Data/hora: 05/06/2024 01:48:33

Biblioteca(s): 	Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Clima Temperado; Embrapa Hortaliças; Embrapa Trigo.	
Data corrente: 	12/03/1993	
Data da última atualização: 	10/03/2008	
Autoria: 	CASTRO, C.	
Título: 	Method for the quantitative estimation of Rhizoctonia solani in soil.	
Ano de publicação: 	1982	
Fonte/Imprenta: 	1982.	
Páginas: 	58 f.	
Idioma: 	Inglês	
Notas: 	Thesis (Doctor of Philosophy in Plant Pathology) - University of Idaho.	
Conteúdo: 	A reliable method for quantifying populations of Rhizoctonia solani Kuhn in soil is described. Field soil, treated with Prochloraz (Boots Company Ltd., Nottingham, England), is placed in the form of pellets on the medium of Ko and Hora amended with Prochloraz. After incubation, the perimeter of the soil pellets is examined for typical mycelium of Rhizoctonia spp. Each R. solany colony that develops is presumed to originate from a single  propagule or colony forming unit (CFU). The density of R. solani propagules in a soil sample is estimated by relating the CFU number to the amount of soil tested. To positively identify recovered isolates of R. solani, selected colonies from the Prochloraz-amended medium are stained and examined for nuclear condition. All multinucleate isolates are mated with known R. solani AG-types. Stewart's medium also is used to differentiate isolates of R. solani AG-3 fron other Rhizoctonia isolates. On this medium AG-3 colonies are normally brown while colonies of other Rhizoctonia AG-types are white. This procedure (Prochloraz modified soil and medium, i.e. "MSP method") was tested by estimating the populations of Rhizoctonia spp. in artificially and naturally infested soils. With both soil types, this procedure was more senitive and reliable than the existing pellet method without Prochloraz. The MSP method permits recovery of mycelia and sclerotia of R. solani independent of growth rate and AG-type. Because of the high sensitivity of this MSP method, it was possible to detect greater numbers of R. solani propagules in soil than was possible heretofore and to measure the effect of soil storage on propagule viability. It is believed that use of this MSP method should permit more accurate quantitative studies of R. solani populations in soil.	
Palavras-Chave: 	Plant diseases; Soilborne.	
Thesagro: 	Doença de Planta; Estimativa; Fungo; Método; Patógeno; População; Queima da Saia; Rhizoctonia Solani; Solo.	
Thesaurus Nal: 	fungi; pathogens; population; quantitative analysis; soil.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (16767-1) - ADD - TS - -- - T 056/82 - CAS - 1982.00056 - DIS
CPAC - (20183-1) - ADD - TS - -- - T017/82 - CAS - T017/82 - DIS
CPACT - (3977-1) - ADD - TS - -- - 00738 -  - 1982.00738 - DIS
CNPH - (619-1) - ADD - TS - PP - T CAS 35/82 -  - 1983.35 - DIS
CNPT - (30059-1) - ADD - TS - -- - TS-0479 -  - 0479 - DIS