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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Amazônia Ocidental. |
Data corrente: |
06/03/2023 |
Data da última atualização: |
01/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CASTRO, G. S.; SOUZA, T. F.; SILVA, G. F. da; PEDROSO, R. C. N.; MENEZES, K. S.; SOARES, M. A.; DIAS, G. M.; SANTOS, A. O.; YAMAGISHI, M. E. B.; FARIA, J. V.; JANUÁRIO, A. H.; KOOLEN, H. H. F. |
Afiliação: |
GLEUCINEI S. CASTRO, UNIVERSIDADE DO ESTADO DO AMAZONAS; THIAGO F. SOUSA, UNIVERSIDADE FEDERAL DO AMAZONAS; GILVAN FERREIRA DA SILVA, CPAA; RITA C. N. PEDROSO, UNIVERSIDADE DE FRANCA; KELLY S. MENEZES, UNIVERSIDADE DO ESTADO DO AMAZONAS; MARCOS A. SOARES, UNIVERSIDADE FEDERAL DE MATO GROSSO; GUSTAVO M. DIAS, UNIVERSIDADE FEDERAL DO ABC; ALINE O. SANTOS, UNIVERSIDADE DO ESTADO DO AMAZONAS; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; JÉSSICA V. FARIA, UNIVERSIDADE DO ESTADO DO AMAZONAS; ANA H. JANUÁRIO, UNIVERSIDADE DE FRANCA; HECTOR H. F. KOOLEN, UNIVERSIDADE DO ESTADO DO AMAZONAS. |
Título: |
Characterization of peptaibols produced by a marine strain of the fungus Trichoderma endophyticum via Mass Spectrometry, genome mining and phylogeny-based prediction. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Metabolites, v. 13, n. 2, 221, Feb. 2023. |
DOI: |
https://doi.org/10.3390/metabo13020221 |
Idioma: |
Inglês |
Conteúdo: |
Abstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for peptaibol diversification. This study demonstrates the importance of combining genome mining techniques, mass spectrometry analysis and molecular networks for the discovery of new natural products. MenosAbstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for pept... Mostrar Tudo |
Palavras-Chave: |
De novo sequencing; Fungo marinho; Marine fungus; Molecular networking; Nonribosomal peptides; Peptídeos não ribossomais; Rede molecular; Synteny analysis; Tricoderma. |
Thesagro: |
Peptídeo. |
Thesaurus Nal: |
Hypocreaceae. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152102/1/metabolites-13-00221-v2.pdf
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Marc: |
LEADER 02911naa a2200397 a 4500 001 2152102 005 2023-09-01 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/metabo13020221$2DOI 100 1 $aCASTRO, G. S. 245 $aCharacterization of peptaibols produced by a marine strain of the fungus Trichoderma endophyticum via Mass Spectrometry, genome mining and phylogeny-based prediction.$h[electronic resource] 260 $c2023 520 $aAbstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for peptaibol diversification. This study demonstrates the importance of combining genome mining techniques, mass spectrometry analysis and molecular networks for the discovery of new natural products. 650 $aHypocreaceae 650 $aPeptídeo 653 $aDe novo sequencing 653 $aFungo marinho 653 $aMarine fungus 653 $aMolecular networking 653 $aNonribosomal peptides 653 $aPeptídeos não ribossomais 653 $aRede molecular 653 $aSynteny analysis 653 $aTricoderma 700 1 $aSOUZA, T. F. 700 1 $aSILVA, G. F. da 700 1 $aPEDROSO, R. C. N. 700 1 $aMENEZES, K. S. 700 1 $aSOARES, M. A. 700 1 $aDIAS, G. M. 700 1 $aSANTOS, A. O. 700 1 $aYAMAGISHI, M. E. B. 700 1 $aFARIA, J. V. 700 1 $aJANUÁRIO, A. H. 700 1 $aKOOLEN, H. H. F. 773 $tMetabolites$gv. 13, n. 2, 221, Feb. 2023.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja; Embrapa Trigo. |
Data corrente: |
04/11/2009 |
Data da última atualização: |
12/10/2010 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
GODOY, C. V.; SILVA, L. H. C. P. da; UTIAMADA, C. M.; SIQUERI, F. V.; LOPES, I. de O. N.; ROESE, A. D.; MACHADO, A. Q.; FORCELINI, C. A.; PIMENTA, C. B.; NUNES, C. D. M.; CASSETARI NETO, D.; JACCOUD FILHO, D. S.; FORNAROLLI, D. A.; MIGUEL-WRUCK D. S.; RAMOS JÚNIOR, E. U.; BORGES, E. P.; JULIATTI, F. C.; FEKSA, H. R.; CAMPOS, H. D.; NUNES JÚNIOR, J.; SILVA, J. R. C.; COSTAMILAN, L. M.; CARNEIRO, L. C.; SATO, L. N.; CANTERI, M. G.; ITO, M. A.; IAMAMOTO, M. M.; ITO, M. F.; MEYER, M. C.; COSTA, M. J. N. da; DIAS, M. D.; MARTINS, M. C.; LOPES, P. V. L.; SOUZA, P. I. M.; BARROS, R.; BALARDIN, R. S.; IGARASHI, S.; SILVA, S. A. da; FURLAN, S. H.; CARLIN, V. J. |
Afiliação: |
CLAUDIA VIEIRA GODOY, CNPSo; LUIS HENRIQUE C. P. DA SILVA, FESURV; CARLOS M. UTIAMADA, TAGRO; FABIANO V. SIQUERI, Fundação Mato Grosso; IVANI DE OLIVEIRA NEGRAO LOPES, CNPSo; ALEXANDRE DINNYS ROESE, CPAO; ANDRÉIA Q. MACHADO, UNIVAG; CARLOS A. FORCELINI, UPF; CLÁUDIA B. PIMENTA, SEAGRO; CLEY DONIZETI MARTINS NUNES, CPACT; DANIEL CASSETARI NETO, UFMT; DAVID S. JACCOUD FILHO, UEPG; DONIZETI A. FORNAROLLI, Faculdade Integrado de Campo Mourão; DULÂNDULA S. MIGUEL-WRUCK, EPAMIG; EDISON U. RAMOS JÚNIOR, Apta Regional do Sudoeste Paulista; EDSON P. BORGES, Fundação Chapadão; FERNANDO C. JULIATTI, Universidade Federal de Uberlândia; HERALDO R. FEKSA, Fundação Agrária de Pesquisa Agrocuária; HERCULES D. CAMPOS, FESURV; JOSÉ NUNES JÚNIOR, Centro Tecnológico para Pesquisas Agropecuárias; JULIANA R. C. SILVA, CARREGAL; LEILA MARIA COSTAMILAN, CNPT; LUCIANA C. CARNEIRO, UFGO; LUIZ NOBUO SATO, TAGRO; MARCELO G. CANTERI, UEL; MARCIO A. ITO, Apta Regional do Sudoeste Paulista; MARCOS M. IAMAMOTO, MClamamoto Assessoria em Fitopatologia; MARGARIDA F. ITO, IAC; MAURICIO CONRADO MEYER, CNPSo; MAURO JUNIOR N. DA COSTA, IAC; MOAB D. DIAS, UFT; MÔNICA C. MARTINS, Fundação de Apoio à Pesquisa e Desenvolvimento do Oeste Baiano; PEDRO V. L. LOPES, Fundação de apoio à Pesquisa e Desenvolvimento do Oeste Baiano; PLINIO ITAMAR DE MELLO DE SOUZA, CPAC; RICARDO BARROS, Fundação MS para Pesquisa e Difusão de Tecnologias Agropecuárias; RICARDO S. BALARDIN, UFSM; SEIJI IGARASHI, UEL; SERGIO ABUD DA SILVA, CPAC; SILVÂNIA H. FURLAN, Instituto Biológico; VALTEMIR J. CARLIN, Agrodinâmica. |
Título: |
Eficiência de fungicidas para controle da ferrugem asiática da soja, Phakopsora pachyrhizi, na safra 2008/09. Resultados sumarizados dos ensaios cooperativos. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: REUNIÃO DO CONSÓRCIO ANTIFERRUGEM SAFRA 2008-09, 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 71-87. (Embrapa Soja. Documentos, 315). Editado por Claudia Vieira Godoy, Claudine Dinali Santos Seixas, Rafael Moreira Soares. |
Idioma: |
Português |
Palavras-Chave: |
Fitopatologia; Plant disease. |
Thesagro: |
Doença de planta; Ferrugem; Fungicida; Glycine Max; Phakopsora Pachyrhizi; Soja. |
Thesaurus NAL: |
Fungicides; Rust diseases; Soybeans. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02157naa a2200709 a 4500 001 1852764 005 2010-10-12 008 2009 bl uuuu u00u1 u #d 100 1 $aGODOY, C. V. 245 $aEficiência de fungicidas para controle da ferrugem asiática da soja, Phakopsora pachyrhizi, na safra 2008/09. Resultados sumarizados dos ensaios cooperativos. 260 $c2009 650 $aFungicides 650 $aRust diseases 650 $aSoybeans 650 $aDoença de planta 650 $aFerrugem 650 $aFungicida 650 $aGlycine Max 650 $aPhakopsora Pachyrhizi 650 $aSoja 653 $aFitopatologia 653 $aPlant disease 700 1 $aSILVA, L. H. C. P. da 700 1 $aUTIAMADA, C. M. 700 1 $aSIQUERI, F. V. 700 1 $aLOPES, I. de O. N. 700 1 $aROESE, A. D. 700 1 $aMACHADO, A. Q. 700 1 $aFORCELINI, C. A. 700 1 $aPIMENTA, C. B. 700 1 $aNUNES, C. D. M. 700 1 $aCASSETARI NETO, D. 700 1 $aJACCOUD FILHO, D. S. 700 1 $aFORNAROLLI, D. A. 700 1 $aMIGUEL-WRUCK D. S. 700 1 $aRAMOS JÚNIOR, E. U. 700 1 $aBORGES, E. P. 700 1 $aJULIATTI, F. C. 700 1 $aFEKSA, H. R. 700 1 $aCAMPOS, H. D. 700 1 $aNUNES JÚNIOR, J. 700 1 $aSILVA, J. R. C. 700 1 $aCOSTAMILAN, L. M. 700 1 $aCARNEIRO, L. C. 700 1 $aSATO, L. N. 700 1 $aCANTERI, M. G. 700 1 $aITO, M. A. 700 1 $aIAMAMOTO, M. M. 700 1 $aITO, M. F. 700 1 $aMEYER, M. C. 700 1 $aCOSTA, M. J. N. da 700 1 $aDIAS, M. D. 700 1 $aMARTINS, M. C. 700 1 $aLOPES, P. V. L. 700 1 $aSOUZA, P. I. M. 700 1 $aBARROS, R. 700 1 $aBALARDIN, R. S. 700 1 $aIGARASHI, S. 700 1 $aSILVA, S. A. da 700 1 $aFURLAN, S. H. 700 1 $aCARLIN, V. J. 773 $tIn: REUNIÃO DO CONSÓRCIO ANTIFERRUGEM SAFRA 2008-09, 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 71-87. (Embrapa Soja. Documentos, 315). Editado por Claudia Vieira Godoy, Claudine Dinali Santos Seixas, Rafael Moreira Soares.
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