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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Clima Temperado; Embrapa Hortaliças; Embrapa Trigo. |
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Data corrente: |
12/03/1993 |
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Data da última atualização: |
10/03/2008 |
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Autoria: |
CASTRO, C. |
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Título: |
Method for the quantitative estimation of Rhizoctonia solani in soil. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
1982. |
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Páginas: |
58 f. |
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Idioma: |
Inglês |
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Notas: |
Thesis (Doctor of Philosophy in Plant Pathology) - University of Idaho. |
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Conteúdo: |
A reliable method for quantifying populations of Rhizoctonia solani Kuhn in soil is described. Field soil, treated with Prochloraz (Boots Company Ltd., Nottingham, England), is placed in the form of pellets on the medium of Ko and Hora amended with Prochloraz. After incubation, the perimeter of the soil pellets is examined for typical mycelium of Rhizoctonia spp. Each R. solany colony that develops is presumed to originate from a single propagule or colony forming unit (CFU). The density of R. solani propagules in a soil sample is estimated by relating the CFU number to the amount of soil tested. To positively identify recovered isolates of R. solani, selected colonies from the Prochloraz-amended medium are stained and examined for nuclear condition. All multinucleate isolates are mated with known R. solani AG-types. Stewart's medium also is used to differentiate isolates of R. solani AG-3 fron other Rhizoctonia isolates. On this medium AG-3 colonies are normally brown while colonies of other Rhizoctonia AG-types are white. This procedure (Prochloraz modified soil and medium, i.e. "MSP method") was tested by estimating the populations of Rhizoctonia spp. in artificially and naturally infested soils. With both soil types, this procedure was more senitive and reliable than the existing pellet method without Prochloraz. The MSP method permits recovery of mycelia and sclerotia of R. solani independent of growth rate and AG-type. Because of the high sensitivity of this MSP method, it was possible to detect greater numbers of R. solani propagules in soil than was possible heretofore and to measure the effect of soil storage on propagule viability. It is believed that use of this MSP method should permit more accurate quantitative studies of R. solani populations in soil. MenosA reliable method for quantifying populations of Rhizoctonia solani Kuhn in soil is described. Field soil, treated with Prochloraz (Boots Company Ltd., Nottingham, England), is placed in the form of pellets on the medium of Ko and Hora amended with Prochloraz. After incubation, the perimeter of the soil pellets is examined for typical mycelium of Rhizoctonia spp. Each R. solany colony that develops is presumed to originate from a single propagule or colony forming unit (CFU). The density of R. solani propagules in a soil sample is estimated by relating the CFU number to the amount of soil tested. To positively identify recovered isolates of R. solani, selected colonies from the Prochloraz-amended medium are stained and examined for nuclear condition. All multinucleate isolates are mated with known R. solani AG-types. Stewart's medium also is used to differentiate isolates of R. solani AG-3 fron other Rhizoctonia isolates. On this medium AG-3 colonies are normally brown while colonies of other Rhizoctonia AG-types are white. This procedure (Prochloraz modified soil and medium, i.e. "MSP method") was tested by estimating the populations of Rhizoctonia spp. in artificially and naturally infested soils. With both soil types, this procedure was more senitive and reliable than the existing pellet method without Prochloraz. The MSP method permits recovery of mycelia and sclerotia of R. solani independent of growth rate and AG-type. Because of the high sensitivity of this MSP metho... Mostrar Tudo |
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Palavras-Chave: |
Plant diseases; Soilborne. |
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Thesagro: |
Doença de Planta; Estimativa; Fungo; Método; Patógeno; População; Queima da Saia; Rhizoctonia Solani; Solo. |
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Thesaurus Nal: |
fungi; pathogens; population; quantitative analysis; soil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02625nam a2200325 a 4500
001 1207862
005 2008-03-10
008 1982 bl uuuu m 00u1 u #d
100 1 $aCASTRO, C.
245 $aMethod for the quantitative estimation of Rhizoctonia solani in soil.
260 $a1982.$c1982
300 $a58 f.
500 $aThesis (Doctor of Philosophy in Plant Pathology) - University of Idaho.
520 $aA reliable method for quantifying populations of Rhizoctonia solani Kuhn in soil is described. Field soil, treated with Prochloraz (Boots Company Ltd., Nottingham, England), is placed in the form of pellets on the medium of Ko and Hora amended with Prochloraz. After incubation, the perimeter of the soil pellets is examined for typical mycelium of Rhizoctonia spp. Each R. solany colony that develops is presumed to originate from a single propagule or colony forming unit (CFU). The density of R. solani propagules in a soil sample is estimated by relating the CFU number to the amount of soil tested. To positively identify recovered isolates of R. solani, selected colonies from the Prochloraz-amended medium are stained and examined for nuclear condition. All multinucleate isolates are mated with known R. solani AG-types. Stewart's medium also is used to differentiate isolates of R. solani AG-3 fron other Rhizoctonia isolates. On this medium AG-3 colonies are normally brown while colonies of other Rhizoctonia AG-types are white. This procedure (Prochloraz modified soil and medium, i.e. "MSP method") was tested by estimating the populations of Rhizoctonia spp. in artificially and naturally infested soils. With both soil types, this procedure was more senitive and reliable than the existing pellet method without Prochloraz. The MSP method permits recovery of mycelia and sclerotia of R. solani independent of growth rate and AG-type. Because of the high sensitivity of this MSP method, it was possible to detect greater numbers of R. solani propagules in soil than was possible heretofore and to measure the effect of soil storage on propagule viability. It is believed that use of this MSP method should permit more accurate quantitative studies of R. solani populations in soil.
650 $afungi
650 $apathogens
650 $apopulation
650 $aquantitative analysis
650 $asoil
650 $aDoença de Planta
650 $aEstimativa
650 $aFungo
650 $aMétodo
650 $aPatógeno
650 $aPopulação
650 $aQueima da Saia
650 $aRhizoctonia Solani
650 $aSolo
653 $aPlant diseases
653 $aSoilborne
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 5.798 | |
| 6. |  | CASTRO, N. R. A. Análise de risco de pragas. In: CURSO SOBRE TECNOLOGIA DE PRODUÇÃO DE SEMENTES DE HORTALIÇAS, 8., 2008, Brasília, DF. Palestras... Brasília, DF: Embrapa Hortaliças, 2008. CD-ROM.| Biblioteca(s): Embrapa Hortaliças. |
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| 17. | | CASTRO, C. M. Batata o pão nosso das Américas. In: BARBIERI, R. L.; STUMPF, E. R. T. (Ed.). Origem e evolução de plantas cultivadas. Brasília, DF: Embrapa Informação Tecnológica; Pelotas: Embrapa Clima Temperado, 2008. p. 219-234| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 5.798 | |
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