA, P. V.; CALDERON, A.; MAYER, J. E.; ROCA, W. M. |
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Afiliação: |
CIAT, Cali, Colombia. |
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Título: |
Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Cali, Colombia: CIAT, 1993 |
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Páginas: |
216-221p |
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Série: |
CIAT,Working Document, 123 |
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Idioma: |
Inglês |
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Notas: |
International scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedinjs... Cali, Colombia: CIAT, 1993. |
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Conteúdo: |
Our strategy for Agrobacterium-mediated cassava transformation involves the utilization of cotyledonary leaves from somatic embryos and infection with both wild and disarmed strains. Virulence tests for wild Agrobacterium strains as well as regeneration protocols have been developed; the plasmid used in all the experiments was the pGV 1040 containing the uid A, the bar and the npt II genes (provided by Plant Genetic Systems, Gent). Sensitivity levels of cassava tissues to the commercial herbicide Basta, used as selection agent, were determined. Minimum lethal doses for the herbicide were 1 mg/l for immature leaves and 18mg/l for somatic embryos. Results with kanamyvin as selection agent were inconsistent. Only somatic embryos showed endogenous uid A gene exp pression; endogenous activity was reduced by 70-80% using the kosugi protocol (1990), which includes methanol. Expression of opine synthesis genes of a wild Agobacterium strain, isolated from cassava tumors, was obtained after transformation of cotyledonary leaves of somatic embryos with the wild strain. Transformation experiments are now under way using the wild Agrobacterium strain carrying the pGV 1040 plasmid. A biolistic device was used to bombard early stage somatic embryos using the pGV 1040 construct harboring spots per 0,5 cm2 of embryogenic tissue was obtained. GUS expression in proliferating somatic embryos has been observed after one and two months from bombardment. |
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Palavras-Chave: |
Congresso; Tecidos. |
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Thesagro: |
Embriogénese. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02290naa a2200265 a 4500
001 1644380
005 1994-04-25
008 1993 bl uuuu u00u1 u #d
100 1 $aSORRIA, R. A.
245 $aTowards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation.
260 $c1993
300 $a216-221p
490 $aCIAT,Working Document, 123
500 $aInternational scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedinjs... Cali, Colombia: CIAT, 1993.
520 $aOur strategy for Agrobacterium-mediated cassava transformation involves the utilization of cotyledonary leaves from somatic embryos and infection with both wild and disarmed strains. Virulence tests for wild Agrobacterium strains as well as regeneration protocols have been developed; the plasmid used in all the experiments was the pGV 1040 containing the uid A, the bar and the npt II genes (provided by Plant Genetic Systems, Gent). Sensitivity levels of cassava tissues to the commercial herbicide Basta, used as selection agent, were determined. Minimum lethal doses for the herbicide were 1 mg/l for immature leaves and 18mg/l for somatic embryos. Results with kanamyvin as selection agent were inconsistent. Only somatic embryos showed endogenous uid A gene exp pression; endogenous activity was reduced by 70-80% using the kosugi protocol (1990), which includes methanol. Expression of opine synthesis genes of a wild Agobacterium strain, isolated from cassava tumors, was obtained after transformation of cotyledonary leaves of somatic embryos with the wild strain. Transformation experiments are now under way using the wild Agrobacterium strain carrying the pGV 1040 plasmid. A biolistic device was used to bombard early stage somatic embryos using the pGV 1040 construct harboring spots per 0,5 cm2 of embryogenic tissue was obtained. GUS expression in proliferating somatic embryos has been observed after one and two months from bombardment.
650 $aEmbriogénese
653 $aCongresso
653 $aTecidos
700 1 $aGOMEZ, A.
700 1 $aCATANO, M. L.
700 1 $aHERRERA, P. V.
700 1 $aCALDERON, A.
700 1 $aMAYER, J. E.
700 1 $aROCA, W. M.
773 $tCali, Colombia: CIAT, 1993
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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