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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
Data corrente: |
09/10/2013 |
Data da última atualização: |
09/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LIMA, A. A.; ARAGÃO, C. W. S.; CASTRO, M. E. B. de; OLIVEIRA, J. V. de C.; SOSA-GÓMEZ, D. R.; RIBEIRO, B. M. |
Afiliação: |
ANABELE AZEVEDO LIMA, UNB; CLARA WANDENKOLCK SILVA ARAGÃO, UNB; MARIA ELITA BATISTA DE CASTRO, CENARGEN; JULIANA VELASCO DE CASTRO OLIVEIRA, USP; DANIEL RICARDO SOSA GOMEZ, CNPSO; BERGMANN MORAIS RIBEIRO, UNB. |
Título: |
A recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
PLOSone, v. 8, n. 9, e74592, Sept. 2013. |
DOI: |
10.1371/journal.pone.0074592 |
Idioma: |
Inglês |
Conteúdo: |
One of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest. MenosOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed hi... Mostrar Tudo |
Thesagro: |
Soja. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/90688/1/journal.pone.0074592.pdf
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Marc: |
LEADER 02600naa a2200205 a 4500 001 1976569 005 2023-03-09 008 2013 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0074592$2DOI 100 1 $aLIMA, A. A. 245 $aA recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity.$h[electronic resource] 260 $c2013 520 $aOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest. 650 $aSoja 700 1 $aARAGÃO, C. W. S. 700 1 $aCASTRO, M. E. B. de 700 1 $aOLIVEIRA, J. V. de C. 700 1 $aSOSA-GÓMEZ, D. R. 700 1 $aRIBEIRO, B. M. 773 $tPLOSone$gv. 8, n. 9, e74592, Sept. 2013.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registros recuperados : 77 | |
1. | | CASTRO, M. E. B. de; SOUZA, M. L. de. Baculovírus: agentes de controle biológico. In: OLIVEIRA-FILHO, E. C.; MONNERAT, R. G. (Ed.). Fundamentos para a regulação de semioquímicos, inimigos naturais e agentes microbiológicos de controle de pragas. Planaltina, DF: Embrapa Cerrados, 2006. p. 175-194.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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6. | | CRAVEIRO, S. R.; RIBEIRO, Z. M. de A.; INGLIS, P. W.; CASTRO, M. E. B. de. Análise filogenética de Pseudoplusia includens SNPV-IE baseada nos genes conservados lef-8 e polh. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 057.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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9. | | ALMEIDA, G. F.; PAULA, D. P.; SOUZA, M. L. de; CASTRO, M. E. B. de. Clonagem e sequenciamento de um gene de virulência (P74) de um vírus isolado da lagarta-do-álamo Condylorrhiza vestigialis. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. ResumoID:1991-1.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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11. | | CASTRO, M. E. B. de; RIBEIRO, Z. M. de A.; SIHLER, W.; SOUZA, M. L. de. Coleção de vírus de invertebrados para uso em controle biológico. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. CD-ROMTipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | FERREIRA, B. C.; CASTRO, M. E. B. de; PINEDO, F. J. R.; SOUZA, M. L. de. Construção de um vírus recombinante de Anticarsia gemmatalis MNPV com interrupção do gene pif-1. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 061. p. 102Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | FERREIRA, B. C.; CASTRO, M. E. B. de; PINEDO, F. J. R.; SOUZA, M. L. de. Construction of a recombinant Anticarsia Gemmatalis MNPV with interruption of the PIF-1 gene (PER OS INFECTIVITY FACTOR). Virus Reviews & Research, São Paulo, v. 14, Supl. 1, p. 201, nov. 2009. Edição dos resumos do 20. National Meeting of Virology. Brasília, DF, nov. 2009.Tipo: Resumo em Anais de Congresso |
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