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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
13/08/2021 |
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Data da última atualização: |
13/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, T. R.; PAIXÃO, F. R. S. da; CATÃO, A. M. L.; MUNIZ, E. R.; RIBEIRO-SILVA, C. S.; TAVEIRA, S. F.; LUZ, C.; MASCARIN, G. M.; FERNANDES, E. K. K.; MARRETO, R. N. |
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Afiliação: |
THAINÁ RODRIGUES SANTOS, UFG; FLÁVIA REGINA SANTOS DA PAIXÃO, UFG; ALAINE MARIA LOPES CATÃO, UFG; ELEN REGOZINO MUNIZ, UFG; CÁRITA SOUZA RIBEIRO-SILVA, UFG; STEPHANIA FLEURY TAVEIRA, UFG; CHRISTIAN LUZ, UFG; GABRIEL MOURA MASCARIN, CNPMA; ÉVERTON KORT KAMP FERNANDES, UFG; RICARDO NEVES MARRETO, UFG. |
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Título: |
Inorganic pellets containing microsclerotia of Metarhizium anisopliae: a new technological platform for the biological control of the cattle tick Rhipicephalus microplus. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, V. 105, N. 12, p. 5001-5012, 2021. |
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ISSN: |
1432-0614 |
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DOI: |
https://doi.org/10.1007/s00253-021-11372-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study was sought to devise pellets containing inorganic materials and microsclerotia of Metarhizium anisopliae strain IP 119 for biological control of Rhipicephalus microplus, the most economically important tick in Brazilian cattle industry. In addition, we evaluated the storage stability of the pellets, their tolerance to ultraviolet radiation (UV-B), and efficacy against ticks under laboratory conditions. Fungal microsclerotia were produced by liquid culture fermentation and mixed with pre-selected inorganic matrices: vermiculite powder, diatomaceous earth, and colloidal silicon dioxide (78:20:2, w/w/w). The microsclerotial pellets were then prepared by a two-stage process involving extrusion and spheronization. Pellet size averaged 525.53 ± 7.74 micrometers, with a sphericity index of 0.72 ± 0.01, while biomass constituents did not affect the wet mass properties. Conidial production from microsclerotial pellets upon rehydration ranged from 1.85 × 10^9 to 1.97 × 10^9 conidia g-1 with conidial viability >= 93%. Conidial production from pellets stored at 4 °C was invariable for up to 21 days. Unformulated microsclerotia and microsclerotial pellets were extremely tolerant to UV-B compared with aerial conidia. Engorged tick females exposed to conidia from sporulated pellets applied to soil samples and upon optimal rehydration exhibited shorter oviposition time length, shorter life span, and reduced number of hatched larvae. In summary, microsclerotial pellets of M. anisopliae IP 119 effectively suppressed R. microplus and showed outstanding UV-B tolerance in laboratory tests. Prospectively, this formulation prototype is promising for targeting the non-parasitic stage of this tick on outdoor pasture fields and may offer a novel mycoacaricide for its sustainable management. MenosAbstract: This study was sought to devise pellets containing inorganic materials and microsclerotia of Metarhizium anisopliae strain IP 119 for biological control of Rhipicephalus microplus, the most economically important tick in Brazilian cattle industry. In addition, we evaluated the storage stability of the pellets, their tolerance to ultraviolet radiation (UV-B), and efficacy against ticks under laboratory conditions. Fungal microsclerotia were produced by liquid culture fermentation and mixed with pre-selected inorganic matrices: vermiculite powder, diatomaceous earth, and colloidal silicon dioxide (78:20:2, w/w/w). The microsclerotial pellets were then prepared by a two-stage process involving extrusion and spheronization. Pellet size averaged 525.53 ± 7.74 micrometers, with a sphericity index of 0.72 ± 0.01, while biomass constituents did not affect the wet mass properties. Conidial production from microsclerotial pellets upon rehydration ranged from 1.85 × 10^9 to 1.97 × 10^9 conidia g-1 with conidial viability >= 93%. Conidial production from pellets stored at 4 °C was invariable for up to 21 days. Unformulated microsclerotia and microsclerotial pellets were extremely tolerant to UV-B compared with aerial conidia. Engorged tick females exposed to conidia from sporulated pellets applied to soil samples and upon optimal rehydration exhibited shorter oviposition time length, shorter life span, and reduced number of hatched larvae. In summary, microsclerotial pellets o... Mostrar Tudo |
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Palavras-Chave: |
Bioefficacy. |
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Thesagro: |
Bovino; Carrapato; Controle Biológico; Fungo Entomógeno; Fungo Para Controle Biológico; Pellet. |
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Thesaurus Nal: |
Biological control; Entomopathogenic fungi; Pellets; Rhipicephalus microplus; Ticks. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03071naa a2200397 a 4500
001 2133550
005 2021-08-13
008 2021 bl uuuu u00u1 u #d
022 $a1432-0614
024 7 $ahttps://doi.org/10.1007/s00253-021-11372-1$2DOI
100 1 $aSANTOS, T. R.
245 $aInorganic pellets containing microsclerotia of Metarhizium anisopliae$ba new technological platform for the biological control of the cattle tick Rhipicephalus microplus.$h[electronic resource]
260 $c2021
520 $aAbstract: This study was sought to devise pellets containing inorganic materials and microsclerotia of Metarhizium anisopliae strain IP 119 for biological control of Rhipicephalus microplus, the most economically important tick in Brazilian cattle industry. In addition, we evaluated the storage stability of the pellets, their tolerance to ultraviolet radiation (UV-B), and efficacy against ticks under laboratory conditions. Fungal microsclerotia were produced by liquid culture fermentation and mixed with pre-selected inorganic matrices: vermiculite powder, diatomaceous earth, and colloidal silicon dioxide (78:20:2, w/w/w). The microsclerotial pellets were then prepared by a two-stage process involving extrusion and spheronization. Pellet size averaged 525.53 ± 7.74 micrometers, with a sphericity index of 0.72 ± 0.01, while biomass constituents did not affect the wet mass properties. Conidial production from microsclerotial pellets upon rehydration ranged from 1.85 × 10^9 to 1.97 × 10^9 conidia g-1 with conidial viability >= 93%. Conidial production from pellets stored at 4 °C was invariable for up to 21 days. Unformulated microsclerotia and microsclerotial pellets were extremely tolerant to UV-B compared with aerial conidia. Engorged tick females exposed to conidia from sporulated pellets applied to soil samples and upon optimal rehydration exhibited shorter oviposition time length, shorter life span, and reduced number of hatched larvae. In summary, microsclerotial pellets of M. anisopliae IP 119 effectively suppressed R. microplus and showed outstanding UV-B tolerance in laboratory tests. Prospectively, this formulation prototype is promising for targeting the non-parasitic stage of this tick on outdoor pasture fields and may offer a novel mycoacaricide for its sustainable management.
650 $aBiological control
650 $aEntomopathogenic fungi
650 $aPellets
650 $aRhipicephalus microplus
650 $aTicks
650 $aBovino
650 $aCarrapato
650 $aControle Biológico
650 $aFungo Entomógeno
650 $aFungo Para Controle Biológico
650 $aPellet
653 $aBioefficacy
700 1 $aPAIXÃO, F. R. S. da
700 1 $aCATÃO, A. M. L.
700 1 $aMUNIZ, E. R.
700 1 $aRIBEIRO-SILVA, C. S.
700 1 $aTAVEIRA, S. F.
700 1 $aLUZ, C.
700 1 $aMASCARIN, G. M.
700 1 $aFERNANDES, E. K. K.
700 1 $aMARRETO, R. N.
773 $tApplied Microbiology and Biotechnology, V. 105, N. 12, p. 5001-5012, 2021.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 27 | |
| 3. |  | LACORTE, C.; LOHUIS, D.; GOLDBACH, R.; PRINS, M. Spatial distribution and genetic stability of tobacco mosaic virus-based vectors in Nicotiana benthamiana plants. Virus: Reviews and Reserach, Búzios, v. 12, n. 1, p. 241, 2007.Suplemento. PV24. Virológica, 2007. Edição dos Resumos do XVIII National Meeting of Virology, Armação dos Búzios, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | RIBEIRO, S. G.; LOHUIS, H.; GOLDBACH, R.; PRINS, M. Tomato chlorotic mottle virus as target of RNA silencing in transgenic and non-transgenic host plants. Virus: Reviews and Reserach, Búzios, v. 12, n. 1, p. 72, 2007.Suplemento. Virológica, 2007. Edição dos Resumos do XVIII National Meeting of Virology, Armação dos Búzios, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | LACORTE, C.; RIBEIRO, S. G.; LOHUIS, H.; GOLDBACH, R.; PRINS, M. PVX-mediated expression of the N gene in Nicotiana benthamiana blocks Tomato spotted wilt virus. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 41., 2008, Belo Horizonte. [Resumos...]. Tropical Plant Pathology, v. 33, 2008. Suplemento. Resumo VIR-029. p. S132.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | RIBEIRO, S. G.; LACORTE, C.; BEENEN, H.; GOLDBACH, R.; PRINS, M. Transient expression of a single-chain antibody using PVX vector leads to enhanced resistance to Tomato spotted wilt virus. Fitopatologia Brasileira, v. 31, suppl., ago. 2006. Anais do: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 39.; ANNUAL MEETING OF THE BRAZILIAN PHYTOPATHOLOGICAL SOCIETY, 39., 2006, Salvador, BA. p. S291.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | AVILA, A. C. de; HAAN, P. de; KITAJIMA, E. W.; RESENDE, R. de O.; GOLDBACH, R. W.; PETERS, D. Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from impatiens in the Netherlands. Fitopatologia Brasileira, Brasilia, v.16, n.2, p.25, jun. 1991. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 27 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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