Data/hora: 09/06/2024 19:06:10

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 25/04/1994
Data da última atualização: | 25/04/1994
Autoria: | SORRIA, R. A.; GOMEZ, A.; CATANO, M. L.; HERRERA, P. V.; CALDERON, A.; MAYER, J. E.; ROCA, W. M.
Afiliação: | CIAT, Cali, Colombia.
Título: | Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation.
Ano de publicação: | 1993
Fonte/Imprenta: | Cali, Colombia: CIAT, 1993
Páginas: | 216-221p
Série: | CIAT,Working Document, 123
Idioma: | Inglês
Notas: | International scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedinjs... Cali, Colombia: CIAT, 1993.
Conteúdo: | Our strategy for Agrobacterium-mediated cassava transformation involves the utilization of cotyledonary leaves from somatic embryos and infection with both wild and disarmed strains. Virulence tests for wild Agrobacterium strains as well as regeneration protocols have been developed; the plasmid used in all the experiments was the pGV 1040 containing the uid A, the bar and the npt II genes (provided by Plant Genetic Systems, Gent). Sensitivity levels of cassava tissues to the commercial herbicide Basta, used as selection agent, were determined. Minimum lethal doses for the herbicide were 1 mg/l for immature leaves and 18mg/l for somatic embryos. Results with kanamyvin as selection agent were inconsistent. Only somatic embryos showed endogenous uid A gene exp  pression; endogenous activity was reduced by 70-80% using the kosugi protocol (1990), which includes methanol. Expression of opine synthesis genes of a wild Agobacterium strain, isolated from cassava tumors, was obtained after transformation of cotyledonary leaves of somatic embryos with the wild strain. Transformation experiments are now under way using the wild Agrobacterium strain carrying the pGV 1040 plasmid. A biolistic device was used to bombard early stage somatic embryos using the pGV 1040 construct harboring spots per 0,5 cm2 of embryogenic tissue was obtained. GUS expression in proliferating somatic embryos has been observed after one and two months from bombardment.
Palavras-Chave: | Congresso; Tecidos.
Thesagro: | Embriogénese.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13435-1) - ADD - PL - -- - 633.6820601 - I61p - 1993000070 - DIS