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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
15/09/2004 |
Data da última atualização: |
15/09/2004 |
Autoria: |
OVIEDO, V. R. S.; GODOY, A. R.; CASTRO, M. M.; CARDOSO, A. I. I. |
Título: |
Depresão endogâmica na produção de sementes de uma população de pepino japonês. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Horticultura Brasileira, Brasília, v. 22, n. 2, jul. 2004. Suplemento 2. |
Descrição Física: |
CD-ROM. |
Idioma: |
Português |
Notas: |
Trabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 358, jul. 2004. Suplemento 1. |
Palavras-Chave: |
Depressão endogâmica; Híbrido Natsu Suzumi. |
Thesagro: |
Autofecundação; Cucumis Sativus; Endogamia; Pepino; Qualidade; Semente. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00932naa a2200265 a 4500 001 1776689 005 2004-09-15 008 2004 bl uuuu u00u1 u #d 100 1 $aOVIEDO, V. R. S. 245 $aDepresão endogâmica na produção de sementes de uma população de pepino japonês. 260 $c2004 300 $cCD-ROM. 500 $aTrabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 358, jul. 2004. Suplemento 1. 650 $aAutofecundação 650 $aCucumis Sativus 650 $aEndogamia 650 $aPepino 650 $aQualidade 650 $aSemente 653 $aDepressão endogâmica 653 $aHíbrido Natsu Suzumi 700 1 $aGODOY, A. R. 700 1 $aCASTRO, M. M. 700 1 $aCARDOSO, A. I. I. 773 $tHorticultura Brasileira, Brasília$gv. 22, n. 2, jul. 2004. Suplemento 2.
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Embrapa Hortaliças (CNPH) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
02/01/2008 |
Data da última atualização: |
08/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
FERREIRA, C. R.; MEIRELLES, F. V.; YAMAZAKI, W.; CHIARATTI, M. R.; NICIURA, S. C. M.; PERECIN, F.; SMITH, L. C.; GARCIA, J. M. |
Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP; FLÁVIO VIEIRA MEIRELLES, USP; WALT YAMAZAKI, UNESP; MARCOS ROBERTO CHIARATTI, USP; SIMONE CRISTINA MEO NICIURA, CPPSE; FELIPE PERECIN, UNESP; LAWRENCE CHARLES SMITH, UNIVERSITÉ DE MONTRÉAL; JOAQUIM MANSANO GARCIA, UNESP. |
Título: |
The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Cloning and Stem Cells. v. 9, n. 4, p. 618-629, dec. 2007. |
DOI: |
10.1089/clo.2006.0082 |
Idioma: |
Inglês |
Conteúdo: |
The mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth. MenosThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either un... Mostrar Tudo |
Palavras-Chave: |
Bovine embryos; Cell mtDNA; Embryonic. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02452naa a2200253 a 4500 001 1048156 005 2023-03-08 008 2007 bl uuuu u00u1 u #d 024 7 $a10.1089/clo.2006.0082$2DOI 100 1 $aFERREIRA, C. R. 245 $aThe kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos.$h[electronic resource] 260 $c2007 520 $aThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth. 653 $aBovine embryos 653 $aCell mtDNA 653 $aEmbryonic 700 1 $aMEIRELLES, F. V. 700 1 $aYAMAZAKI, W. 700 1 $aCHIARATTI, M. R. 700 1 $aNICIURA, S. C. M. 700 1 $aPERECIN, F. 700 1 $aSMITH, L. C. 700 1 $aGARCIA, J. M. 773 $tCloning and Stem Cells.$gv. 9, n. 4, p. 618-629, dec. 2007.
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