| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
31/03/2008 |
|
Data da última atualização: |
26/09/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PACHECO, L. G. C.; PENA, R. R.; CASTRO, T. L. P.; DORELLA, F. A.; BAHIA, R. C.; CARMINATI, R.; FROTA, M. N. L.; OLIVEIRA, S. C.; MEYER, R.; ALVES, F. S. F.; MIYOSHI, A.; AZEVEDO, V. |
|
Afiliação: |
Luis G. C. Pacheco, Instituto de Ciências Biológicas UFMG; Roberta R. Pena, Instituto de Ciências Biológicas UFMG; Thiago L. P. Castro Instituto de Ciências Biológicas UFMG; Fernanda A. Dorella Instituto de Ciências Biológicas UFMG; Robson C. Bahia, UFBA-Departamento de Bio-Interação; Renato Carminati, UFBA-Departamento de Bio-Interação; Maurício N. L. Frota, UVA - Universidade Estadual Vale do Acaraú; Sérgio C. Oliveira, Instituto de Ciências Biológicas UFMG; Roberto Meyer, UFBA-Departamento de Bio-Interação; FRANCISCO SELMO FERNANDES ALVES, CNPC; Anderson Miyoshi, Instituto de Ciências Biológicas UFMG; Vasco Azevedo, Instituto de Ciências Biológicas UFMG. |
|
Título: |
Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Journal of Medical Microbiology, v. 56, n. 4, p. 480-486, Apr. 2007. |
|
DOI: |
10.1099/jmm.0.46997-0 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA. |
|
Palavras-Chave: |
CLA; PCR. |
|
Thesagro: |
Caprino; Corynebacterium Pseudotuberculosis; Linfadenite Caseosa; Ovino. |
|
Thesaurus Nal: |
caseous lymphadenitis. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/85860/1/API-Multiplex-PCR-assay.pdf
|
|
Marc: |
LEADER 02339naa a2200349 a 4500
001 1533624
005 2019-09-26
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1099/jmm.0.46997-0$2DOI
100 1 $aPACHECO, L. G. C.
245 $aMultiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples.$h[electronic resource]
260 $c2007
520 $aAbstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.
650 $acaseous lymphadenitis
650 $aCaprino
650 $aCorynebacterium Pseudotuberculosis
650 $aLinfadenite Caseosa
650 $aOvino
653 $aCLA
653 $aPCR
700 1 $aPENA, R. R.
700 1 $aCASTRO, T. L. P.
700 1 $aDORELLA, F. A.
700 1 $aBAHIA, R. C.
700 1 $aCARMINATI, R.
700 1 $aFROTA, M. N. L.
700 1 $aOLIVEIRA, S. C.
700 1 $aMEYER, R.
700 1 $aALVES, F. S. F.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
773 $tJournal of Medical Microbiology$gv. 56, n. 4, p. 480-486, Apr. 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Meio Ambiente. |
|
Data corrente: |
17/03/2021 |
|
Data da última atualização: |
17/03/2021 |
|
Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
|
Autoria: |
COSTA, P. da; COSTA, J. R. da; WANDELLI, E. V.; BIANCHINI, F.; TAVARES, E. D. |
|
Afiliação: |
PATRICIA DA COSTA, CNPMA; JOANNE REGIS DA COSTA, CPAA; ELISA VIEIRA WANDELLI, CPAA; FABRICIO BIANCHINI, CPATSA; EDSON DIOGO TAVARES, CPATC. |
|
Título: |
No poverty: contributions of Embrapa. |
|
Título original: |
Erradicação da pobreza: contribuições da Embrapa |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Brasília, DF: Embrapa, 2020. |
|
Descrição Física: |
E-book (pdf). |
|
Série: |
(Sustainable Development Goals, 1). |
|
ISBN: |
978-65-86056-03-7 |
|
Idioma: |
Inglês |
|
Notas: |
Translated by Paulo de Holanda Morais. |
|
Conteúdo: |
Poverty eradication, the focus of this book, is addressed in SDG 1 and considered the greatest challenge to achieve global targets. This SDG aims to end poverty in all its forms everywhere. This publication is on the contributions of Embrapa related to SDG targets 1.1, 1.2, and 1.5, understanding that poverty should not only be seen as a deprivation of financial resources, but also, to a large extent, the deprivation of resources, capacities, choices, security, and power. |
|
Palavras-Chave: |
Desigualdade social. |
|
Thesagro: |
Agricultura Familiar; Biomassa; Desenvolvimento Sustentável; Erradicação; Pobreza; Zoneamento Agrícola. |
|
Thesaurus NAL: |
Family farms; Poverty; Social inequality; Sustainable development. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01492nam a2200349 a 4500
001 2130763
005 2021-03-17
008 2020 bl uuuu 00u1 u #d
020 $a978-65-86056-03-7
100 1 $aCOSTA, P. da
240 $aErradicação da pobreza: contribuições da Embrapa
245 $aNo poverty$bcontributions of Embrapa.$h[electronic resource]
260 $aBrasília, DF: Embrapa$c2020
300 $cE-book (pdf).
490 $a(Sustainable Development Goals, 1).
500 $aTranslated by Paulo de Holanda Morais.
520 $aPoverty eradication, the focus of this book, is addressed in SDG 1 and considered the greatest challenge to achieve global targets. This SDG aims to end poverty in all its forms everywhere. This publication is on the contributions of Embrapa related to SDG targets 1.1, 1.2, and 1.5, understanding that poverty should not only be seen as a deprivation of financial resources, but also, to a large extent, the deprivation of resources, capacities, choices, security, and power.
650 $aFamily farms
650 $aPoverty
650 $aSocial inequality
650 $aSustainable development
650 $aAgricultura Familiar
650 $aBiomassa
650 $aDesenvolvimento Sustentável
650 $aErradicação
650 $aPobreza
650 $aZoneamento Agrícola
653 $aDesigualdade social
700 1 $aCOSTA, J. R. da
700 1 $aWANDELLI, E. V.
700 1 $aBIANCHINI, F.
700 1 $aTAVARES, E. D.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
